Purification, characterization and optimization conditions of protease produced by Aspergillus brasiliensis strain BCW2

Vermiculite as a new carrier for extracellular protease production by Aspergillus spp. under solid-state fermentation

“…Most studies on the formation of extracellular proteases by Aspergillus are carried out during their cultivation on agroindustrial wastes [ 19 , 21 , 22 ], which limits the possibility of scaling these processes and their industrial application. In cases with inert carriers, a high yield of fungal enzymes was previously noted during growth on polyurethane foam and perlite in comparison with the conditions of submerged cultivation [ [23] , [24] , [25] ].…”

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

“…Such methodologies are used either individually or in combinations, accompanied by chromatographic techniques for further purification. Chromatofocusing, fast protein liquid chromatography, high performance liquid chromatography, affinity column chromatography, gel filtration chromatography, ion exchange chromatography and hydrophobic interaction chromatography are commonly employed techniques for fibrinolytic enzyme purification [2,5,[100][101][102][103][104][105][106][107][108]110,[162][163][164][165][166][167][168]. Some recent purification studies of microbial fibrinolytic enzymes employed by researchers are discussed below.…”

“…Fungal enzymes, such as Aspergillus ustus 1, Arthrobotrys longa 1 and some others are able to hydrolyse fibrin at pH 6.0 [30,176]. Optimal temperature widely ranged from 20-70 • C [20,31,42,100,101,177,180], mostly approx. 50 • C for bacterial proteases [5,181] and 37-45 • C for some fungal species [20,30,62,101,104,107].…”

“…Optimal temperature widely ranged from 20-70 • C [20,31,42,100,101,177,180], mostly approx. 50 • C for bacterial proteases [5,181] and 37-45 • C for some fungal species [20,30,62,101,104,107]. The molecular weight of fibrinolytic enzymes of different origins broadly varied from 14 kDa to 97 kDa [56,140,172], mostly within 27 kDa and 44 kDa [5,9,182,183].…”

Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme

“…Peptidases are enzymes that have been produced by microorganisms in different types of culture media based on agro-industrial waste, such as wheat bran and soybean fl our (Xiao et al 2005, Meena et al 2013, Semenova et al 2020. The use of proteases for therapeutic applications has been one of the goals of the pharmaceutical industry in recent years, since the catalytic activity of these enzymes permits the use of lower doses for treatments, with a target potential and greater efficiency, and reduce side effects of existing drugs, while maintaining the desired therapeutic benefits and reducing costs becoming interesting for industrial pharmaceutical industry, it is estimated that about 5 to 10% of all pharmaceutical targets for drug development are proteases (Al-Dhabi et al 2020, Chimbekujwo et al 2020. Over the last decade, the search for other proteases from various sources has been under way, being microorganisms are excellent sources of these enzymes because they have wide biochemical diversity, can be easily cultivated and maintained at low cost, and can be genetically manipulated to improve or modify the final product (Zheng et al 2020, Barzkar 2020.…”