Purification of a secreted lectin from Andrias davidianus skin and its antibacterial activity

Qu, Min; Tong, Changqing; Kong, Liang; Yan, Xin; Chernikov, Oleg V.; Lukyanov, Pavel; Jin, Qiao; Li, Wei

doi:10.1016/j.cbpc.2014.08.008

Cited by 9 publications

(11 citation statements)

References 30 publications

(23 reference statements)

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“…The lectin from Andrias davidianus (ADL) showed effects on the respiratory chain and energy production of bacterial cells, changing respiratory metabolism and affecting tricarboxylic acid cycle and hexose monophosphate pathway (Qu et al . ).…”

Section: In Vitro and In Vivo Antimicrobial Evaluation Of Lectinsmentioning

confidence: 97%

Lectins as antimicrobial agents

et al. 2018

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Summary The resistance of micro‐organisms to antimicrobial agents has been a challenge to treat animal and human infections, and for environmental control. Lectins are natural proteins and some are potent antimicrobials through binding to carbohydrates on microbial surfaces. Oligomerization state of lectins can influence their biological activity and maximum binding capacity; the association among lectin polypeptide chains can alter the carbohydrate–lectin binding dissociation rate constants. Antimicrobial mechanisms of lectins include the pore formation ability, followed by changes in the cell permeability and latter, indicates interactions with the bacterial cell wall components. In addition, the antifungal activity of lectins is associated with the chitin‐binding property, resulting in the disintegration of the cell wall or the arrest of de novo synthesis from the cell wall during fungal development or division. Quorum sensing is a cell‐to‐cell communication process that allows interspecies and interkingdom signalling which coordinate virulence genes; antiquorum‐sensing therapies are described for animal and plant lectins. This review article, among other approaches, evaluates lectins as antimicrobials.

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Section: In Vitro and In Vivo Antimicrobial Evaluation Of Lectinsmentioning

confidence: 97%

Lectins as antimicrobial agents

et al. 2018

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“…The respiration inhibition test was carried out according to previous studies with modifications (Guo et al 2005 ; Ma et al 2010 ; Qu et al 2015 ). A mixture containing 1.6 mL of 1% glucose, 14.4 mL of PBS and 4 mL of E. coli CFT073 bacterial suspension in the logarithmic growth phase was stirred lightly for 5 min in a reagent bottle sealed with a preservative film.…”

Section: Methodsmentioning

confidence: 99%

“…These results suggest DM inhibits fungal and bacterial respiration via a different mechanism. Iodine acetic acid, malonic acid and sodium phosphate is the typical respiratory inhibitor of the EMP pathway, TCA pathway and HMP pathway, respectively (Guo et al 2005;Ma et al 2010;Qu et al 2015). If two inhibitors block the same pathway, the superposition of the inhibitory rate is weak.…”

Section: The Inhibitory Ability Of Dm Against the Tested E Coli Strainsmentioning

confidence: 99%

Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli

Chen

Qin

et al. 2020

AMB Expr

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This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.

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“…Antimicrobial peptide from mucus of andrias davidianus was found to be inhibitory both to gram-negative and gram-positive bacteria. Furthermore, relevant toxicological experiments [8][9][10] also show that the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use purified a lectin secreted from andrias davidianus skin (ADL) and significant the ADL activity between pH 5-8. The experimental results show that the activity was stable between 20 and 50°C, and the lectin reaction did not depend on the presence of the divalent cation Ca 2+ or Mg 2+ .…”

Section: Introductionmentioning

confidence: 99%

Composition and pharmacological analysis of loach mucus

Hao²,

Zhuo³

et al. 2022

J. Phys.: Conf. Ser.

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Hydrogel plays an important role in the composition of biological tissue. Muscle, cell membrane, blood vessel wall, hair and cartilage in human body can be regarded as hydrogel. The mucus on the surface of natural living organisms such as loach, andrias davidianus and earthworm is natural hydrogel. This kind of mucus with peculiar smell is produced by living organisms due to external stimulation of body surface stress response. It is a kind of self-protection. And this mucus has certain effects of antibacterial, antifatigue and enhancing immunity. The main component of mucus is collagen with shorter molecular structure and molecular weight is relatively small, and the content of collagen can reach about 40 ∼ 60 % in mucus. It has a certain bonding effect on skin surface damage, and plays the function of quickly closing wounds and killing bacteria. Hyaluronic acid (HA), active structure in oligosaccharide peptide and collagen peptide extracted from mucus, particularly type II collagen, play important roles in skin repair. This function of this natural hydrogel may open a new approach for the development of secondary functions of such organisms.

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Purification of a secreted lectin from Andrias davidianus skin and its antibacterial activity

Cited by 9 publications

References 30 publications

Lectins as antimicrobial agents

Lectins as antimicrobial agents

Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli

Composition and pharmacological analysis of loach mucus

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