Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica

Abstract

An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α-l-Fucp and (1→4)-α-l-Fucp residues with a small degree of branching at C-3 of (1→4)-α-l-Fucp residues. Sulfate groups were at C-4 of (1→3)-α-l-Fucp, C-2 of (1→4)-α-l-Fucp and minor C-6 of (1→4)-β-d-Galp. Small amounts of xylose and galactose exist in the forms of β-d-Xylp-(1→ and β-d-Gal-(1→. The immunomodulatory activity of DAP4 was measured on RAW 264.7 cells, the results proved that DAP4 exhibited excellent immunomodulatory activities, such as promoted the proliferation of spleen lymphocytes, increased NO production, as well as enhanced phagocytic of macrophages. Besides, DAP4 could also produce better enhancement on the vitality of NK cells. For the high immunomodulatory activity, DAP4 might be a potential source of immunomodulatory fucoidan with a novel structure.

Hypoglycemic activity in vitro and vivo of a water-soluble polysaccharide from Astragalus membranaceus

“…In addition, the characteristic absorption at 1413 cm −1 was derived from the variable angle vibration of C–H. 38 The absorptions at around 1740 cm −1 and 1631 cm −1 corresponded to the vibration of esterified and free carboxyl groups, confirming the presence of uronic acid. 39 The absorption at 1236 cm −1 was attributed to the C–O stretching vibration.…”

Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans

“…The band at 1633 cm −1 was attributed to the acyl group [ 35 ]. The peak of 1594 cm −1 was due to the bending vibration of O–H [ 36 ]. The band at 1215–1255 cm −1 was attributed to the existence of sulfate group, which was a characteristic component of fucoidan.…”

Analysis of Muscle Lipidome in Juvenile Rainbow Trout Fed Rapeseed Oil and Cochayuyo Meal

“…This could be due to polysaccharides in seaweeds, as cochayuyo can stimulate lipid metabolism. The body catabolizes fatty acids as the primary energy expenditure, explaining the increase in phospholipid species detected in this study [ 49 , 50 ].…”