Purification of a Thermostable Lipase Geobacillus thermodenitrificans nr68 (Lip.nr-68) with Potential for Enzymatic Deinking

Him, Nik Raikhan Nik

doi:10.18178/jolst.4.2.70-74

Cited by 2 publications

(2 citation statements)

References 10 publications

(10 reference statements)

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“…Lipase from G. thermodenitrificans nr68 has expressed great enzymatic biodeinking activity toward a laser jet printed paper, with deinkability brightness test of 55%, a value that was slightly lower than that shown by a commercial lipase (63%). Such lipases can be used in the recycling of waste paper [94]. Geobacillus sp.…”

Section: Production and Applications Of Thermostable Enzymes Of Geobamentioning

confidence: 99%

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

Новик¹,

Savich²,

Meerovskaya³

2019

Growing and Handling of Bacterial Cultures

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The genus Geobacillus is represented by obligately thermophilic bacteria able to grow in the temperature range of 35-75°C. They are modest bacteria isolated from various sources on routine media such as nutrient agar. Originally classified as representatives of Bacillus, the species of Geobacillus were established in 2001 as a new genus. However, sequence similarity between all species indicates that at least some species need to be reclassified at the genus level. In addition to 16S rRNA, housekeeping genes, 16S-23S rRNA gene internal transcribed spacer, and repetitive sequences can be used in classification and identification of thermophilic bacteria. The ability to survive and grow at high temperatures as well as utilization and synthesis of a wide range of compounds makes these bacteria and their products attractive for use in various spheres: food, paper, biotechnology industries, medicine, bioremediation, etc. A broad spectrum of applications arouses increased interest in the study of physiological and biochemical characteristics and triggers emergence of new usage areas for Geobacillus, such as bioenergy. The growing demand for energy leads to the development of alternative technologic options. Geobacillus species demonstrated the ability to generate or enhance productivity of important sources of bioenergy such as ethanol, isobutanol, 2,3-butanediol, biodiesel, and biogas.

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Section: Production and Applications Of Thermostable Enzymes Of Geobamentioning

confidence: 99%

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

Новик¹,

Savich²,

Meerovskaya³

2019

Growing and Handling of Bacterial Cultures

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“…This finding is intriguing because Geobacilli are predominantly Gram-positive. However, few thermophilic Geobacilli have been reported to be Gram-positive [16,17]. It has been suggested that hyperthermophilic archaea and bacteria are anciently related and both slowly evolve following different paths.…”

Section: Genotypic Identification Of Strain Pls Amentioning

confidence: 99%

Purification and partial characterization of a thermo-halostable protease produced by Geobacillus sp. strain PLS A isolated from undersea fumaroles

Iqbalsyah

Malahayati

Atikah

et al. 2019

Journal of Taibah University for Science

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The growing industrial demand for stable proteases has driven this study to purify and partially characterize a protease from a bacterial strain isolated from undersea fumaroles. Phylogenetic analysis of the 400bp conserved area of the 16S rRNA gene indicated that the isolate was related to Geobacillus thermoleovorans strain SGAir 0734. Purification by ammonium sulfate precipitation and anion exchange chromatography produced 118-fold purity. The optimum activity (646 U mL −1 ) of the pure enzyme was observed at 60°C, pH 7 and with 5M sodium chloride addition. The enzyme retained 73% of its activity when in 50% n-hexane, while the activity was only 40% of the control when in 50% ethyl acetate. The enzyme was tested for its leather dehairing capability. It only caused a slight dehairing and produced soft leather. The enzyme may be applied in skin rejuvenation, wrinkle-smoothing, and high-quality suede production industries. ARTICLE HISTORY

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Purification of a Thermostable Lipase Geobacillus thermodenitrificans nr68 (Lip.nr-68) with Potential for Enzymatic Deinking

Cited by 2 publications

References 10 publications

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production

Purification and partial characterization of a thermo-halostable protease produced by Geobacillus sp. strain PLS A isolated from undersea fumaroles

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