Purification, characterization, and gene cloning of two laccase isoenzymes (Lac1 and Lac2) from Trametes hirsuta MX2 and their potential in dye decolorization

Huang, Qianqian; Wang, Chenzhi; Zhu, Ling; Zhang, Dayu; Pan, Cong

doi:10.1007/s11033-019-05154-2

Cited by 16 publications

(17 citation statements)

References 45 publications

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“…Three inducible laccases of LacC, LacD, and LacF have been also expressed in P. canescens , and the activities of recombinant laccases were more than 100 U/mL in the culture broth [ 26 ]. In this study, Lac1, as an inducible laccase of group B from T. hirsuta MX2 [ 8 ], was firstly expressed in P. pastoris . A yeast recombinant with high laccase activity was screened, and its activity was five times higher than that of the original strain [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…The removal of dyes by adsorption, coagulation, ozonation, and chemical degradation is often high-cost and can generate hazardous by-products [ 6 ]. Biodegradation of synthetic dyes by enzymes has attracted increasing attention in recent years because its advantage of high efficiency and eco-friendly procedures in the treatment of dye effluent [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Trametes hirsuta is a basidiomycete fungus that can secrete a variety of laccase isoenzymes [ 8 , 16 , 17 ]. Laccases obtained from different strains of T. hirsuta showed dissimilar decolorization ability to various types of dyes.…”

Section: Introductionmentioning

confidence: 99%

“…One was induced from Trametesvillosa by 2,5-xylidine, and its optimal pH was between 5 and 5.5 with syringaldazine as a substrate [ 27 ]. Another one was produced from T. hirsuta by growing in wood-based medium, and had an optimal pH of 2.5 to ABTS [ 8 ]. The limited data do not allow us to make definite inferences about group B laccase properties.…”

Section: Introductionmentioning

confidence: 99%

“…hirsuta MX2, and was identified as an inducible group B laccase by comparing its encoding gene with other fungal laccase gene sequences. Lac1 efficiently decolorizes synthetic dyes in the present of ABTS, but its yield from the original strain is relatively low to meet the industrial demand [ 8 ]. In this study, in order to increase the yield of Lac1, it was heterologously expressed in P. pastoris .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Recombinant Laccase B from Trametes hirsuta MX2 and Its Application for Decolorization of Dyes

Jia

Huang

Zhu³

et al. 2022

Molecules

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Trametes hirsuta is able to secrete laccase isoenzymes including constitutive and inducible forms, and has potential application for bioremediation of environmental pollutants. Here, an inducible group B laccase from T. hirsuta MX2 was heterologously expressed in Pichia pastoris, and its yield reached 2.59 U/mL after 5 days of methanol inducing culture. The optimal pH and temperature of recombinant laccase (rLac1) to 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were 2.5 and 60 °C, respectively. Metal ions showed different effect on rLac1 which Mg2+, Cu2+, and K+ increased enzyme activity as their concentration increased, whereas Zn2+, Na+, and Fe2+ inhibited enzyme activity as their concentration increased. rLac1 showed good tolerance to organic solvents, and more than 42% of its initial activity remained in 10% organic solvents. Additionally, rLac1 exhibited a more efficient decolorization ability for remazol brilliant blue R (RBBR) than for acid red 1 (AR1), crystal violet (CV), and neutral red (NR). Molecular docking results showed RBBR has a stronger binding affinity with laccase than other dyes by interacting with substrate binding cavity of enzyme. The results indicated rLac1 may be a potential candidate for dye removal from textile wastewater.

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Section: Discussionmentioning

confidence: 99%