Purification of a specific inhibitor of pyroglutamyl aminopeptidase II from the marine annelide Hermodice carunculata

Tri-domain Bifunctional Inhibitor of Metallocarboxypeptidases A and Serine Proteases Isolated from Marine Annelid Sabellastarte magnifica

“…Several such inhibitors capable of independently inhibiting proteases of different mechanistic classes have been described, particularly in the phyla Cnidaria (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27), Mollusca (28 -31), and Annelida (32,33). However, no inhibitors of CPs of this type had been described in such sources until the present.…”

Screening of Protease Inhibitory Activity in Aqueous Extracts of Marine Invertebrates from Cuban Coast

“…For most of the extracts the protein concentration decreased by almost two times after the heating treatment, however the total inhibitory activity did not change, so the specific inhibitory activity also increased by almost two times. Moreover, many already identified protease inhibitors from marine invertebrates are low molecular weight proteins, resistant to high temperatures, like the assayed in this work [23] [25] [26] [28]. The highest number of positive species (half of the extracts analyzed) were detected when analyzing inhibitory activity against PPE.…”

“…Many compounds with therapeutic potentialities have been isolated from these organisms, although most of them are poorly studied [17]. Cuban coasts have a remarkable variety of marine invertebrates, which allows screening of biological activity straight from natural sources as a first step to identify new molecules with potential properties such as toxins, antimicrobial peptides, proteases and inhibitors [23]- [28]. The data presented here describe systematic screening experiments to identify inhibitory activities applying twenty aqueous extracts of selected marine invertebrates against four proteases of therapeutic relevance.…”

Biologically active compounds from natural and marine natural organisms with antituberculosis, antimalarial, leishmaniasis, trypanosomiasis, anthelmintic, antibacterial, antifungal, antiprotozoal, and antiviral activities

“…These natural compounds affecting the cardiovascular, immune and nervous systems, as well anti-inflammatory effects (Pascual et al, 2004), antimicrobial activity in sub-Arctic marine invertebrates (Lippert et al, 2003), antifilarial activity of the red alga Botryocladia leptopoda (Lakshmi et al, 2004), antiviral effects of a sulfated exopolysaccharide from the marine microalga Gyrodinium impudicum (Yim et al, 2004) and Sargassuum patens (Zhu et al, 2004), a polyhydroxylated fucophlorethol isolated from the marine brown alga Fucus vesiculosus shown to be bactericidal towards selected Grampositive and Gram-negative bacteria in vitro (Sandsdalen et al, 2003); and an improvement of "current cytokine-based therapies" by sulphated polysaccharides purified from the green alga Codium fragile, as well as fucoidan and carrageenan, isolated from brown and red algae, respectively (Nika et al, 2003). The bioactivities or pharmacology of natural products have been established (Mayer and Lehmann, 2000Schmitz et al, 1993).…”