Purification, characterization and mechanism of action of enterocin HDX-2, a novel class IIa bacteriocin produced by Enterococcus faecium HDX-2

Du, Renpeng; Ping, Wenxiang; Ge, Jingping

doi:10.1016/j.lwt.2021.112451

Cited by 20 publications

(6 citation statements)

References 37 publications

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“…In another study, lacticin was shown to disrupt the cell membrane permeability of L. monocytogenes resulting in rapid efflux of potassium ions that peaked at 2.5 min (McAuliffe et al 1998 ). However, in a recent study enterocin HDX-2 at 1X MIC (5 µg/ml) was shown to cause maximum efflux of potassium ions from L. monocytogenes cells at 160 min (Du et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Biopreservative efficacy of Enterococcus faecium-immobilised film and its enterocin against Salmonella enterica

et al. 2023

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The growing awareness about the adverse health effects of artificial synthetic preservatives has led to a rapid increase in the demand for safe food preservation techniques and bio preservatives. Thus, in this study, the biopreservatives efficacy of enterocin-producing Enterococcus faecium Smr18 and its enterocin, ESmr18 was evaluated against Salmonella enterica contamination in chicken samples. E. faecium Smr18 is susceptible to the antibiotics penicillin-G, ampicillin, vancomycin, and erythromycin, thereby indicating that it is a nonpathogenic strain. Further, the enterocin ESmr18 was purified and characterised as a 3.8 kDa peptide. It possessed broad spectrum antibacterial activity against both Gram-positive and Gram-negative pathogens including S. enterica serotypes Typhi and Typhimurium. Purified ESmr18 disrupted the cell membrane permeability of the target cell thereby causing rapid efflux of potassium ions from L. monocytogenes and S. enterica. Chicken samples inoculated with S. enterica and packaged in alginate films containing immobilised viable E. faecium resulted in 3 log10 colony forming units (CFU) reduction in the counts of S. enterica after 34 days of storage at 7–8 °C. The crude preparation of ESmr18 also significantly (p < 0.05) reduced the CFU counts of salmonella-inoculated chicken meat model. Purified ESmr18 at the concentration upto 4.98 µg/ml had no cytolytic effect against human red blood cells. Crude preparation of ESmr18 when orally administered in fish did not cause any significant (p < 0.05) change in the biochemical parameters of sera samples. Nonsignificant changes in the parameters of comet and micronucleus assays were observed between the treated and untreated groups of fishes that further indicated the safety profile of the enterocin ESmr18.

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Section: Discussionmentioning

confidence: 99%

Biopreservative efficacy of Enterococcus faecium-immobilised film and its enterocin against Salmonella enterica

et al. 2023

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“…Установлено, что даже один курс пробиотикотерапии оказывает положительное влияние на биологические свойства энтерококков. О роли этих микроорганизмов в кишечном микробиоме идут споры, так как их функции и свойства при эубиозе [4,23,18] и при микроэкологических нарушениях отличаются [6,24]. Они могут быть продуцентами бактериоцинов, органических кислот -молочной, уксуснойс выраженным антибактериальным действием [18,23].…”

Section: Discussionunclassified

“…О роли этих микроорганизмов в кишечном микробиоме идут споры, так как их функции и свойства при эубиозе [4,23,18] и при микроэкологических нарушениях отличаются [6,24]. Они могут быть продуцентами бактериоцинов, органических кислот -молочной, уксуснойс выраженным антибактериальным действием [18,23]. Но при определенных условиях энтерококки способны проявлять вирулентностьсинтезировать гемолизины, липазу, желатиназу, гиалуронидазу [10].…”

Section: Discussionunclassified

Microbiological evaluation of probiotic therapy in patients with pulmonary tuberculosis

Otdushkina,

Zakharova,

Kholodov

et al. 2023

Russian Journal of Infection and Immunity

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Patients with pulmonary tuberculosis and multiple pathogen resistance (MDR) develop persistent disorders of the intestinal microbiome during prolonged multicomponent chemotherapy requiring correction. However, there is limited data on the use of bacterial drugs in patients with tuberculosis followed by assessing their effectiveness. The aim of the study was to evaluate changes in the intestinal microbiome after a course of probiotics along with anti-tuberculosis chemotherapy in patients with MDR tuberculosis. Materials and methods. The design a prospective small-cohort study (n = 30). Patients with pulmonary tuberculosis received anti-tuberculosis drugs according to the IV or V regimen, the median of the doses taken was 34.5 (30; 57.5); gastrointestinal syndrome was recorded in all study subjects. Probiotic therapy was applied by using a preparation containing Bifidobacterium bifidum and B. animalis and Lactobacillus casei, L. plantarum, L. delbrueckii subsp.bulgaricus, L. acidophilus. The course of therapy comprised 21 days,1 capsule twice a day. Before and 7 days after probiotics therapy, studies on composition of the intestinal microbiota were carried out, the frequency of virulence factors Enterococcus spp., Staphylococcus spp., Candida spp. was examined; fatty acid composition and activity of enterococcal organic acid production were studied. Results. After a course of probiotics, a significant increase in lactobacillus titers was recorded from 5.2 (4.0; 6.0) to 6.1 (6.0; 8.0) lg CFU/g (p = 0.05). The frequency of mucosal colonization by Candida fungi and lactose-negative Escherichia decreased by 2-fold (p = 0.001) and 3-fold (p = 0.05), respectively. The frequency of detected virulent strains significantly decreased: hemolysin-producing staphylococci by 9 times (p = 0.009), enterococci with gelatinase activity by 6 times. E. faecalis membrane oleic acid level significantly increased (C9-C18:1) (p = 0.03). In E. faecium, cis-7-palmitoleic acid (C7-C16:1) and oleic (C9-C18:1) fatty acid level increased by 2-fold (p = 0.05), and for linoleic acid (C18:2) by 4 time (p = 0.04) accompanied by elevated acid formation by 1.5 times. Conclusion. A single course of probiotic therapy in patients with pulmonary tuberculosis leads to qualitative microbiome changes, which are characterized by decreased levels of conditionally pathogenic microorganisms with virulent properties and altered composition of the enterococcal cell membrane accompanied by their increased biochemical activity.

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“… [50] where they found bacteriocin LD4 from L. plantarum LD4 strain retaining activity after treatment with different organic solvents. Likewise, enterocin HDX-2 was treated with different organic solvents, surfactants, and detergents and was observed to be stable [26] .…”

Section: Discussionmentioning

confidence: 99%

“…The sample depicting a single peak protein from the CM-ion chromatography was subjected to Tris-Tricine SDS-PAGE analysis to determine its molecular weight [ 26 , 27 ]. The purified protein along with molecular weight markers (Dual Xtra standards, BIORAD, USA) was initially electrophoresed at 40 V for 30 min, thereafter at 100 V for 120 min.…”

Section: Methodsmentioning

confidence: 99%

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

Otunba¹,

Osuntoki

Okunowo

et al. 2022

Biotechnology Reports

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Purification, characterization and mechanism of action of enterocin HDX-2, a novel class IIa bacteriocin produced by Enterococcus faecium HDX-2

Cited by 20 publications

References 37 publications

Biopreservative efficacy of Enterococcus faecium-immobilised film and its enterocin against Salmonella enterica

Biopreservative efficacy of Enterococcus faecium-immobilised film and its enterocin against Salmonella enterica

Microbiological evaluation of probiotic therapy in patients with pulmonary tuberculosis

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

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