Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala

Sharma, Mamta; Hegde, Prajna; Hiremath, Kavita Y.; H, Vishwanath Reddy; Kamalanathan, A.S.; Swamy, Bale M.

doi:10.1007/s10719-018-9843-6

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Owing to their biological and biomedical significance, plant lectins have been subjected to extensive glycan microarray-based investigations to determine their glycan-binding specificities. 108–111 We do not describe here the detailed binding patterns of plant lectins obtained from glycan microarray experiments because the binding specificity data of many plant lectins can be found at the CFG website.…”

Section: Applicationsmentioning

confidence: 99%

Glycan microarrays from construction to applications

2022

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Section: Applicationsmentioning

confidence: 99%

Glycan microarrays from construction to applications

2022

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“…For example, leaves of A. cissampeloides contain tannins, saponins, phlobatannins, terpenoids, steroids, and alkaloids [19], while leaves of A. lobata contain catechic tannins, sterols/triterpenes, and alkaloids [20]. Other than leaves, roots of A. hondala contain N-acetyl galactosamine [21], while caudices of 10 Adenia species (A. ellenbeckii, A. fruticosa, A. glauca, A. goetzii, A. keramanthus, A. lanceolata, A. racemosa, A. spinosa, A. stenodactyla, and A. venenata) contain galactose-recognizing lectins [22]. Some of these lectins could strongly inhibit protein synthesis in cancer cells, leading to cell death and potential control of cancer [22].…”

Section: Adenia Viridiflora Craib Is a Wild Indigenous Climbing Plant Found In Northeasternmentioning

confidence: 99%

In Vitro Phytotherapeutic Properties of Aqueous Extracted Adenia viridiflora Craib. towards Civilization Diseases

et al. 2021

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Adenia viridiflora Craib. is an indigenous edible plant that became an endangered species due to limited consumption of the local population with unknown reproduction and growth conditions. The plant is used as a traditional herb; however, its health applications lack scientific-based evidence. A. viridiflora Craib. plant parts (old leaves and young shoots) from four areas as Kamphaeng Phet (KP), Muang Nakhon Ratchasima (MN), Pakchong Nakhon Ratchasima (PN), and Uthai Thani (UT) origins were investigated for phenolic compositions and in vitro health properties through the inhibition of key enzymes relevant to obesity (lipase), diabetes (α-glucosidase and dipeptidyl peptidase-IV), Alzheimer’s disease (cholinesterases and β-secretase), and hypertension (angiotensin-converting enzyme). Phenolics including p-coumaric acid, sinapic acid, naringenin, and apigenin were detected in old leaves and young shoots in all plant origins. Old leaves exhibited higher total phenolic contents (TPCs) and total flavonoid contents (TFCs), leading to higher enzyme inhibitory activities than young shoots. Besides, PN and MN with higher TPCs and TFCs tended to exhibit greater enzyme inhibitory activities than others. These results will be useful to promote this plant as a healthy food with valuable medicinal capacities to support its consumption and agricultural stimulation, leading to sustainable conservation of this endangered species.

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“…The type 2 RIPs so far known belong to a few plant genera [ 18 , 19 ]; among them, the most known cytotoxic RIPs are abrin, ricin, and several RIPs purified from Adenia plants. Modeccin and volkensin, from Adenia (Modecca) digitata (Harv.)…”

Section: Introductionmentioning

confidence: 99%

Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties

Bortolotti,

Biscotti,

Zanello

et al. 2023

Toxins

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Ribosome-inactivating proteins (RIPs) are plant toxins that were identified for their ability to irreversibly damage ribosomes, thereby causing arrest of protein synthesis and induction of cell death. The RIPs purified from Adenia plants are the most potent ones. Here, we describe a novel toxic lectin from Adenia heterophylla caudex, which has been named heterophyllin. Heterophyllin shows the enzymatic and lectin properties of type 2 RIPs. Interestingly, in immunoreactivity experiments, heterophyllin poorly cross-reacts with sera against all other tested RIPs. The cytotoxic effects and death pathways triggered by heterophyllin were investigated in three human-derived cell lines: NB100, T24, and MCF7, and compared to ricin, the most known and studied type 2 RIP. Heterophyllin was able to completely abolish cell viability at nM concentration. A strong induction of apoptosis, but not necrosis, and the involvement of oxidative stress and necroptosis were observed in all the tested cell lines. Therefore, the enzymatic, immunological, and biological activities of heterophyllin make it an interesting molecule, worthy of further in-depth analysis to verify its possible pharmacological application.

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Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala

Cited by 4 publications

References 37 publications

Glycan microarrays from construction to applications

Glycan microarrays from construction to applications

In Vitro Phytotherapeutic Properties of Aqueous Extracted Adenia viridiflora Craib. towards Civilization Diseases

Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties

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