Purification, modeling and structural insights of calmodulin-binding receptor like cytoplasmic kinase 2 from Oroxylum Indicum

Ali, Nabeel; Amir, Mohd.; Hassan, Md. Imtaiyaz; Ahmad, Faizan; Islam, Asimul

doi:10.1016/j.ijbiomac.2018.11.013

Cited by 4 publications

(2 citation statements)

References 60 publications

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“…In case of HDL level elevation combination therapy indicated a considerable upward trend. This study also supports the previous experiment as simvastatin was treated as a very potential lipid-lowering drug and coincided with prior studies [102][103][104][105][106]. To identify the level enzymes (both SGPT and SGOT) both treatment protocols failed to decrease the level of specific enzyme level to a noteworthy extent and exhibited similarities with past studies [107][108][109][110][111][112][113] but revealed satisfactory decline.…”

Section: Discussionsupporting

confidence: 89%

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Begum

Islam

Begum

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The stem bark of Oroxylum indicum (O. indicum) was aimed at testing for anti-inflammatory, antiulcerative, antihyperglycemic, and antidyslipidemic activities. Liver enzyme concentration (SGPT, SGOT) had also been assessed. After being extracted in organic solvent, 3 distinct doses, 100, 200, and 400 mg/kg b.w. (p.o.), were used. For edema formation 0.1 ml carrageenan at a dose of 1% w/v was injected into paw of left hind. It showed a fall of edemas 37.50%, 48.34%, and 55.83% while used doses were 100, 200, and 400 mg/kg b.w. (p.o.) individually. The EtOH extract of O. indicum (50%) and its fractions PET, CLF, EtOAc, and nBUT were studied against ethanol-induced gastric mucosal damage. Only PET and n-BuOH exhibited the highest percentage of protection and were 96% and 99%, respectively, persuaded by ethanol. In OGTT glibenclamide revealed reduction of glucose level to 7.55 ± 0.22 mmol/L from 10.57 ± 0.32 mmol/L after 30 minutes. Antihyperglycemic activities were assessed for 8- and 12-week duration in diabetic rats. Glibenclamide reduced glucose level from 33.50±0.31 to 7.90±0.19 mmol/L in 12 weeks. In 12 and 8 weeks, combination therapy lowered blood glucose level to a normal extent by 79% and 61% individually. In antidyslipidemic activities after 12-week treatment, it revealed simvastatin; MEOI (400 mg/kg b.w.) and combination of both reduced TC level by 44%, 28%, and 48% consequently followed by TG and LDL. In 8-week treatment, HDL levels were increased by 34%, 13%, and 36%, and in 12 weeks increased by 36%, 8%, and 38% consequently. Liver enzyme concentration after 12 weeks of treatment with glibenclamide, 400 mg/kg b.w. (p.o.) of MEOI and combination of both, exhibited the fact that concentration of SGPT showed downturn by 43.23%, 8.01%, and 54.86% and SGOT by 42.40%, 5.31%, and 44.85%. This study remarked that O. indicum has anti-inflammatory, antiulcer, antidiabetic, and antidyslipidemic potentials but has no ameliorative effect on liver enzyme.

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Section: Discussionsupporting

confidence: 89%

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Begum

Islam

Begum

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…After 3 h of mixing with Ni-NTA resin, the supernatant was washed with washing buffer (50 mM Tris-Cl pH8.0, 300 mM NaCl, 10% Glycerol, 20 mM imidazole). The protein was eluted in the elution buffer, which is the same as washing with the addition of 0.2 M imidazole [ 65 ].…”

Section: Methodsmentioning

confidence: 99%

Mechanistic Insight into the Enzymatic Inhibition of β-Amyrin against Mycobacterial Rv1636: In Silico and In Vitro Approaches

Beg

Sadaf

Shamsi

et al. 2022

Biology

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Mycobacterium tuberculosis has seen tremendous success as it has developed defenses to reside in host alveoli despite various host-related stress circumstances. Rv1636 is a universal stress protein contributing to mycobacterial survival in different host-derived stress conditions. Both ATP and cAMP can be bound with the Rv1636, and their binding actions are independent of one another. β-Amyrin, a triterpenoid compound, is abundant in medicinal plants and has many pharmacological properties and broad therapeutic potential. The current study uses biochemical, biophysical, and computational methods to define the binding of Rv1636 with β-Amyrin. A substantial interaction between β-Amyrin and Rv1636 was discovered by molecular docking studies, which helped decipher the critical residues involved in the binding process. VAL60 is a crucial residue found in the complexes of both Rv1636_β-Amyrin and Rv1636-ATP. Additionally, the Rv1636_β-Amyrin complex was shown to be stable by molecular dynamics simulation studies (MD), with minimal changes observed during the simulation. In silico observations were further complemented by in vitro assays. Successful cloning, expression, and purification of Rv1636 were accomplished using Ni-NTA affinity chromatography. The results of the ATPase activity assay indicated that Rv1636’s ATPase activity was inhibited in the presence of various β-Amyrin concentrations. Additionally, circular dichroism spectroscopy (CD) was used to examine modifications to Rv1636 secondary structure upon binding of β-Amyrin. Finally, isothermal titration calorimetry (ITC) advocated spontaneous binding of β-Amyrin with Rv1636 elucidating the thermodynamics of the Rv1636_β-Amyrin complex. Thus, the study establishes that β-Amyrin binds to Rv1636 with a significant affinity forming a stable complex and inhibiting its ATPase activity. The present study suggests that β-Amyrin might affect the functioning of Rv1636, which makes the bacterium vulnerable to different stress conditions.

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In silico structure prediction of full-length cotton cellulose synthase protein (GhCESA1) and its hierarchical complexes

et al. 2020

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Purification, modeling and structural insights of calmodulin-binding receptor like cytoplasmic kinase 2 from Oroxylum Indicum

Cited by 4 publications

References 60 publications

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Ethnopharmacological Inspections of Organic Extract ofOroxylum indicumin Rat Models: A Promising Natural Gift

Mechanistic Insight into the Enzymatic Inhibition of β-Amyrin against Mycobacterial Rv1636: In Silico and In Vitro Approaches

In silico structure prediction of full-length cotton cellulose synthase protein (GhCESA1) and its hierarchical complexes

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