Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean)

Correia, Maria T.S.; Coelho, Luana Cassandra Breitenbach Barroso

doi:10.1007/bf02786865

Cited by 133 publications

(107 citation statements)

References 37 publications

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“…Hemolytic assay was performed with erythrocytes using, the technique previously described by [25]. The crude plant protein of M. zapota 25 ul were serially diluted to each well containing the same volume of 25 ul TBS buffer, pH 7.4, then 25 ul of 1.5% various vertebrate RBC suspension was added in 96 well 'V' bottom microtitre plate.…”

Section: Determination Of Hemolytic Activitymentioning

confidence: 99%

Entomotoxicity Properties of Eco-Friendly Crude Protein Extract From Manilkara Zapota Seed against Asian Tiger Vector Aedes Aegypti

Ramanibai¹

2017

SOJVS

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Section: Determination Of Hemolytic Activitymentioning

confidence: 99%

Entomotoxicity Properties of Eco-Friendly Crude Protein Extract From Manilkara Zapota Seed against Asian Tiger Vector Aedes Aegypti

Ramanibai¹

2017

SOJVS

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“…The fractions with high activity were bulked and dialyzed with three changes of 0.01 mol L -1 citrate phosphate buffer (pH 6.5) containing 0.15 mol L -1 sodium chloride (1 mL/100 mL/h). Hemagglutinating activity (HA) was determined (Correia and Coelho, 1995); protein concentration was measured according to Lowry et al (1951) and by absorbance at 280 nm. The material was stored at 20°C until used.…”

Section: Purification Of Bmollmentioning

confidence: 99%

Endophytic microorganisms from Bauhinia monandra leaves: Isolation, antimicrobial activities and interaction with galactose-specific lectin BmoLL

Ramos¹,

Silva²,

Correia³

et al. 2016

Afr. J. Microbiol. Res.

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Bauhinia monandra leaves are well known hypoglycemic agent in Brazilian popular medicine from which a galactose specific lectin (BmoLL) has been purified in milligram quantities. In this work, the antimicrobial activity of endophytes isolated from B. monandra leaves and the ability of BmoLL to agglutinate the microorganisms were evaluated. After disinfection, leaves of B. monandra were fragmented and distributed in Petri plates leading to isolation of fungi (37 strains) and both Grampositive (26 strains) and negative bacteria (6 strains). A preliminary antimicrobial assay revealed that 62% of bacterial strains were active, while no antagonist action was detected with fungi. However, only 2 strains were able to excrete the antimicrobial compounds: Pseudomonas aeruginosa UFPEDA598 inhibited Aspergillus niger, Fusarium moniliform, Fusarium oxysporum, Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis and PE(24)C1 inhibited Candida sp. strains, M. luteus and S. aureus. On the other hand, BmoLL did not show any antimicrobial action, but it was able to agglutinate the active strain P. aeruginosa UFPEDA598. The BmoLL-Pseudomonas interaction could promote a defense against the attack of phytopathogenic microorganisms in plants through a P. aeruginosa metabolic production mechanism. The lectins/endophytes interaction could be a new line to unravel defense mechanisms against phytopathogenic organisms.

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“…We determined the MPLEC's hemagglutinating activity (UH) using human ABO and rabbit erythrocytes divided into two groups, processed and not processed by enzymatic treatment of trypsin, bromelain and papain according to Correia and Coelho (1995). UH was defined as the lowest dilution capable of causing hemagglutination.…”

Section: Hemagglutinating Activity and Inhibition Essaysmentioning

confidence: 99%

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

Lacerda¹,

Moreira²,

Nascimento³

et al. 2015

J. Med. Plants Res.

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Lectins are proteins present in all living beings capable of interacting specifically and reversibly to carbohydrates or glycoconjugates form. They stand out in this family of lectins legumes, which are of special scientific interest because they have different biological functions. This study investigated the presence of a lectin in Brazilian seeds of Mucuna, proceeding with their isolation and evaluation of its antinociceptive effects, anti-inflammatory and antihemolytic activity. Saline extracts of Mucuna pruriens were investigated for the presence of hemagglutinating activity by serial dilution and applied to Sephacryl S-200HR columns and DEAE Sephacel HiPrepFF to purify their lectin. After assessing the purity and molecular weight by polyacrylamide agarose gel electrophoresis (PAGE), the hemolytic activity on human erythrocytes was determined, as well as its antinociceptive action by models of writhing induced by acetic acid and formalin, in addition to its anti-inflammatory activity by testing the paw edema induced by carrageenan. The results indicated that Brazilian Mucuna seeds had a lectin that was purified from seeds having molecular weight of 60.0 kDa and only able to agglutinate erythrocytes of rabbit. The lectin showed an antihemolytic effect on human erythrocytes by not causing hemolysis in these cells compared to the negative control. The administration of lectin in rodents intraperitoneally inhibited the writhing by 99% (2.5 mg/kg), induced by acetic acid, as well as in the method where the formalin nociceptive stimulus was reduced by 51% in stage one and 77% in stage two of the test, using 2.5 mg/kg lectin. The anti-inflammatory activity demonstrated a decrease in paw edema induced by carrageenan in 72% better result than indomethacin control where there was a reduction of only 48% of edema. The study supports the presence of a lectin in Brazilian seeds of M. pruriens with antinociceptive, anti-inflammatory and antihemolytic activities.

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Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean)

Cited by 133 publications

References 37 publications

Entomotoxicity Properties of Eco-Friendly Crude Protein Extract From Manilkara Zapota Seed against Asian Tiger Vector Aedes Aegypti

Entomotoxicity Properties of Eco-Friendly Crude Protein Extract From Manilkara Zapota Seed against Asian Tiger Vector Aedes Aegypti

Endophytic microorganisms from Bauhinia monandra leaves: Isolation, antimicrobial activities and interaction with galactose-specific lectin BmoLL

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

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