Purification of Actinomycin D from Streptomyces parvulus Isolated from Mangrove Ecosystem of Kerala, India

Cibi, R.; Nair, A. Jayakumaran

doi:10.20546/ijcmas.2016.507.049

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…Many research publications have reported the isolation of Streptomyces parvulus species and its type strains indicating its frequency of isolation and their targeted screening for antibiotic production, such as production of actinomycin D by Streptomyces parvulus CBJ1 [34], anti Staphylococcus antibiotic by Streptomyces parvulus [35], and antimicrobial metabolites by Streptomyces parvulus DOSMB-D105 [36]. But their potential to produce other bioactive metabolites under submerged fermentation conditions has not been evaluated in a single study.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

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The significance and frequency of marine microorganisms as producers of bioactive metabolites-a natural source of drug discovery had varied significantly during the last decades, making marine ecosystem a huge treasure trove of novel isolates and novel compounds. Among the twelve actinomycetes isolated from marine sediment sample (Lat. 17°41′962″N, Long. 83°19′633″E), amylase, protease, lipase and cellulase activities were exhibited by 8,7,4,3 isolates respectively. Five isolates exhibited l-asparaginase activity, while 5, 6, 2 isolates exhibited antibacterial, antifungal and antimicrobial activities respectively. One isolate VMS-A10 efficiently producing alpha-amylase (25.53 ± 0.50 U/mL), protease (19.26 ± 0.25 U/mL), lipase (36.25 ± 0.10 U/mL), cellulase (14.43 ± 0.513 U/mL), l-asparaginase (0.125 ± 0.004 U/mL), antimicrobial metabolites against B. subtilis (503.33 ± 5.77 U/mL), S. aureus (536.66 ± 5.77 U/mL), E. coli (533.33 ± 5.77 U/mL), P. aeruginosa (500.00 ± 10.0 U/mL), MRSA (538.33 ± 5.77 U/mL), C. albicans (353.33 ± 11.54 U/mL) and A. niger (443.33 ± 15.27 U/mL) was selected, identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rDNA sequence, designated as Streptomyces parvulus strain sankarensis-A10 and sequencing product (1490 bp) was deposited in the GenBank database under accession number KT906299, Culture Deposit No: NCIM-5601. Isolation and characterization of each potential actinobacteria having immense industrial and therapeutic value on an unprecedented scale from marine sediments of Visakhapatnam coast will have a burgeoning effect.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

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“…Streptomyces is well-known as one genus of actinomycetes that produce many kinds of bioactive metabolite compounds. Streptomyces parvulus isolated from soil was reported as producer of peptide antibiotic actinomycin D (Inbar and Lapidot 1988) especially by S. parvulus CBJ1 (Cibi et al 2016) and antimicrobial metabolites by Streptomyces parvulus DOSMB-D105 (Baskaran et al 2016). Shaik et al (2017) also reported that species of S. parvulus, known as an important producers of polypeptide antibiotic actinomycins, was tolerant to various physiological conditions in the marine environment.…”

Section: Phylogenetic Analysis Of 16s Rdna Sequencesmentioning

confidence: 99%

Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia

et al. 2018

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Aeny TN, Prasetyo J, Suharjo R, Dirmawati SR, Efri, Niswati A. 2018. Isolation and Identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia. Biodiversitas 19: 2052-2058. This study was aimed to collect and identify actinomycetes from the rhizosphere of pineapple with the potency as a biocontrol agent of Dickeya zeae, the pathogen of pineapple soft rot. Soil samples were collected from four different pineapple plantations in Lampung Province, in December 2016. Samples were air-dried, serially diluted, and plated on modified ISP2 medium and several single characterized colonies were sub cultured and purified. The growing colonies were grouped into different colour series based on their aerial-mycelia and substrate-mycelia colors. Antagonistic activity of the actinomycetes isolates were evaluated against Dickeya zeae by agar diffusion method using the same medium agar lawn with the bacterial cultures. Selected antagonistic actinomycete isolates with the biggest clear zone formation were further characterized and identified molecularly based on 16S rDNA sequences. The results showed that as many as 34 actinomycete isolates collected from pineapple rhizosphere produced clear zones. Two isolates, namely GGF2-i5 and GGF4-i18, presented the biggest clear zone were proposed as the potential antagonist strains against D. zeae and identified as Streptomyces parvulus and Streptomyces hygroscopicus subsp. hygroscopicus, respectively. Both of the 16S rRNA gene sequences from those strains were deposited in the GenBank with the accession number MH170279 and MH170280, respectively.

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“…The assay does not require specialized equipment. Cibi and Jayakumaran, (2016) added that the method does not require animal serum as is the case with other cytotoxic assays.…”

Section: Introductionmentioning

confidence: 99%

Purification and cytotoxic assays of four antimicrobial metabolites extracted from Actinomycetes of the soils of Menengai Crater, Kenya

P.N.

F.B.

J.M.

et al. 2019

Novel Research in Microbiology Journal

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The search for antibiotic metabolites is increasing due to drug resistance been witnessed today. This study was conceived to isolate, identify and compare the antimicrobial metabolites yield of four actinomycetes isolated from Menengai crater soil, Kenya. The actinomycetes were isolated using starch casein, Luria Bertani (M1) and starch nitrate agar. The antimicrobial metabolites were extracted using standard techniques followed by purification, test for antagonism against selected pathogenic microbes, and cytotoxicity assay using Brine shrimp lethality test. Totally, 138 actinomycetes isolates were obtained from all the soil samples. Four isolates showed the highest potent potential against selected bacterial and fungal pathogens. The selected actinomycetes were coded; PAN 30, PAN 37, PAN 41, and PAN 154, and preserved at 4°C for further analysis. The difference in yield of the antimicrobial metabolites between liquid and solid-state fermentation was statistically significant (P= 0.005). Zones of inhibition did not vary significantly (F = 6.6046, P = 0.001338). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of the antimicrobial metabolites extracted from PAN 30, PAN 37, PAN 41 and PAN 154 isolates were equal. The Half Lethal Concentration (LC 50 ) in (µg/ ml) for the antimicrobial metabolites extracted from the 4 isolates were; PAN 30 (1.8168 ± 0.47), PAN 37 (3.4269 ± 0.56), PAN 41 (3.4269 ± 0.45), and PAN 154 (4.9397 ± 0.41). The four actinomycetes produced bioactive metabolites. Solid-state fermentation was superior to liquid-state fermentation in recovering the antimicrobial metabolites from the actinomycetes isolates. Molecular identification of the selected isolates needs to be carried out in a future study. Moreover, there is a need to determine the structure of the antimicrobials using nuclear magnetic resonance.

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Purification of Actinomycin D from Streptomyces parvulus Isolated from Mangrove Ecosystem of Kerala, India

Cited by 8 publications

References 9 publications

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia

Purification and cytotoxic assays of four antimicrobial metabolites extracted from Actinomycetes of the soils of Menengai Crater, Kenya

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