Purification from Human Plasma of a Hexapeptide That Potentiates the Sulfation and Mitogenic Activities of Insulin-like Growth Factors

Dousset, B.; Straczek, J.; Maachi, F.; Nguyen, Duy Linh; Jacob, Christine; Capiaumont, J.; Nabet, P; Belleville, F.

doi:10.1006/bbrc.1998.8834

Cited by 11 publications

(13 citation statements)

References 21 publications

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“…Functionally, both C3f and DRC3f have been demonstrated to enhance vascular permeability and induce smooth muscle contraction as a weak spasmogen (20). The hexapeptide HWESAS, a sequence encompassed within C3f, has recently been found to potentiate the sulfation and mitogenic activities of the IGFs (22). Based on these findings in addition to our own results, we hypothesized that DRC3f could play a role in the pathogenesis of SSc.…”

Section: Discussionsupporting

confidence: 62%

“…In addition, we showed that both the DRC3f-containing serum samples and the filtered DRC3f-containing serum samples (containing only molecules with molecular weights of lower than ϳ3 kd) enhanced the proliferation of HMVECs (Figure 4). These results suggest that DRC3f is one of the low molecular weight growth factors in sera (22,35). However, morphologic abnormalities in the vessels, defective angiogenesis, and apoptosis instead of proliferation of endothelial cells are common features of the microvasculature in SSc.…”

Section: Discussionmentioning

confidence: 83%

“…In a previous study, the hexapeptide HWESAS, which is included in the DRC3f amino acid sequence, was found to promote mitogenic activities of IGF (22). We thus expected that DRC3f might promote cell proliferation via IGF, and thereby play a role in the pathogenesis of SSc.…”

mentioning

confidence: 91%

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Comprehensive investigation of disease‐specific short peptides in sera from patients with systemic sclerosis: Complement C3f‐des‐arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells

Xiang¹,

Matsui²,

Matsuo³

et al. 2007

Arthritis & Rheumatism

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Objective. To identify pathogenic and/or diseasespecific short peptides in sera from patients with systemic sclerosis (SSc).Methods. Serum samples from 40 patients with SSc, 30 patients with systemic lupus erythematosus, 21 patients with rheumatoid arthritis, 30 patients with osteoarthritis, and 26 healthy donors were tested. Short peptides with molecular weights of smaller than ϳ3 kd, purified from the sera by magnetic bead-based hydrophobic interaction chromatography 18, were detected and their amino acid sequences determined using matrix-assisted laser desorption ionization-time-offlight mass spectrometry. Effects of the identified peptides on fibroblasts and microvascular endothelial cells were tested using synthesized peptides and sera containing the peptides.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 83%

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Comprehensive investigation of disease‐specific short peptides in sera from patients with systemic sclerosis: Complement C3f‐des‐arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells

Xiang¹,

Matsui²,

Matsuo³

et al. 2007

Arthritis & Rheumatism

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“…Because our constant routine protocol was conducted under sleep deprivation conditions, it is possible that some of these metabolites might be associated with sleep pressure. For example, we found a more than threefold increase in two fragments of the C3 complement (30). Upregulation of the immune system in general and the C3 complement in particular has been reported in sleep deprivation in humans (31).…”

Section: Discussionsupporting

confidence: 54%

The human circadian metabolome

Dallmann

Viola

Tarokh

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

500

430

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The circadian clock orchestrates many aspects of human physiology, and disruption of this clock has been implicated in various pathologies, ranging from cancer to metabolic syndrome and diabetes. Although there is evidence that metabolism and the circadian clockwork are intimately linked on a transcriptional level, whether these effects are directly under clock control or are mediated by the rest-activity cycle and the timing of food intake is unclear. To answer this question, we conducted an unbiased screen in human subjects of the metabolome of blood plasma and saliva at different times of day. To minimize indirect effects, subjects were kept in a 40-h constant routine of enforced posture, constant dim light, hourly isocaloric meals, and sleep deprivation. Under these conditions, we found that ∼15% of all identified metabolites in plasma and saliva were under circadian control, most notably fatty acids in plasma and amino acids in saliva. Our data suggest that there is a strong direct effect of the endogenous circadian clock on multiple human metabolic pathways that is independent of sleep or feeding. In addition, they identify multiple potential small-molecule biomarkers of human circadian phase and sleep pressure.metabolomics | LC/GC-MS | metabolite profiling | sleep-wake regulation T he circadian clock has been shown to modulate many aspects of behavior and physiology (1). It is thought to be an important regulator of metabolism, and disruption of the clock and sleep is associated with obesity, metabolic syndrome, and type 2 diabetes, as well as other disorders (2-4). In the last decade, ample data on the circadian transcriptome (5, 6) and the even larger circadian proteome (7) have been compiled. These datasets are directly dependent on the genome of a particular species and cannot be compared easily between model systems. However, changes in physiology and metabolism governed by these genes and proteins ultimately affect the abundance of small metabolites that are quite conserved among species and fewer in number (50-fold fewer than transcripts and 500-fold fewer than proteins).The relationship between metabolism and the clock is not unidirectional, and the two processes are intertwined (8). For example, metabolic status feeds back to the clock, so that feeding behavior directly entrains molecular clock function (9). Likewise, obesity is correlated with poor sleep (2), and in mice 80% of circadian transcription in the brain is dependent on the rest-activity cycle (10). Given these feedback mechanisms, it is unclear what proportion of circadian metabolic control is directly clockregulated and what proportion is controlled by circadian restactivity and food intake.In plants, the metabolome approach has been used to characterize the effects of clock disruption on general metabolism (11). The circadian metabolome also has been characterized in CBA/N mice, and ∼20% of the recorded molecules were found to vary in abundance with time of day (12). Similarly, the urine and saliva metabolomes of human subjects diff...

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“…59,60 Complement C3f has biological activity, acting as a mediator for inflammation and potentiating the effects of insulin-like growth factors. 61,62 On the basis of the MS data, the C-terminal arginine is rapidly cleaved from the C3f peptide by carboxypeptidase N, because only the intact peptide is observed to contain the C-terminal Arg residue. In addition, aminopeptidase N degrades the remaining sequence.…”

Section: Resultsmentioning

confidence: 99%

Direct Tandem Mass Spectrometry Reveals Limitations in Protein Profiling Experiments for Plasma Biomarker Discovery

et al. 2005

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The low molecular weight plasma proteome and its biological relevance are not well defined; therefore, experiments were conducted to directly sequence and identify peptides observed in plasma and serum protein profiles. Protein fractionation, matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) profiling, and liquid-chromatography coupled to MALDI tandem mass spectrometry (MS/MS) sequencing were used to analyze the low molecular weight proteome of heparinized plasma. Four fractionation techniques using functionally derivatized 96-well plates were used to extract peptides from plasma. Tandem TOF was successful for identifying peptides up to m/z 5500 with no prior knowledge of the sequence and was also used to verify the sequence assignments for larger ion signals. The peptides (n>250) sequenced in these profiles came from a surprisingly small number of proteins (n approximately 20), which were all common to plasma, including fibrinogen, complement components, antiproteases, and carrier proteins. The cleavage patterns were consistent with those of known plasma proteases, including initial cleavages by thrombin, plasmin and complement proteins, followed by aminopeptidase and carboxypeptidase activity. On the basis of these data, we discuss limitations in biomarker discovery in the low molecular weight plasma or serum proteome using crude fractionation coupled to MALDI-MS profiling.

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Purification from Human Plasma of a Hexapeptide That Potentiates the Sulfation and Mitogenic Activities of Insulin-like Growth Factors

Cited by 11 publications

References 21 publications

Comprehensive investigation of disease‐specific short peptides in sera from patients with systemic sclerosis: Complement C3f‐des‐arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells

Comprehensive investigation of disease‐specific short peptides in sera from patients with systemic sclerosis: Complement C3f‐des‐arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells

The human circadian metabolome

Direct Tandem Mass Spectrometry Reveals Limitations in Protein Profiling Experiments for Plasma Biomarker Discovery

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