Purification of a protein from coelomic fluid of the earthworm Eisenia foetida and evaluation of its hemolytic, antibacterial, and antitumor activities

Zhang, Hua; Wang, Yanhong; Cao, Hongwei; Liao, Pu; Cui, Yudong

doi:10.3109/13880209.2010.508498

Cited by 26 publications

(17 citation statements)

References 20 publications

(21 reference statements)

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“…These invertebrates can be considered as one of the rich sources of biologically and pharmacologically active compounds for use in the treatment of various diseases, including cancers (5,6). Earthworm coelomic fluid and extracts showed antibacterial and antifungal activities (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) as well as antiviral action (18). Earthworm tissues, extracts, or isolated fractions and compounds exhibited anticancer activity toward different cancer cell lines (19)(20)(21)(22)(23)(24)(25)(26).…”

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confidence: 99%

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Fiołka¹,

Rzymowska

Bilska

et al. 2019

APMIS

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It is known that earthworm coelomic fluid (CF) can affect not only cancer but also normal cells. The study demonstrated that the CF of the earthworm Dendrobaena veneta exhibited cytotoxicity against A549 lung cancer cells but did not toward the bronchial epithelial cell line BEAS‐2B. The selective effect on the tumor cells was achieved after a short‐term CF heat pre‐treatment at 70 °C. The cytotoxic effect of the CF was time‐ and concentration‐dependent. The CF noticeably decreased the viability and affected the morphology of the A549 cells. Scanning electron microscopy revealed a different degree of destruction of the nucleus and cytoplasm of A549 cells. As determined by atomic force microscopy, the cell surface roughness increased while the cell stiffness was reduced upon the CF treatment. A twofold increase in the caspase 3, 4, 5, and 10 levels was observed in the A549 cells after the incubation with the CF. The results obtained by flow cytometry using Annexin V confirmed the proapoptotic effect of the earthworm CF on A549 lung cancer cells. The D. veneta CF and active fraction obtained with cytotoxicity toward A549 lung cancer is an interesting and promising preparation for further biological, chemical, and biomedical research.

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confidence: 99%

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Fiołka¹,

Rzymowska

Bilska

et al. 2019

APMIS

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“…Earthworm proteases could directly kill the cancer cells in vitro [22]. Earthworm proteases also enhanced the curative effects by both radiation therapy and chemotherapy [23].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression, and anti-tumor activity of a novel serine protease from <italic>Arenicola cristata</italic>

Zhao

2014

ABBS

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Arenicola cristata, a marine annelid, is a well-known and prized traditional Chinese medicine. However, the serine protease gene of A. cristata has not been cloned yet. In this study, a novel protease of A. cristata was cloned, sequenced, and expressed in Escherichia coli, and the functions of this recombinant protease were also investigated. The whole complementary DNA (cDNA) of this novel protease was of 980 bp in length and consisted of an open reading frame of 861 bp encoding 286 aa. Sequence analysis of the deduced amino acid sequence revealed that the protease belongs to the serine protease family. The active enzyme of the proposed A. cristata protease is composed of a signal peptide, a propeptide, and a mature polypeptide. The molecular weight of the recombinant mature protein was ∼26 kDa after over-expression in E. coli. The recombinant protein significantly inhibited cell growth and induced cell apoptosis of esophageal squamous cell carcinoma (ESCC) in vitro, and reduced tumorigenicity in vivo. Furthermore, administration of the recombinant protein led to the activation of caspase-9 as well as down-regulation of Mcl-1 and Bcl-2. Taken together, our findings indicated that the recombinant serine protease of A. cristata could inhibit ESCC cell growth by mitochondrial apoptotic pathway and might act as a potential pharmacological agent for ESCC therapy.

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“…(Liu, 1983;Liang, 1984;Mu, 1988;Kurek et al, 2007;Paul, 2014). Several authors have also reported earthworms to have antimicrobial properties (Hua et al, 2011;Hossam et al, 2012;Mathur et al, 2010;Chauhan et al, 2014;Esavaniet al, 2017). This is an important property as today development of microbial resistance to various existing antimicrobial drugs has become a serious public health concern (Esavaniet al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity of Earthworm Lampitomauritii Paste against Bacteria and Fungi

Senthil¹,

Sivakami²

2018

Int.J.Curr.Res.Aca.Rev.

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Article InfoEarthworms lives in the soil along with other microorganisms, some of which can cause diseases to main and other organisms. This has made these worms to develop defence mechanisms against these organisms. It has been reported that earthworms can cure a number of diseases. This has prompted studies on using various species of earthworms to test their antimicrobial activities. Hence, the present study was attempted to study the antimicrobial activity of Lampitomauritii against bacterial and fungal organisms which was collected from Musiri area. Results indicate that among bacteria the highest inhibition was shown by Escherichia coli and the least by Vibrio cholerae and among fungi, the highest inhibition was shown by Penicillium citrinum and least by Candida albicans.

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Purification of a protein from coelomic fluid of the earthworm Eisenia foetida and evaluation of its hemolytic, antibacterial, and antitumor activities

Cited by 26 publications

References 20 publications

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Molecular cloning, expression, and anti-tumor activity of a novel serine protease from <italic>Arenicola cristata</italic>

Evaluation of Antimicrobial Activity of Earthworm Lampitomauritii Paste against Bacteria and Fungi

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Purification of a protein from coelomic fluid of the earthworm Eisenia foetida and evaluation of its hemolytic, antibacterial, and antitumor activities

Cited by 26 publications

References 20 publications

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Molecular cloning, expression, and anti-tumor activity of a novel serine protease from &lt;italic&gt;Arenicola cristata&lt;/italic&gt;

Evaluation of Antimicrobial Activity of Earthworm Lampitomauritii Paste against Bacteria and Fungi

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Molecular cloning, expression, and anti-tumor activity of a novel serine protease from <italic>Arenicola cristata</italic>