Purification, characterization and anticoagulant activity of a proteolytic enzyme from Vespa orientalis venom

Haim, B; Rimon, Abraham; Ishay, Jacob S.; Rimon, Sara

doi:10.1016/s0041-0101(98)00218-9

Cited by 21 publications

(18 citation statements)

References 6 publications

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“…The venom has a proteolytic activity on 14C‐globin, which is partially inhibited by trasylol and ethylenediaminetetraacetic acid. Thus, the venom sac extract exhibits both serine and metaloprotease activities which may affect the activity of the plasma coagulation factors 24,25 . In , there were markedly prolonged coagulation tests (PT and aPTT) and severe thrombocytopenia.…”

Section: Discussionmentioning

confidence: 99%

Wasp envenomation‐induced acute renal failure: A report of three cases (Case Report)

Vikrant¹,

Pandey²,

Machhan³

et al. 2005

Nephrology

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Acute renal failure is an unusual complication of wasp stings. We report three cases who developed acute renal failure after multiple wasp stings (Vespa magnifica). Two patients had evidence of intravascular haemolysis and rhabdomyolysis whereas one patient investigation showed no evidence of intravascular haemolysis or rhabdomyolysis. All three cases had impaired liver functions. Oligo-anuria was seen in all three of the patients and all of them required dialytic support. One patient died of massive gastrointestinal bleeding while the remaining two recovered completely. Although acute renal failure after wasp stings is typically caused by acute tubular necrosis in the setting of haemolysis or rhabdomyolysis, in some patients, renal failure may result from a direct nephrotoxic effect or acute interstitial nephritis from a hypersensitivity reaction to the wasp venom.

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Section: Discussionmentioning

confidence: 99%

Wasp envenomation‐induced acute renal failure: A report of three cases (Case Report)

Vikrant¹,

Pandey²,

Machhan³

et al. 2005

Nephrology

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“…The clotting time in the presence of different concentrations of P. bistriata venom was evaluated with the addition of samples of venom 10 min before the CaCl 2 (0.1 M) and time intervals after addition [27]. …”

Section: Methodsmentioning

confidence: 99%

Biochemical and Functional Characterization ofParawixia bistriataSpider Venom with Potential Proteolytic and Larvicidal Activities

Gimenez

Coutinho-Neto

Kayano

et al. 2014

BioMed Research International

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Toxins purified from the venom of spiders have high potential to be studied pharmacologically and biochemically. These biomolecules may have biotechnological and therapeutic applications. This study aimed to evaluate the protein content of Parawixia bistriata venom and functionally characterize its proteins that have potential for biotechnological applications. The crude venom showed no phospholipase, hemorrhagic, or anti-Leishmania activities attesting to low genotoxicity and discrete antifungal activity for C. albicans. However the following activities were observed: anticoagulation, edema, myotoxicity and proteolysis on casein, azo-collagen, and fibrinogen. The chromatographic and electrophoretic profiles of the proteins revealed a predominance of acidic, neutral, and polar proteins, highlighting the presence of proteins with high molecular masses. Five fractions were collected using cation exchange chromatography, with the P4 fraction standing out as that of the highest purity. All fractions showed proteolytic activity. The crude venom and fractions P1, P2, and P3 showed larvicidal effects on A. aegypti. Fraction P4 showed the presence of a possible metalloprotease (60 kDa) that has high proteolytic activity on azo-collagen and was inhibited by EDTA. The results presented in this study demonstrate the presence of proteins in the venom of P. bistriata with potential for biotechnological applications.

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“…[1][2][3] Anticoagulation factor II (ACF II) is a nonenzymatic anticoagulant isolated from the venom of Agkistrodon acutus, which has marked anticoagulant activity with a unique anticoagulant mechanism: it forms a 1:1 complex with activated coagulation factor X (FXa) in a Ca 2ϩ -dependent fashion and thereby blocks the amplification of the coagulation cascade. 4,5 ACF II has two Ca 2ϩ -binding sites with different affinities for Ca 2ϩ .…”

Section: Introductionmentioning

confidence: 99%

Effects of terbium and pH on structure of anticoagulation factor II from Agkistrodon acutus venom probed by fluorescent spectroscopy and equilibrium dialysis

Xiao

Liu

et al. 2002

Biopolymers

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Anticoagulation factor II (ACF II) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein with marked anticoagulant activity. Present studies show that the pH has a marked effect on the fluorescence intensity of holo-ACF II; however, no appreciable shift of the emission maximum of holo-ACF II was observed in the pH range of 3-10. It was deduced from a relatively weak fluorescence emission of holo-ACF II at a neutral pH (6-7) that native holo-ACF II assumes a compactly folded structure in which the most interior Trp residues and quenchers are adjacent. Terbium ions can completely replace both Ca2+ ions in holo-ACF II as determined by equilibrium dialysis. Two Tb3+-binding sites with different apparent Tb3+ association constant values, (2.1 +/- 0.2) and (1.0 +/- 0.1) x 10(7) M(-1), were identified through Tb3+ fluorescence titration. In addition, it was confirmed from the titration of holo-ACF II and Tb3+-ACF II with N-bromosuccinimide (NBS) that only interior Trp residues are involved in the energy transfer to Tb3+ ions and all accessible Trp residues located in the surface of holo-ACF II have a similar affinity to NBS while those located in the surface of Tb3+-ACF II have two different kinds of affinity to NBS, which suggests a conformational change of holo-ACF II on the substitution of Tb3+ for Ca2+.

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Purification, characterization and anticoagulant activity of a proteolytic enzyme from Vespa orientalis venom

Cited by 21 publications

References 6 publications

Wasp envenomation‐induced acute renal failure: A report of three cases (Case Report)

Wasp envenomation‐induced acute renal failure: A report of three cases (Case Report)

Biochemical and Functional Characterization ofParawixia bistriataSpider Venom with Potential Proteolytic and Larvicidal Activities

Effects of terbium and pH on structure of anticoagulation factor II from Agkistrodon acutus venom probed by fluorescent spectroscopy and equilibrium dialysis

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