Purification of a Tasmanian isolate of subterranean clover red leaf virus, and its serological interactions with a New Zealand isolate and other luteoviruses

Johnstone, G. R.; Duffus, J. E.; Munro, D. C.; Ashby, J. W.

doi:10.1071/ar9820697

Cited by 14 publications

(6 citation statements)

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“…The two isolates of SCRLV are the most closely related and quite distinct form both BLRV and BWYV. These latter two viruses are also clearly distinct from each other, though all have some serological relationship to one another and to other members of the luteovirus group (Ashby and Kyriakou, 1982;Rochow and Duffus, 1981;Johnstone et al, 1982).…”

Section: Discussionmentioning

confidence: 96%

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The host ranges, classification and identification of eight persistent aphid-transmitted viruses causing diseases in legumes

Johnstone¹,

Ashby²,

Gibbs

et al. 1984

Netherlands Journal of Plant Pathology

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Seventy one seed-lines representing 23 species of papilionoid legumes and 17 species of nonlegumes were collected and distributed to four countries; Australia, New Zealand, the Netherlands and the U.S.A. In each of these countries plants were grown from the seeds and their suscept~ility to a total of eight persistent aphid isolates transmitted viruses was assessed. The viruses were a strain of beet western yellows (BWYV) from Glycine maxin Illinois, legume yellows (LYV) in California and a virus in Michigan (MiAV) from Medicago sativa, from Pisum sativum causing leaf roll and top yellows in New Zealand (PeLRV-NZ) and the Netherlands (BLRV), isolates of subterranean clover red leaf from New Zealand (SCRLV-NZ) and Tasmania (SCRLV-T); and Subterranean clover stunt (SCSV) from Tasmania.The relationships between the eight viruses as indicated by their host reactions were assessed using computer classification techniques. SCRLV-NZ and SCRLV-T were the most similar. They had moderately wide host ranges that included some non-legumes. A second group comprized BWYV and PeLRV-NZ. These were typical of most beet western yellows virus strains in that they infected Brassica napus, Capsella bursa-pastoris and Stellaria media. MiAV and BLRV also formed a pair. They generally induced severe symptoms on the hosts which they infected and had host ranges confined to legumes except that BLRV also infected Claytonia perfoliata and Erodium spp. The relationships of LYV and SCSV were not consistent. They paired together in some classifications, but SCSV sometimes grouped with the SCRLV isolates. Both had host ranges confined to legumes, caused severe symptoms in most hosts and were often difficult to recover from affected plants. LYV had some affinities with BLRV and MiAV.The tests indicated a set of test plants which were most useful for propagating and identifying persistent aphid-transmitted viruses from legumes. Two, P. sativum cv. Onyx and Trifolium subterraneum cv. Bacchus Marsh were susceptible to all isolates. Ten others distinguished between t]he isolates and were Arachis hypogea, Beta vulgaris, C. bursa-pastoris, G. max cv.

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Section: Discussionmentioning

confidence: 96%

“…Since then the particles of an additional four of the isolates have been purified, visualized and at least partially characterized, namely BLRV (Ashby and Huttinga, 1979), LYV (Duffus, 1979) and the two isolates of SCRLV (Ashby and Kyriakou, 1982;Johnstone et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

The host ranges, classification and identification of eight persistent aphid-transmitted viruses causing diseases in legumes

Johnstone¹,

Ashby²,

Gibbs

et al. 1984

Netherlands Journal of Plant Pathology

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“…This paper reports the purification and characterisation of SCRL V and its serological relationship to some luteoviruses. Further studies on the purification of SCRL V and the serological reactions of Tasmanian and New Zealand isolates with antisera to various luteoviruses are reported by Johnstone et al (1982).…”

Section: Introductionmentioning

confidence: 99%

Purification and properties of subterranean clover red leaf virus

Ashby¹,

Kyriakou

1982

New Zealand Journal of Agricultural Research

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Subterranean clover red leaf virus (SCRL V) was purified from infected peas using cellulase to assist in virus extraction. Yields of 0.5-3.0 mg/kg tissue were obtained. The virions were isometric with a diam. of 27 nm and sedimented as a single component of 114 S. The mean A260/ A280 of purified preparations was 1.85. Apterae of Aulaeorthum solani (Kltb.) when fed on purified preparations through parafilm membranes were able to acquire and transmit virus. Purified virus had a dilution end point of 1.36 /-Ig/ml, a thermal inactivation point between 55 and 60°C, and longevity in vitro of greater than 14 days.

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“…Based on the persistence of its transmission by the aphid Aulacorthum solani (Kltb. ), the nature of its symptoms in legumes (Kellock, 1971;Johnstone, 1978;Ashby et al, 1979), the association of luteovirus-like particles with infection (Jayasena et al, 1981) and its serological relationship with other luteoviruses (Johnstone et al, 1982;Ashby & Kyriakou, 1982) SCRLV has been assigned to the luteovirus group (Matthews, 1982).…”

Section: Introductionmentioning

confidence: 99%

“…Before the development of serological methods for viruses in this group (Aapola & Rochow, 1968;Roberts et al, 1980;Johnstone et al, 1982;Ashby & Kyriakou, 1982), identification was based on transmissibility by specific aphid vectors and symptomatology.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a Complementary DNA Probe Specific for Detecting Subterranean Clover Red Leaf Virus in Plants and Aphids

Jayasena

Randles

Barnett

1984

Journal of General Virology

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SUMMARYTwo RNA species, and one DNA species, were isolated from subterranean clover red leaf virus (SCRLV) prepared by a modification of previously described methods. 3H-labelled cDNA transcribed from high molecular weight RNA of purified virus was specific for the detection of SCRLV, in that it showed no hybridization with nucleic acids from either healthy plants, or plants infected with the serologically related potato leaf roll virus, but hybridized with homologous RNA and nucleic acids from SCRLVinfected plants of two species. The cDNA detected SCRLV in individuals and groups of the aphid vector, Aulacorthum solani, and the average virus content was greater than 170 pg per aphid. The non-vector, Myzus persicae, contained only trace amounts of SCRLV, a result confirmed by enzyme-linked immunosorbent assay.

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Purification of a Tasmanian isolate of subterranean clover red leaf virus, and its serological interactions with a New Zealand isolate and other luteoviruses

Cited by 14 publications

References 0 publications

The host ranges, classification and identification of eight persistent aphid-transmitted viruses causing diseases in legumes

The host ranges, classification and identification of eight persistent aphid-transmitted viruses causing diseases in legumes

Purification and properties of subterranean clover red leaf virus

Synthesis of a Complementary DNA Probe Specific for Detecting Subterranean Clover Red Leaf Virus in Plants and Aphids

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