Purification, characterization and evaluation of extracellular peroxidase from two species for aqueous phenol treatment

Oxidative coupling of various aromatic phenols and anilines in water using a laccase from Trametes villosa and insights into the ‘PEG effect’

“…However, the exact mechanism by which PEG protects the enzyme, or how to reliably predict what substrate-enzyme-additive combination will work, are still not clear. The fact that crude enzymes have a smaller PEG effect than purified ones (clearly demonstrated by the Coprinus UAMH results (57) in Table 1(A)) supports Nakamoto and Machida's sacrificial polymer theory. A working hypothesis for the 'PEG effect' is emerging, based on the following tenets: (a) PEG adsorbs to the polyphenolic precipitate, making the latter's surface unattractive to the enzyme; (b) different enzymes vary in their relative affinities for the polymeric precipitate, hence in their sensitivity to additives like PEG; (c) the relative hydrophobicities of the polyphenolic precipitates are modulated by the nature of the participating monomers.…”

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

“…The findings revealed that the maximum activity recorded for LiP-SN was obtained with an initial H 2 O 2 concentration of about 0.33 mM. This result is consistent with previous findings for other peroxidases [28]. The H 2 O 2 concentrations were also noted to exert inhibitory effects on enzyme activity, which were comparable to other peroxidases [29,30].…”

Biodepollution of wastewater containing phenolic compounds from leather industry by plant peroxidases

“…Most of the phenoxy radicals interact with PEG due to their greater affinity with PEG than the enzyme, thus preventing the adsorption of the reaction product on the active sites of enzyme molecules (Tonegawa et al 2003;Gonzalez et al 2008). Thus as previously found (Ikehata et al 2003(Ikehata et al , 2005, the addition of PEG in the reaction mixture increased both the rate and the yield of degradation. The enzyme reaction in this method can be considered as a modified type of ping-pong kinetics, referred as peroxidase ping-pong by Dunford (1991).…”