Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae

Abstract

In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions—CPP1, CPP2, and CPP3, (CPPs)—were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries.

Water Soluble Antioxidative Crude Polysaccharide From Russula senecis Elicits TLR Modulated NF-κB Signaling Pathway and Pro-inflammatory Response in Murine Macrophages

“…In this context, some researchers have also reported that polymers with more rhamnose and mannose content are able to display better antioxidant activity (Wang et al, 2017). Thus, it could be assumed that the remarkable antioxidant effect of Rusenan was not due to a single element but the result of combination of many factors.…”

Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans

“…Production of GalactoPol and FucoPol isolated from Pseudomonas oleovorans and Enterobacter A47, respectively, are maximized under aerobic conditions, with carbon availability concomitant with nitrogen and oxygen limitation 38 . In contrast, gellan and cellulose production is favored in media supplemented with vitamins and amino acids [39][40][41][42][43][44] . We also investigated the optimal culture condition for DeinoPol production based on its sugar composition analyzed by Bio-LC mass spectrometry in this study.…”

“…We used gradient ethanol precipitation to purify DeinoPol, but its high toxicity (LD 50 = 12.5 μg/mL) presents a limitation for its use as a cosmetic ingredient. We, therefore, used the Sevage method after ethanol precipitation to remove toxic, insoluble materials such as lipids and membrane proteins [41][42][43] . The final product was shown to be non-toxic at over 1000 μg/mL in NHEK-Ad (Supplement ary Fig.…”

“…DeinoPol was extracted, isolated, and purified as described in previous studies [39][40][41][42] with slight modifications. D. radiodurans R1 strain was cultured in 4 L of TGY broth at 30 °C, shaking at 900 rpm.…”

“…The concentrated supernatant (approximately 40 mL) was precipitated with 160 mL of 95% ethanol (Daejungchem; Seoul, Korea) at 4 °C for 12 h, and the precipitate was collected by centrifugation at 5000 × g for 10 min at 4 °C to yield the crude polysaccharide solution. The proteins in the crude polysaccharide were removed by the Sevage method as described previously 41,42 . Briefly, the precipitate was dissolved in 10 mL of distilled water and mixed with 30 mL chloroform:n-butanol (4:1 v/v).…”