Purification of anthocyanins from species of<i> Banksia</i> and<i> Acacia</i> using high‐voltage paper electrophoresis

Asenstorfer, Robert E.; Morgan, Anne L.; Hayasaka, Yoji; Sedgley, Margaret; Jones, Graham P.

doi:10.1002/pca.696

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…are a relatively rich source of anthocyanin flavonoids. 22 Flavonoids have well established bacterial growth inhibitory activities. 23 The flavonoids kaempferol and myricetin have been reported to be potent growth inhibitors of a panel of bacterial pathogens.…”

Section: Discussionmentioning

confidence: 99%

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The Inhibitory Activity of Banksia collina R.Br. and Banksia oblongifolia Cav. Methanolic Leaf Extracts against a Panel of Bacterial Pathogens

Cock¹

2019

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Introduction: The development of multi-antibiotic resistant strains of bacteria has necessitated the search for new, effective antibacterial therapies. B. collina and B. oblongifolia leaves were used by Australian Aborigines to treat bacterial infections. However, little research has been published on antibacterial activity of these species. Methods: The ability of B. collina and B. oblongifolia leaf extracts to inhibit the growth of gram-negative and gram-positive bacterial species was investigated by disc diffusion and growth time course assays. The growth inhibitory activity was further quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: The B. collina and B. oblongifolia leaf extracts were good inhibitors of the growth of both gram-positive and gram-negative bacteria. The B. collina and B. oblongifolia leaf extracts were particularly good inhibitors of A. faecalis growth (MICs of 225 and 486μg/ mL respectively) and B. cereus growth (MICs of 515 and 875μg/mL respectively). The B. collina extract was also a good inhibitor of B. subtilis growth, whilst the B. oblongifolia extract was a moderate growth inhibitor (MIC values of 923 and 1250µg/mL respectively). A similar, trend was noted for Y. entercolitica growth inhition (MICs of 518 and 1136μg/mL respectively). Whilst MIC values were also determined against other bacterial species, they generally indicated low-moderate activity. The B. collina and B. oblongifolia leaf extracts were further investigated by growth time course assays against A. faecalis and B. cereus. Interestingly, both extracts showed significant growth inhibition within 1h of exposure against both bacterial species. All extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for the treatment of bacterial infections. Conclusion: The lack of toxicity of the B. collina and B. oblongifolia leaf extracts and their growth inhibitory bioactivity against multiple bacterial species indicate their potential in the development of new antibiotic chemotherapies.

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Section: Discussionmentioning

confidence: 99%

“…and Banksia menziesii R.Br. 22 These species contain an abundance of anthocyanins including cyanidin-3-galactoside ( Figure 1c), cyanidin-3-glucoside ( Figure 1d), cyaniding-3,5-diglucoside Figure 1e), peonidin-3-galactoside ( Figure 1f) and peonidin-3-glucoside ( Figure 1g). Many similar flavonoids have good antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Activity of Banksia collina R.Br. and Banksia oblongifolia Cav. Methanolic Leaf Extracts against a Panel of Bacterial Pathogens

Cock¹

2019

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“…The long-standing horticultural use of Protea provides evidence for a heritable component to floral colour differences [38,44,46], although the genetics and pigments are as yet undescribed. In two other Proteaceae species, pink-flowered Banksia menziesii and scarlet-flowered Banksia coccinea have the same two glycosides of cyanidin but had a different glycoside of paeonidin [47].…”

Section: Materials and Methods (A) Study Speciesmentioning

confidence: 99%

Extrapolating from local ecological processes to genus-wide patterns in colour polymorphism in South AfricanProtea

Carlson

Holsinger

2015

Proc. R. Soc. B.

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Polymorphic traits are central to many fundamental discoveries in evolution, yet why they are found in some species and not others remains poorly understood. We use the African genus Protea-within which more than 40% of species have co-occurring pink and white floral colour morphs-to ask whether convergent evolution and ecological similarity could explain the genus-wide pattern of polymorphism. First, we identified environmental correlates of pink morph frequency across 28 populations of four species. Second, we determined whether the same correlates could predict species-level polymorphism and monomorphism across 31 species. We found that pink morph frequency increased with elevation in Protea repens and three section Exsertae species, increased eastward in P. repens, and increased with seed predation intensity in section Exsertae. For cross-species comparisons, populations of monomorphic pink species occurred at higher elevations than populations of monomorphic white species, and 18 polymorphic species spanned broader elevational gradients than 13 monomorphic species. These results suggest that divergent selection along elevational clines has repeatedly favoured polymorphism, and that more uniform selection in altitudinally restricted species may promote colour monomorphism. Our findings are, to our knowledge, the first to link selection acting within species to the presence and absence of colour polymorphism at broader phylogenetic scales.

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“…e methods for purifying anthocyanins include adsorption on macroporous resins [17][18][19], liquid and subcritical carbon dioxide [20], membrane separation [21], preparative high-performance liquid chromatography [22], aqueous two-phase extraction [23,24], ionic liquid solutions [25], high-speed countercurrent chromatography [26], high voltage paper electrophoresis [27], and column chromatography [28]. ese methods can achieve high purity, but many are laboratory-level and cannot be industrialized.…”

Section: Introductionmentioning

confidence: 99%

Purification of Anthocyanins Derived from Black Kidney Bean (Phaseolus vulgaris L.) by a Simulated Moving Bed

Cheng

Zheng

2021

Journal of Chemistry

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Purification of anthocyanins derived from black kidney bean (Phaseolus vulgaris L.) by column chromatography and simulated moving bed (SMB) methods was investigated, and the anthocyanins of black kidney bean were identified. The SMB had advantages over column chromatography in processing efficiency, operation cost, and automation degree in contrast testing. The best SMB conditions resulted in purity and yield of black kidney bean anthocyanins of 24.61 ± 0.21% and 87.85 ± 0.32%, respectively. The half maximal inhibitory concentration (IC50) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and 2,2ʹ-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS+∙) scavenging activity was 0.95 and 2.14 of refined anthocyanins, respectively, indicating strong antioxidant capacity. Three anthocyanins were detected and identified by UPLC-Triple-TOF/MS from black kidney bean skins: delphinidin-3-O-glucoside, petunidin-3-O-glucoside, and malvidin-3-O-glucoside. The experimental results suggested that SMB may help promote industrialization and purification of anthocyanins from colored kidney beans as well as from other plant materials.

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Purification of anthocyanins from species of Banksia and Acacia using high‐voltage paper electrophoresis

Cited by 9 publications

References 10 publications

The Inhibitory Activity of Banksia collina R.Br. and Banksia oblongifolia Cav. Methanolic Leaf Extracts against a Panel of Bacterial Pathogens

The Inhibitory Activity of Banksia collina R.Br. and Banksia oblongifolia Cav. Methanolic Leaf Extracts against a Panel of Bacterial Pathogens

Extrapolating from local ecological processes to genus-wide patterns in colour polymorphism in South AfricanProtea

Purification of Anthocyanins Derived from Black Kidney Bean (Phaseolus vulgaris L.) by a Simulated Moving Bed

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