Purification, characterization and biotechnological application of an alkaline β-keratinase produced by Bacillus subtilis RM-01 in solid-state fermentation using chicken-feather as substrate

Isolation of a feather-degrading Bacillus subtilis strain from the alimentary tract of grebes

“…As a result, the active range of pH for keratinase was between pH 6.0 and pH 9.0 whereas the optimal reaction pH of keratinase was determined to be 6.5 (Figure 8), differing from the reported keratinases from B. subtilis strains (Suh and Lee, 2001;Cai et al, 2008b;Balaji et al, 2008;Rai et al, 2009;Jeong et al, 2010;Mazotto et al, 2011). High level of keratinase activity was observed in a range of 30-60°C.…”

Microbial keratinases: characteristics, biotechnological applications and potential.

“…The application of keratinases in the detergent industry has been most promising as many of these alkaline proteases are thermally stable at wash temperature and tolerant of surfactants P a g e | 20 (Rai et al 2009;Cavello et al 2012;Prakash et al 2010a;Rajput et al 2010). Table 4 presents a number of keratinases that are stimulated by the presence of surfactants and reducing agents which make them ideal candidates for detergent formulation, notably: A. maltophilia BE11-1 (Fang et al 2013).…”

“…However, the use of solid-state fermentation (SSF) has gained prominence as it has a number of advantages over SF, including: lower production expense, smaller water and energy demand, less effluent production and more stable products.Therefore, SSF technology holds a tremendous promise, especially in developing countries ( superparamagnetic nanoparticles showed a fourfold increase in the enzymatic activity over the free enzyme; and enhanced thermal stability, storage stability and recyclability were also observed (Konwarh et al 2009). The thermal stability of the keratinase from…”

Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives

“…(Xu et al, 2014a) Micro and Nanoparticles (Sun et al, 2009) Nanoparticle for medical application Graphene oxide and its derivative in biomaterials (Amieva et al, 2014) As a diet supplement for feeding ruminants (Coward-Kelly et al, 2006) (Dalev, 1994) (Dalev et al, 1996) (Dalev et al, 1997) As a microporous material used as electrode material (Zhan and Wool, 2011) Thermoplastic films for packaging of food ) (Jin et al, 2011 Waste managment using microorganisms in degradation (Vasileva-Tonkova et al, 2009) (Syed et al, 2009) (Grazziotin et al, 2006) Leather processing (Sastry et al, 1986) (Sehgal et al, 1987) (Karthikeyan et al, 2007) Hand spun yan (Reddy and Yang, 2007) Textile yarns (Reddy et al, 2014a) ) ) Keratinases in detergents formulation (Balakumar et al, 2013) (Manivasagan et al, 2014) (Rai et al, 2009) Flame retardant (Wang et al, 2014) Bio-composites or composite fabrication (Flores-Hernández et al, 2014) (Spiridon et al, 2012) (Huda and Yang, 2008) (Gurav and Jadhav, 2013) (Hadas and Kautsky, 1994) (Gousterova et al, 2012) The enzyme keratinases can be used as a fertilizer for de-hairing in leather industry, detergent, feed and other biomedical products. Keratinase can be isolated and purified from poultry feathers (Balakumar et al 2013).…”

“…It is worthy to note that the detergent containing keratinase have good removal power of the blood, paint and dye stains from fabrics. (Rai et al 2009) reported that Bacillus subtilis strain RM-01 was used to generate feathers keratinase having molecular weight 20.1 kDa. He reported maltose and sodium nitrate were the co carbon and co-nitrogen sources.…”