Purification, characterization of alkaline protease enzyme from native isolate Aspergillus niger and its compatibility with commercial detergents

Devi, Monisha

doi:10.17485/ijst/2008/v1i7.8

Cited by 36 publications

(37 citation statements)

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“…Therefore, we can say that alkaline protease produced from A. terreus is being promising additive components for local laundry detergents. Stability and compatibility of the alkaline protease of Aspergillus species with detergents has been previously recorded 20,21,25,33 .…”

Section: Discussionmentioning

confidence: 99%

“…The specificity of the enzyme against a number of protein substrates recorded 10 mg/mL of casein as the best substrate for maximum protease activity. The same substrate was recorded for A. tamari protease 41 and A. niger 20 . Michaelis constant Km value of the purified enzyme was calculated to be 6.66 mg/mL.…”

Section: Discussionmentioning

confidence: 99%

“…The maximal productivity was reported at pH 10 and this finding was in agree with one that recorded the same initial pH of protease from Aspergillus sp. 20,24 .…”

Section: Discussionmentioning

confidence: 99%

“…2.9 Optimization of alkaline protease production parameters The SSF was also optimized through various parameters including moisture content of substrate 58, 71.5, 78.3, 82 and 85 , incubation period 2, 3, 4, 5, 6 and 7 days , incubation temperature 30,35,40,45,50,55 and 60 , size of inoculum 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mL of spores suspension and initial pH of basal medium 8.0, 8.5, 9.0, 9.5, 10.0, 10.5 and 11.0 . The effect of supplementation of the basal medium with different by-products whey, cane molasses, beet molasses and corn steep liquor as additives 5 , v/v and effect of different concentrations of whey 5,10,15,20,25, and 30 were also ascertained.…”

Section: Protein Determinationmentioning

confidence: 99%

“…Detergents were diluted to a concentration of 7.0 mg/mL to mimic washing condition. The diluted detergent was heated at 95 for 10 min to inactivate the endogenous enzymes found in it 20,21 . A reaction mixture comprising 0.3 mL of crude enzyme 34.87 U/mg protein and 5.7 mL detergent was preincubated at 28, 60 and 90 .…”

Section: Determination Of Kinetic Parametersmentioning

confidence: 99%

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Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

2020

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Introduction Protease enzymes EC 3.4.21-24 are fit for hydrolyzing the bonds between amino acids of protein molecule and they are the major industrial enzymes, which represented 60 of the complete synthetically produced enzymes 1. Alkaline proteases of microbial origin have impressive industrial intensity because of their biochemical decent variety and enormous applications in detergents, pharmaceutical, nourishment, dairy and leather industries 2. Fungi are the most microbes producing proteases with many advantages, since they are normally GRAS generally regarded as safe strains and their enzymes are secreted outside the cell which easily recover the enzyme 3, 4. Despite the fact that fungi are significant wellsprings of alkaline proteases 5, 6 , studies on their protease production are confined for the reason that the fungi prefer acidic and neutral pH values. Thus, it is of prime interest to search for

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The maximal productivity was reported at pH 10 and this finding was in agree with one that recorded the same initial pH of protease from Aspergillus sp. 20,24 .…”

Section: Discussionmentioning

confidence: 99%

Section: Protein Determinationmentioning

confidence: 99%

Section: Determination Of Kinetic Parametersmentioning

confidence: 99%

See 3 more Smart Citations

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

2020

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Fungal biomolecules for the food industry

Nguyen

Bujna

Styevkó

et al. 2015

Fungal Biomolecules

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Production of the prolyl endoprotease (PEP) from Aspergillus sp. FSDE 16 by solid‐state fermentation (SSF) and use for producing a gluten‐free beer

Almeida,

Santos,

Santos

2024

Biotech and App Biochem

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Beer is a beverage that contains gluten and cannot be consumed by people with celiac disease. In this context, the enzyme prolyl endoprotease (PEP) can be used to reduce the gluten content in beer. The present study aimed to produce the PEP from Aspergillus sp. FSDE 16 using solid‐state fermentation with 5 conditions and comparing with a similar commercial enzyme produced from Aspergillus niger in the production of a gluten‐free beer. The results of the performed cultures showed that during the culture, the most increased protease activity (54.46 U/mL) occurred on the 4th day. In contrast, for PEP, the highest activity (0.0356 U/mL) was obtained on the 3rd day of culture in condition. Regarding beer production, cell growth, pH, and total soluble solids showed similar behavior over the 7 days for beers produced without enzyme addition or with the addition of commercial enzyme and with the addition of the enzyme extract produced. The addition of the enzyme and the enzyme extract did not promote changes, and all the beers produced showed similar and satisfactory results, with acid pH between 4 and 5, total soluble solids ranging from 4.80 to 5.05, alcohol content ranging from 2.83% to 3.08%, and all beers having a dark character with deep amber and light copper color. Gluten removal was effectively using the commercial enzyme and the enzyme produced according to condition (v) reaching gluten concentrations equal to 17 ± 5.31 and 21.19 ± 11.28 ppm, respectively. In this way, the production of the enzyme by SSF and its application in the removal of gluten in beer was efficient.

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Purification, characterization of alkaline protease enzyme from native isolate Aspergillus niger and its compatibility with commercial detergents

Cited by 36 publications

References 0 publications

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

Fungal biomolecules for the food industry

Production of the prolyl endoprotease (PEP) from Aspergillus sp. FSDE 16 by solid‐state fermentation (SSF) and use for producing a gluten‐free beer

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