Purification, Kinetic Properties and Antitumor Activity of L-Glutaminase from Penicillium brevicompactum NRC 829

Elshafei, Ali M.; Hassan, Mohamed M.; Ali, Nadia H.; Abouzeid, Mohamed A.; Mahmoud, Dalia Ali; El-Ghonemy, Dina H.

doi:10.9734/bmrj/2014/5098

Cited by 35 publications

(47 citation statements)

References 23 publications

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“…Maximum glutaminase synthesis by the test organism after the stepwise optimization (46.53 U. ml -1 ) was almost 4.7 folds that of 10 U.ml -1 obtained at the beginning of optimization after one day of fermentation. Results were compared with others in literature and among results of synthesis of glutaminase by mold in literature, only Penicillium brevicompactum showed higher glutaminase synthesis (66.7 U.ml -1 ) (El-Shafei et al, 2014). Otherwise, the tested A. oryzae NRRL 32657 showed higher or equal results.…”

Section: Effect Of Nacl Concentrationmentioning

confidence: 90%

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

Alian¹,

Bazaraa²,

Shimi³

et al. 2015

Afr. J. Microbiol. Res.

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Section: Effect Of Nacl Concentrationmentioning

confidence: 90%

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

Alian¹,

Bazaraa²,

Shimi³

et al. 2015

Afr. J. Microbiol. Res.

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“…Aureus, Pseudo. aeruginosa (Soda et al, 1972;Oshima et al, 1976;Rashmi et al, 2012), E.coli (Pruisner et al, 1976), yeast and filamentous fungi (Elshafei et al, 2014). The production titer value of these enzymes are influenced by microbial strains and fermentation conditions (Iyer and Singhal, 2008).…”

Section: Qualitative Estimation Of L-glutaminase Activitymentioning

confidence: 99%

Determination of L-Glutaminase Activity by Some Bacterial Species

Karim¹,

Thalij²

2016

Int.J.Curr.Microbiol.App.Sci

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“…Aspergillus, Penicillium, and Fusarium, are commonly reported in scientific literature to produce L-asparaginase and L-glutaminase [47][48][49][50]. Mohapatra et al [51] isolated the fungus Mucor sp.…”

Section: Fungal Amidasesmentioning

confidence: 99%

Microbial Amidases and their Industrial Applications: A Review

El-Ghonemy¹

2015

J Med Microb Diagn

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Among the pediatric cancer in developed countries, acute leukemia ¬constitutes the major part with affecting 30-45 per 1,000,000 children each year. The effect of treatment varies with differences in patients clinical, immunologic and genetic characteristics. Therefore, the search for efficient drugs to solve this problem is being continued worldwide. Although several kinds of treatments are available, enzyme therapy is equally effective. Enzymes have been used as drugs; likewise L-asparaginase and L-glutaminase had received much attention in recent years due to their anticarcinogenic potential. These enzymes constitute one of the most biotechnologically and biomedically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales. Various sources are found to be good producers of the enzymes: bacteria, fungi along with some of the plant and animal species. Food and Drug Administration and World Health Organization have approved L-asparaginase for the effective treatment of acute lymphoblastic leukemia and lymphsarcoma. L-asparaginase and L-glutaminase break down L-asparagine or L-glutamine into L-aspartic acid or L-glutamic acid, respectively, and ammonia. L-asparagine depletion results in nutritional deprivation, inhibition of protein synthesis, and subsequent apoptotic cell death in lymphoblasts. On the other hand, the ability of L-asparaginase to selectively hydrolyzes L-asparagine into L-aspartateis a potential way to reduce the amount of free L-asparagine in the starting materials of food production, thus reducing the imminent risk of generating a potential carcinogenic and neurotoxic acrylamide that formed from L-asparagine and reducing sugars in carbohydrate-containing foods (such as snacks and biscuits) when they are heated above 120oC. Therefore, the present review is an attempt to compile information on the sources, antino plastic action and industrial application of microbial amidases enzymes.

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Purification, Kinetic Properties and Antitumor Activity of L-Glutaminase from Penicillium brevicompactum NRC 829

Cited by 35 publications

References 23 publications

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

Determination of L-Glutaminase Activity by Some Bacterial Species

Microbial Amidases and their Industrial Applications: A Review

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