Purification of a Toxic Phytohæmagglutinin from Black Beans (Phaseolus vulgaris)

Jaffé, W. G.; Gaede, K.

doi:10.1038/1831329a0

Cited by 37 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The poor nutritional quality of kidney beans has been variously ascribed to the anti-nutritional effect of their lectin components (Jaffe and Gaede 1959;Pusztai et a1 1981) and to an overall deficiency in the sulphurcontaining amino acid content of the constituent proteins (Evans and Bauer 1978). Thus growing pigs consuming a Phaseolus vulgaris cultivar of high lectin content suffered a drastic loss of weight (King et a1 1983); nevertheless, when a low-lectin cultivar was substituted, only a moderate improvement in growth rate resulted (Begbie and King 1985).…”

Section: Discussionmentioning

confidence: 99%

Resistance of the kidney bean reserve protein, phaseolin, to proteolysis in the porcine digestive tract

Begbie¹,

Ross²

1993

J Sci Food Agric

View full text Add to dashboard Cite

Phaseolin, the major reserve protein of the seeds of the kidney bean, Phaseolus vulgaris L, was shown to be poorly degraded in the pig small intestine following an initial 7-day exposure to casein-based diets incorporating kidney bean meal. Substantial amounts of large polypeptide fragments, which were recognised by antibodies raised against native phaseolin, were identified in ileal digesta. Little of this immunoreactivity was detected in the faeces of these animals, suggesting that components of the hindgut microflora were able to degrade these fragments further. Prolonged exposure to dietary kidney beans resulted in an increase in the digestibility of phaseolin, suggesting that the small intestine and/or its microflora may possess significant adaptive capacity. In-vitro digestion of purified phaseolin by a sequence of pepsin, trypsin and chymotrypsin yielded products which closely resembled those isolated from the ileal digesta; analysis suggested that degradation of native phaseolin by the major gastrointestinal endopeptidases is restricted to a vulnerable central region of its constituent polypeptides.

show abstract

Section: Discussionmentioning

confidence: 99%

Resistance of the kidney bean reserve protein, phaseolin, to proteolysis in the porcine digestive tract

Begbie¹,

Ross²

1993

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…These workers considered their preparation as possibly being identical with that of Jaffé et al [17]. The toxic in vivo action in rats is exerted mainly on lymphoid organs which are involuted, leading to changes in the myelo-lymphoblastic ratio in the circulating, splenic and marrow blood which finally results in death of the animal.…”

Section: Miscellaneous Toxic Proteins In Seedsmentioning

confidence: 86%

“…Camejo [9] reported 128,000 for the molecular weight of phaseolotoxin A, one of the hemagglutinins of the black kidney bean isolated by the procedure of Jaffé and Gaede [17]. By careful repeated fractionation with ammonium sulfate Jaffé and Hannig [16] obtained a preparation of the hemagglutinin which was homogeneous by paper and free-flow electrophoresis and had an isoelectric point of pH 4.9.…”

Section: Hemagglutinins From Leguminosaementioning

confidence: 99%

Phytohemagglutinine - A Survey of Recent Progress

Dechary¹

1968

Vox Sanguinis

View full text Add to dashboard Cite

This paper is a review of recent articles by other investigators on phytohemagglutinins and is by necessity selective in nature. Previously known hemagglutinins of members of the family Leguminosae (Phaseolus, Soja) have been purified and some of their physical and chemical properties determined. The mitosis-stimulating factor of P. vulgaris has been purified and the hemagglutinating component separated from it. Another member of Leguminosae (Wistaria) has been found to possess mitogenic and blastogénie properties and the pokeweed mitogen has been isolated and characterized. Concanavalin A, the nonspecific hemagglutinin of jack bean, has been highly purified and its quaternary structure determined. The determination of stereochemical requirements for inhibition studies with hemagglutinins now provides a new tool for the solution of unresolved problems in carbohydrate identification and structure. Toxic proteins have been isolated from several members of the family Euphorbiacae. Ricin B, the toxic, nonhemagglutinating protein of the castor bean has been highly purified and some of its physical properties determined.

show abstract

“…This suggests that toxicity and agglutination are two distinct and separate activities, a suggestion which was first made by Evans (1965a, b, 1966). Jaffe and Gaede (1959) document the striking difference between two fractions extracted from the red kidney bean, viz. phytotoxin A and a fraction which he called B.…”

Section: Discussionmentioning

confidence: 98%

The Effect of the Consumption of Red Kidney Beans (Phaseolus vulgaris) on the Growth of Rats and the Implications for Human Populations

McPherson

1990

Journal of the Royal Society of Health

View full text Add to dashboard Cite

Raw Red Kidney beans (Phaseolus vulgaris) at a level as low as 1% of diet have been shown to cause death in rats in two weeks. Beans cooked at 100 degrees for 30 mins, and incorporated at a level as high as 20% of diet do not retard growth when tested against casein. However, when beans which have cooked at 70 degrees for 30 mins are incorporated growth retardation is almost as great as that which occurs when raw beans are fed. The small amount of lectin present in beans cooked at 70 degrees might be responsible for this effect.

show abstract

Purification of a Toxic Phytohæmagglutinin from Black Beans (Phaseolus vulgaris)

Cited by 37 publications

References 5 publications

Resistance of the kidney bean reserve protein, phaseolin, to proteolysis in the porcine digestive tract

Resistance of the kidney bean reserve protein, phaseolin, to proteolysis in the porcine digestive tract

Phytohemagglutinine - A Survey of Recent Progress

The Effect of the Consumption of Red Kidney Beans (Phaseolus vulgaris) on the Growth of Rats and the Implications for Human Populations

Contact Info

Product

Resources

About