Purification of an osmotin-like protein from the seeds of Benincasa hispida and cloning of the gene encoding this protein

Shih, Chao-Yun T.; Wu, Junlin; Jia, Shifang; Khan, Anwar; Ting, Kwun-Lon; Shih, Ding S.

doi:10.1016/s0168-9452(00)00450-7

Cited by 33 publications

(31 citation statements)

References 25 publications

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“…The oil from the seeds is soporific, good for the brain and liver, and also useful in the treatment of syphilis (Qadrie, Hawisa, Khan, Samuel, & Anandan, 2009). Besides, Lee, Choi, and Kim (2005) reported that the seed also contains small amounts of a triterpenoid known as isomultiflorenol, proteins such as trigonelline, coffearin, and osmotin (Shih et al, 2001), steroids such as β-sitosterol and stigmast-5-ene-3-beta-ol, alkaloids such as 5-methylcytosine, and triterpenoids such as cucurbitacin B (Uchikoba, Yonezawa, & Kaneda, 1998).…”

Section: Miscellaneous Compoundsmentioning

confidence: 99%

Kundur [Benincasa hispida (Thunb.) Cogn.]: A potential source for valuable nutrients and functional foods

Zaini

Anwar

Hamid

et al. 2011

Food Research International

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Section: Miscellaneous Compoundsmentioning

confidence: 99%

Kundur [Benincasa hispida (Thunb.) Cogn.]: A potential source for valuable nutrients and functional foods

Zaini

Anwar

Hamid

et al. 2011

Food Research International

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“…This sweettasting protein belongs to the PR-5 family of antifungal proteins (Malehorn et al 1994;Sassa and Hirano 1998). Several members of the PR-5-protein group of tobacco and other plants significantly inhibited spore germination or hyphal growth of various fungi, probably by permeabilizing fungal membranes (Stintzi et al 1993;Sassa and Hirano 1998;Shih et al 2001). PR-5-proteins also act as antifreeze-glycoproteins inhibiting growth under extreme cold.…”

Section: Fine Map Around Foc-4 and Foc-5 Locimentioning

confidence: 99%

“…PR-5-proteins also act as antifreeze-glycoproteins inhibiting growth under extreme cold. Southern hybridization data indicated that these proteins are encoded by single-copy genes (Shih et al 2001). The two adjacent markers OP-P15-3 and OP-P15-4 (co-segregating in the analyzed population and appearing as two alleles of the same locus) were of very different sequence composition.…”

Section: Fine Map Around Foc-4 and Foc-5 Locimentioning

confidence: 99%

Molecular markers closely linked to fusarium resistance genes in chickpea show significant alignments to pathogenesis-related genes located on Arabidopsis chromosomes 1 and 5

et al. 2003

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A population of 131 recombinant inbred lines from a wide cross between chickpea ( Cicer arietinum L., resistant parent) and Cicer reticulatum (susceptible parent) segregating for the closely linked resistances against Fusarium oxysporum f.sp. ciceri races 4 and 5 was used to develop DNA amplification fingerprinting markers linked to both resistance loci. Bulked segregant analysis revealed 19 new markers on linkage group 2 of the genetic map on which the resistance genes are located. Closest linkage (2.0 cM) was observed between marker R-2609-1 and the race 4 resistance locus. Seven other markers flanked this locus in a range from 4.1 to 9.0 cM. These are the most closely linked markers available for this locus up to date. The sequences of the linked markers were highly similar to genes encoding proteins involved in plant pathogen response, such as a PR-5 thaumatin-like protein and an important regulator of the phytoalexin pathway, anthranilate N-hydroxycinnamoyl-benzoyltransferase. Others showed significant alignments to genes encoding housekeeping enzymes such as the MutS2 DNA-mismatch repair protein. In the Arabidopsis genome, similar genes are located on short segments of chromosome 1 and 5, respectively, suggesting synteny between the fusarium resistance gene cluster of chickpea and the corresponding regions in the Arabidopsis genome. Three marker sequences were similar to retrotransposon-derived and/or satellite DNA sequences. The markers developed here provide a starting point for physical mapping and map-based cloning of the fusarium resistance genes and exploration of synteny in this highly interesting region of the chickpea genome.

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“…In the tobacco PR-5 gene, cis-elements involved in tobacco mosaic virus-inducible expression were found (Albrecht et al, 1992). In a Benincasa hispida TL gene, c/s-elements important for transcription such as TATA box and CAAT box were predicted (Shih et al, 2001). Sassa et al (2002) have reported a pistil-specific TL promoter in the Japanese pear with motif Ill-related sequences that are found in the promoters of selfincompatibility genes.…”

mentioning

confidence: 99%

Molecular characterization of a fruit-preferential thaumatin-like gene from apple (Malus domestica cv. Fuji)

2003

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Functioning of the thaumatin-like (TL) protein is known to be pathogenesis-or stress-related. Here we identified TL protein cDNA, from an apple-skin library, that was nearly identical to that of the MdTL1 gene. Transcripts of this so-named gene, MdTLla, were highly expressed in the fruit, but rarely in other tissue types. Expression was found in both the skin and the flesh of the fruit. We also examined the environmental or hormonal control of MdTLla expression. Exposing the fruit to light caused this gene to be induced in the skin tissues. Accumulation of IHdTLla mRNA reached a peak between Days I and 5 after exposure. In the leaves, MdTLIa was induced by salicylic acid (SA), but was not significantly affected by any other stresses. Analysis of the IHdTLla genomic clone, 2~TL1, revealed that transcription of MdTLla begins 53 bp upstream of the start codon. Sequence analysis of the ca. 1.0-kb MdTLla promoter region has enabled us to predict that it has a stress-related cis-element, such as the ABA responsive element (ABRE), as well as a light-responsive GT-1 and I-box.

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Purification of an osmotin-like protein from the seeds of Benincasa hispida and cloning of the gene encoding this protein

Cited by 33 publications

References 25 publications

Kundur [Benincasa hispida (Thunb.) Cogn.]: A potential source for valuable nutrients and functional foods

Kundur [Benincasa hispida (Thunb.) Cogn.]: A potential source for valuable nutrients and functional foods

Molecular markers closely linked to fusarium resistance genes in chickpea show significant alignments to pathogenesis-related genes located on Arabidopsis chromosomes 1 and 5

Molecular characterization of a fruit-preferential thaumatin-like gene from apple (Malus domestica cv. Fuji)

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