Purification, characterization and antioxidant properties of low molecular weight collagenous polypeptide (37 kDa) prepared from whale shark cartilage (Rhincodon typus)

Elango, Jeevithan; Bao, Bin; Zhang, Jingyi; Hong, Shaotong; Wu, Wenhui

doi:10.1007/s13197-015-1715-5

Cited by 31 publications

(22 citation statements)

References 45 publications

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“…Since the antioxidant properties of marine collagen peptides both from invertebrate and vertebrate sources have been well-documented in the last decade [36,42,50,51,52,53,54,55], we also decided to assess the radical scavenging activity of the four MCH fractions, both in cell-free and cellular in vitro tests to evaluate their potential as antioxidant drugs/supplements. In particular, for the cell-free measurements, two spectrophotometric tests were employed: the DPPH radical scavenging activity test for generic reactive oxygen species (ROS), and the Nitro Blue Tetrazolium (NBT)/riboflavin test measuring superoxide anion scavenging activity (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

et al. 2018

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Recently, the bioactive properties of marine collagen and marine collagen hydrolysates have been demonstrated. Although there is some literature assessing the general chemical features and biocompatibility of collagen extracts from marine sponges, no data are available on the biological effects of sponge collagen hydrolysates for biomedical and/or cosmetic purposes. Here, we studied the in vitro toxicity, antioxidant, wound-healing, and photoprotective properties of four HPLC-purified fractions of trypsin-digested collagen extracts—marine collagen hydrolysates (MCHs)—from the marine sponge C. reniformis. The results showed that the four MCHs have no degree of toxicity on the cell lines analyzed; conversely, they were able to stimulate cell growth. They showed a significant antioxidant activity both in cell-free assays as well as in H2O2 or quartz-stimulated macrophages, going from 23% to 60% of reactive oxygen species (ROS) scavenging activity for the four MCHs. Finally, an in vitro wound-healing test was performed with fibroblasts and keratinocytes, and the survival of both cells was evaluated after UV radiation. In both experiments, MCHs showed significant results, increasing the proliferation speed and protecting from UV-induced cell death. Overall, these data open the way to the use of C. reniformis MCHs in drug and cosmetic formulations for damaged or photoaged skin repair.

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Section: Resultsmentioning

confidence: 99%

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

et al. 2018

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“…Bovine collagen electrospun has been confirmed significant biocompatibility and no cytotoxicity. In continuation to our earlier research [23,24,25,26], in vivo wound healing properties of tilapia collagen sponges and bovine collagen electrospun were studied in rat models in order to evaluate its mechanism on accelerating wound healing properties.…”

Section: Introductionmentioning

confidence: 99%

Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo

et al. 2019

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The development of biomaterials with the potential to accelerate wound healing is a great challenge in biomedicine. In this study, four types of samples including pepsin soluble collagen sponge (PCS), acid soluble collagen sponge (ACS), bovine collagen electrospun I (BCE I) and bovine collagen electrospun II (BCE II) were used as wound dressing materials. We showed that the PCS, ACS, BCE I and BCE II treated rats increased the percentage of wound contraction, reduced the inflammatory infiltration, and accelerated the epithelization and healing. PCS, ACS, BCE I, and BCE II significantly enhanced the total protein and hydroxyproline level in rats. ACS could induce more fibroblasts proliferation and differentiation than PCS, however, both PCS and ACS had a lower effect than BCE I and BCE II. PCS, ACS, BCE I, and BCE II could regulate deposition of collagen, which led to excellent alignment in the wound healing process. There were similar effects on inducing the level of cytokines including EGF, FGF, and vascular endothelial marker CD31 among these four groups. Accordingly, this study disclosed that collagens (PCS and ACS) from tilapia skin and bovine collagen electrospun (BCE I and BCE II) have significant bioactivity and could accelerate wound healing rapidly and effectively in rat model.

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“…Proteases (pepsin, trypsin, and a‐chymotrypsin) and acid hydrolysis are simple and inexpensive methods to digest a protein into short‐chain peptides and free amino acids. Protein hydrolysates from many animals and plants, including fish collagen peptides (Kim et al, ), porcine myofibrillar protein (Saiga, Tanabe, & Nishimura, ), soy protein (Moure, Domínguez, & Parajó, ), acid‐soluble collagen and pepsin‐soluble collagen from the whale shark cartilage (Jeevithan, Bao et al, ) have been found to possess antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Effects of ion concentrations on the hydroxyl radical scavenging rate and reducing power of fish collagen peptides

Shen

Liu

et al. 2019

J Food Biochem

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Empirical evidence proves that the antioxidant property plays a main role in the biological activities of biomaterials, which is influenced by several factors. In order to explore in‐depth, the influence of ionic systems on the antioxidant activity of collagen peptides was studied. Type‐I‐collagen peptides (GBB‐10SP and TYPE‐S) contained a high amount of hydrophobic amino acids and possessed good antioxidant activity at high concentrations in water. On the other hand, increasing [H+] and [Na+] concentrations (0.1 M to 0.5 M) decreased the reducing power of GBB‐10SP and TYPE‐S; however, [Ca2+] had no effect on the reducing power. Interestingly, the hydroxyl radical scavenging rate of these two peptides was increased by [Na+], decreased by [H+], and [Ca2+] had no effect. In general, TYPE‐S showed better antioxidant properties than GBB‐10SP. Overall, the above results confirmed that the antioxidant capacity of collagen peptides was affected by [H+] and [Na+] and not by [Ca2+]. Practical applications The present study mainly deals with the influence of ions on the antioxidant properties of collagen peptides. Recently, fish collagen peptides have been widely used as food supplements to cure several disorders and maintain normal physiological health in humans. It is noted that the use of collagen from fish processing wastes has brought several potential benefits including low value‐added products, preventing environmental pollution, and disease transmission by mammalian‐based collagen. But the biological activity of this peptide varied based on the preparation and its biochemical compositions. Here, we are reporting how to improve the biological activity of collagen, and also the factors affecting the antioxidant properties in order to avoid the down‐regulating mechanism. This study concluded that the [H+] and [Na+] influenced the antioxidant properties of collagen peptides, but [Ca2+] had not effect on the antioxidant properties.

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Purification, characterization and antioxidant properties of low molecular weight collagenous polypeptide (37 kDa) prepared from whale shark cartilage (Rhincodon typus)

Cited by 31 publications

References 45 publications

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo

Effects of ion concentrations on the hydroxyl radical scavenging rate and reducing power of fish collagen peptides

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