Purification of aflatoxin B1 antibody for the development of aflatoxin biosensor

Prihantoro, E. A. B.; Saepudin, Endang; Ivandini, Tribidasari A.

doi:10.1063/1.4991180

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Aflatoxin from A. flavus was supplied by Sigma Aldrich, USA. The aflatoxin antibody (anti-AFM1) was made in our laboratory (Assaat et al, 2018) and purified as described by Prihantoro et al (2017). Nitrocellulose membrane, Tween® 20, sucrose, sodium azide, Cd(NO3)2, Na2S, sodium dioctyl sulphosuccinate (SDOSS) as surfactant, isooctane and other chemicals were obtained from Wako, Japan.…”

Section: Methodsmentioning

confidence: 99%

CdS Nanoparticle-based Biosensor Development for Aflatoxin Determination

Hayat

Saepudin

Einaga

et al. 2019

IJTech

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An anodic stripping voltammetry detection method with lateral flow immunochromatographic assay for determining aflatoxin M1 was developed. Cadmium sulfide nanoparticles (CdSNPs) were employed as a label for aflatoxin antibody. Conjugation between the CdSNPs and aflatoxin antibody was performed as the first step. The CdSNP-antibody conjugate was then immobilized on a test strip on a conjugate pad. When the conjugate interacted with the sample containing aflatoxin, it was captured by the aflatoxin in the test zone. The CdSNPs retained in the test zone were then measured by anodic stripping voltammetry using a boron-doped diamond electrode. A linear concentration range of 0-70 ppb aflatoxin, with a sensitivity of 20 µA/mM and a detection limit of 30 ppb, was achieved by this method.

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Section: Methodsmentioning

confidence: 99%

CdS Nanoparticle-based Biosensor Development for Aflatoxin Determination

Hayat

Saepudin

Einaga

et al. 2019

IJTech

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“…Liu, et al [ 58 ] developed a simple and stable immunosensor based on CdS–Fe3O4 magnetic nanocomposites capable of detect the toxin at 5 pg/mL. Prihantoro, et al [ 71 ] recently purified an antibody to be used as a sensor for AFB 1 , but there is still a need to identify it.…”

Section: Perspectivesmentioning

confidence: 99%

A Glance at Aflatoxin Research in Mozambique

Cambaza

Koseki

Kawamura

2018

IJERPH

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In Mozambique, aflatoxin research started in the 1960’s and has been carried through apparently unrelated efforts according to opportunities. However, they can be grouped in two sets: early epidemiological studies and recent agricultural research. Early investigators found a strong correlation between aflatoxin contamination and primary liver cancer. Since then, there have been efforts to examine the extent of contamination, especially in groundnuts and maize. More recent investigations and interventions aimed mostly to reduce the level of contamination, enough to allow such commodities to gain acceptance in the international market. The current status of knowledge is still marginal but the increasing involvement of local authorities, academia, and international organizations seems promising.

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