Purification of a recombinant Schistosoma japonicum antigen homologous to the 22-kDa membrane-associated antigen of S. mansoni, a putative vaccine candidate against schistosomiasis

Effect of Ultra Violet Attenuated Cercariae on Antibody Response in Rabbits

“…Identified cDNA clone, UVISmC1 encoding an antigen that showed 100% identity at the amino acid level with previously identified S. mansoni clones, encoding 51.7 kDa antigens, isolated from S. mansoni sporocyst and 25 days schistosomula cDNA expression libraries (Francis andBickle, 1992, Ahmed et al, 2001). However, this Ag showed a lower degree of identity with other previous studies identifing S. mansoni and S. japonicum clones, and encoding 22.6 kDa antigens, isolated from S. mansoni and S. japonicum adult worm expression library (Stein and David, 1986, Jeffs et al, 1991, Waine et al, 1994, Yang et al, 1997. Therefore, further studies to examine some genes clone encoding antigens isolated from UV irradiated S. mansoni expression library in order to engineer vaccine candidates are still needed.…”

Molecular Characterization of a 20.8-kDaSchistosoma mansoni Antigen

“…Sm 20.8 is also highly similar to the members of a family of tegumental associated antigens previously described (11)(12)(13)(14) (Fig. 4C).…”

“…The sequence encoding this antigen (Sm 20.8) is similar to those of the 8-kDa family of DLCs (of which SmDLC is a member), an 18-kDa calcium-binding outer arm DLC from Chlamydomonas reinhardtii (10), and a family of schistosome proteins termed tegumental associated antigens (11)(12)(13)(14)). Members of this family all (i) have conservative calcium binding EF hand-like motifs, but none (including Sm 20.8) binds calcium experimentally; (ii) display developmentally regulated expression; and (iii) have as of yet no identified functions or significant sequence homologies.…”

“…These scores indicate the similarity of Sm 20.8 to a group of previously characterized polypeptides from schistosomes termed tegumental associated antigens (11)(12)(13)(14). Comparison of the alignment between Sm 20.8 and this group of proteins (Fig.…”

FhCaBP1 (FH22): A Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

“…This species expresses 13 family members, some of which have been shown to elicit IgE-mediated immune responses in the host (Fitzsimmons, et al, 2012, Fitzsimmons, et al, 2007, Webster, et al, 1997. Proteins from this family have also been identified and characterised from other species in the genus Schistosoma, from the Chinese liver fluke Clonorchis sinensis (Huang, et al, 2007, Kim, et al, 2012, Senawong, et al, 2012, Subpipattana, et al, 2012, Vichasri-Grams, et al, 2006, the giant liver fluke Fasciola gigantica and the carcinogenic liver fluke, Opisthorchis viverrini (Fitzsimmons, et al, 2004, Li, et al, 2000, Santiago, et al, 1998, Waine, et al, 1994, Xu, et al, 2014, Zhang, et al, 2012.…”