Purification, crystallization and preliminary X-ray structure analysis of the laccase from<i>Ganoderma lucidum</i>

Lyashenko, A. V.; Belova, O. V.; Габдулхаков, А.Г.; Lashkov, A.A.; Lisov, Alexandr V.; Leontievsky, A. A.; Mikhailov, A.M.

doi:10.1107/s1744309111021129

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…Laccases are copper-containing polyphenol oxidases and are homologous with ascorbate oxidase and with mammalian plasma protein ceruloplasmin, which belong to the blue multi-copper oxidase family and have several similarities in structure and functionality 1 . Laccases play vital roles in the natural carbon cycle and facilitate direct reduction of molecular oxygen to water 2 . Even in the absence of H 2 O 2 and of other secondary metabolites, the substrate may be directly oxidized by laccases as long as dissolved oxygen exists 3 .…”

Section: Introductionmentioning

confidence: 99%

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Sun

Wang

et al. 2017

Sci Rep

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Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the laccase output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of laccase synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of laccase. The results indicate that the accumulation of superoxide anion radical (O2 −) and hydrogen peroxide (H2O2) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and chalcone isomerase. This phenomenon is probably the result of the activated phenylpropanoids–flavonoid pathway. Results confirmed that the mixture culture is advantageous for laccase production and revealed that NO, O2 −, and H2O2 are necessary signal molecules to induce laccase synthesis.

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Section: Introductionmentioning

confidence: 99%

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Sun

Wang

et al. 2017

Sci Rep

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“…Except for these, LZ-8, a protein isolated from G. lucidum mycelia, has been shown to have immunomodulating activity [12] , [24] , and its structural, biochemical and immunological properties have been studied in much detail [25] – [28] . Moreover, numerous laccase isozymes which have ligninolytic capabilities have also been identified from G. lucidum [6] , [7] , [29] .…”

Section: Introductionmentioning

confidence: 99%

Deep Insight into the Ganoderma lucidum by Comprehensive Analysis of Its Transcriptome

Wang

Huang

et al. 2012

PLoS ONE

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Background Ganoderma lucidum is a basidiomycete white rot fungus and is of medicinal importance in China, Japan and other countries in the Asiatic region. To date, much research has been performed in identifying the medicinal ingredients in Ganoderma lucidum. Despite its important therapeutic effects in disease, little is known about Ganoderma lucidum at the genomic level. In order to gain a molecular understanding of this fungus, we utilized Illumina high-throughput technology to sequence and analyze the transcriptome of Ganoderma lucidum.Methodology/Principal FindingsWe obtained 6,439,690 and 6,416,670 high-quality reads from the mycelium and fruiting body of Ganoderma lucidum, and these were assembled to form 18,892 and 27,408 unigenes, respectively. A similarity search was performed against the NCBI non-redundant nucleotide database and a customized database composed of five fungal genomes. 11,098 and 8, 775 unigenes were matched to the NCBI non-redundant nucleotide database and our customized database, respectively. All unigenes were subjected to annotation by Gene Ontology, Eukaryotic Orthologous Group terms and Kyoto Encyclopedia of Genes and Genomes. Differentially expressed genes from the Ganoderma lucidum mycelium and fruiting body stage were analyzed, resulting in the identification of 13 unigenes which are involved in the terpenoid backbone biosynthesis pathway. Quantitative real-time PCR was used to confirm the expression levels of these unigenes. Ganoderma lucidum was also studied for wood degrading activity and a total of 22 putative FOLymes (fungal oxidative lignin enzymes) and 120 CAZymes (carbohydrate-active enzymes) were predicted from our Ganoderma lucidum transcriptome.ConclusionsOur study provides comprehensive gene expression information on Ganoderma lucidum at the transcriptional level, which will form the foundation for functional genomics studies in this fungus. The use of Illumina sequencing technology has made de novo transcriptome assembly and gene expression analysis possible in species that lack full genome information.

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“…The molecular structures of laccases have been reported by several groups. − Briozzo et al and Piotek et al independently first succeeded in determining the structure of a laccase from Trametes versicolor . , A visible representation of the structure of this enzyme is shown in Figure . This laccase can be divided into three domains and has four copper ions, and the T1 copper ion locates in domain 3, and the trinuclear copper cluster (T2, T3a, and T3b) is embedded between domains 1 and 3 (Figure ).…”

Section: Introductionmentioning

confidence: 99%

“…Since then, numerous papers have been published on this unique enzyme; 12 952 references are currently listed for the topic of “laccase*” in the database of the Web of Science Core Collections (September 17, 2020). Laccases are now used in many fields of biotechnology such as biopulping, textile dye bleaching, bioremediation, biological fuel cells, and biosensors. − Laccases belong to the blue-copper family of oxidases that mediate the oxidative degradation or polymerization of polyphenol formation in plants by inducing the radical polymerization of polyphenol derivatives; thus, laccases are widely found in plants, fungi, bacteria, and insects. − …”

Section: Introductionmentioning

confidence: 99%

Laccase-Catalyzed Reactions in Ionic Liquids for Green Sustainable Chemistry

Itoh

Takagi

2020

ACS Sustainable Chem. Eng.

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Biocatalysis is an important and enabling tool in organic synthesis, degradation of chemical compounds, and biosensors. Laccases are copper ions containing oxidase and catalyze the conversion of polyphenol or amine derivatives using molecular oxygen. Recently, significant interests have gained attention for the development of a biochemical degradation method of lignocellulosic materials from the standpoint of green sustainable chemistry. Since these compounds are highly oxidized compounds, it is difficult to convert them stereo-or chemo-selectively to high-value small molecules. Chemical oxidations generally require hazardous reagents and harsh reaction conditions; hence, the resulting produced compounds are generally low-value molecules or complicated mixtures. On the contrary, laccases allow hazardous-reagent-free highly selective oxidative chemical conversions. The reactivity of the enzymes is influenced by a set of reaction conditions, in particular, the solvent system. Ionic liquids (ILs) have now been acknowledged as useful reaction media for biotransformations. Laccase-mediated reactions in ILs have been reviewed and focused on four topics: the first is "laccase-catalyzed reactions in ILs". The second is "biosensor system using laccase-mediated reaction in ILs". The third is "improved stability of laccase by immobilization or IL type additives", and the last chapter is "improved activity of laccase in ILs by protein engineering". Our opinion toward future perspectives of laccase-mediated reactions has also been discussed in "conclusions and future perspectives".

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Purification, crystallization and preliminary X-ray structure analysis of the laccase fromGanoderma lucidum

Cited by 6 publications

References 39 publications

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production

Deep Insight into the Ganoderma lucidum by Comprehensive Analysis of Its Transcriptome

Laccase-Catalyzed Reactions in Ionic Liquids for Green Sustainable Chemistry

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