Purification of a new galactanase from <i>Penicillium oxalicum</i> catalysing the hydrolysis of <i>β</i>-(1→5)-galactofuran linkages

Reyes, Fuensanta; Alfonso, Carlos; Martı́nez, Marı́a Jesús; Prieto, Alicia; Santamaría, F.; Leal, J.A.

doi:10.1042/bj2810657

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…Enzymatic hydrolysis of 1(1-5)-linked galactofuranosyl residues from EPS of P. charlesii by an exo-13-D-galactofuranosidase produced by this fungus was reported (26). An endo-13(1-5)-D-galactofuranosidase from P. oxalicum and the enzymatic hydrolysis of 13(1-5)-galactofuranosyl residues from polysaccharides obtained from Eupenicillium crustaceum and Talaromyces flavus have also been reported (25). However, the influence of the exo-13-D-galactofuranosidase on the antigenic properties of EPS has not been studied.…”

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confidence: 99%

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

et al. 1992

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To study the structures of the epitopes of the extracellular polysaccharides from Penicilium and Aspergillus species, an exo-13-D-galactofuranosidase was purified from a commercial crude enzyme preparation from Trichoderma harzianum. Analysis of ring size and linkage position of the galactose residues of the extracellular polysaccharide of Penicillium digitatum, before and after enzymatic treatment, was determined by the reductive-cleavage technique. In addition to terminal and 13(1-5)-linked galactofuranosides, P(1-6)-linked and P(1,5,6)-linked branched galactofuranose residues could be identified. After degradation with the purified exo-13-D-galactofuranosidase, all initial linkages of the galactofuranose residues were still present, but the amount of D(1-5)-linked galactofuranose residues had decreased considerably. Treatment of the extracellular polysaccharides ofPenicillium and Aspergils species with the purified exo-o-D-galactofuranosidase resulted in complete disappearance of the enzyme-linked immunosorbent assay reactivity of these polysaccharides, using immunoglobulin G antibodies raised against P. digitatum. Therefore, with the use of this enzyme, it was proved that the 13(1-5)-linked galactofuranosyl residues only are responsible for the antigenicity of the extracellular polysaccharides ofPenicillium and Aspergills molds. A new structural model for the antigenic galactofuranose side chains of the galactomannan from P. digitatum is proposed. Molds are able to excrete a large variety of polysaccharides, some of them with antigenic properties (22, 29). Several authors studied the chemical structure of polysaccharides isolated from mycelium of mold species belonging to the genera Penicillium and Aspergillus (3-5). Penicillium and Aspergillus species are important fungi which are distributed worldwide and are the cause of many cases of food spoilage. Medically, some members are significant because of their ability to cause aspergillosis in humans and the release of mycotoxins. The polysaccharides are constituted mainly of mannose, galactose, and glucose, with minor amounts of protein (3-5). Galactosamine has been found in the polysaccharides of several Aspergillus species (2, 13, 27). Extracellular polysaccharides (EPSs) produced by these molds appeared to have similar structures (12, 24).

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confidence: 99%

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

et al. 1992

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“…Cell wall-degrading enzymes, such as chitinase and glucanase (Mavingui and Heulin 1994;Nielsen and Sorensen 1997), are capable of distorting hyphal cells and egg shells. Therefore, chitinase could also play a role in causing nematode mortality because it has been reported that P. oxalicum (ATCC number 201888) also produces chitinase (Rodriguez et al 1993), galactanase (Reyes et al 1992) and b-N-acetylglucosaminidase . It has also been reported that the chitinase from Paenibacillus illinoisis KJA-424 causes lysis of the Meloidogyne incognita eggshell, as well as inhibiting juveniles hatching and causing abnormal swelling and deformation of Rhizoctonia solani hyphae (Jung et al 2002(Jung et al , 2003.…”

Section: Discussionmentioning

confidence: 99%

Penicillium oxalicum reduces the number of cysts and juveniles of potato cyst nematodes

Martinez-Beringola¹,

Salto²,

Vázquez³

et al. 2013

J Appl Microbiol

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Aim: To test the biocontrol potential of Penicillium oxalicum, a biocontrol agent against fungal diseases and against the potato cyst nematodes (PCNs), Globodera pallida and Globodera rostochiensis. Methods and Results: We tested the effect of P. oxalicum on the nematode cysts under laboratory conditions or in soil microcosms. A reduction in the rate of G. pallida juveniles hatching by P. oxalicum was observed when root diffusates from the 'Monalisa' and the 'D esir ee' potato cultivar were used (98Á6 and 74Á1% reduction, respectively). However, the rate of G. pallida juveniles hatching was not significantly reduced when root diffusates from the 'San Pedro' tomato cultivar were used. Penicillium oxalicum also significantly reduced the ability of the G. rostochiensis juveniles to hatch (30Á9% reduction) when root diffusates of the 'D esir ee' potato cultivars were used. Penicillium oxalicum treatment of the soil significantly reduced the number of G. pallida cysts that were recovered from the soil of each pot that contained the 'D esir ee' potato cultivar. Conclusions: Our results show that P. oxalicum is a potential biocontrol inoculant for protecting potato crops against PCNs. Significance and Impact of the Study: Penicillium oxalicum has potential to be used in order to reduce PCNs.

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“…Polysaccharides from Talaromyces flavus,7) Talaromyces wortmanii, 8) and Eupenicillium crustaceum 9 ) were provided by Dr. J. A. Leal, Centro de Investigationes Biologicas, CSIC, Madrid, Spain.…”

Section: Methodsmentioning

confidence: 99%

“…3) The enzyme did not cleave 13(1 ~ 2) and/or f3(l ~ 3) linkages of galactofuranoside residues of T. wortman ii, 8) galactomann an of S. pombe or the galactopyranosyl linkages present in gum arabic, gum ghati, gum guar, arabi no galactan, araban, and pectic acid. The column (4.6 x 250 mm) was eluted with two solvents as described under Materials and Methods.…”

Section: Galactofuranosidase Action On Polysaccharide Containing Galamentioning

confidence: 99%

Isolation and Characterization of a Novel Endo-β-galactofuranosidase fromBacillussp.

Nahrowi

Fujinaga

Tabuchi

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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Purification of a new galactanase from Penicillium oxalicum catalysing the hydrolysis of β-(1→5)-galactofuran linkages

Cited by 11 publications

References 28 publications

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

Penicillium oxalicum reduces the number of cysts and juveniles of potato cyst nematodes

Isolation and Characterization of a Novel Endo-β-galactofuranosidase fromBacillussp.

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