Purification, Characterization and Antibacterial Properties of Peptide from Marine Ascidian Didemnum sp.

Arumugam, Velusamy; Venkatesan, Manigandan; Ramachandran, Karthik; Ramachandran, Saravanan; Palanisamy, Satheesh Kumar; Sundaresan, Umamaheswari

doi:10.1007/s10989-019-09829-z

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…AMPs from ascidian Didemnum sp. exhibit an antibacterial effect against human pathogens E. faecalis, S. marcescens, S. typhimurium, and S. aureus at MICs of 2.30, 2.17, 2.05, and 1.95 µg/ml, respectively (Arumugam et al, 2020). The AMPs halocyntin and papillosin from tunicate H. papillosa exhibit antibacterial activity against M. luteus and E. coli (Palanisamy et al, 2017).…”

Section: Antimicrobial Peptides Derived From Marine Invertebratesmentioning

confidence: 99%

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

et al. 2021

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The rapid emergence of multidrug resistant microorganisms has become one of the most critical threats to public health. A decrease in the effectiveness of available antibiotics has led to the failure of infection control, resulting in a high risk of death. Among several alternatives, antimicrobial peptides (AMPs) serve as potential alternatives to antibiotics to resolve the emergence and spread of multidrug-resistant pathogens. These small proteins exhibit potent antimicrobial activity and are also an essential component of the immune system. Although several AMPs have been reported and characterized, studies associated with their potential medical applications are limited. This review highlights the novel sources of AMPs with high antimicrobial activities, including the entomopathogenic nematode/bacterium (EPN/EPB) symbiotic complex. Additionally, the AMPs derived from insects, nematodes, and marine organisms and the design of peptidomimetic antimicrobial agents that can complement the defects of therapeutic peptides have been used as a template.

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Section: Antimicrobial Peptides Derived From Marine Invertebratesmentioning

confidence: 99%

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

et al. 2021

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“…This is also because the marine is considered a prolific source of structurally and functionally diverse MBPs at large, and MBPs in particular. The pharmacological activities, such as antimicrobial, antiviral, anticancer, anticoagulant, antidiabetic, cardioprotective, immunomodulatory, neuroregenerative, appetite suppressing, and many other MBPs, have been described in the literature [ 14 , 15 , 16 , 17 , 18 , 19 , 51 , 52 ]. Anticancer, anti-inflammatory, immunomodulating, antioxidant, hepatoprotective, and neuroprotective activities of phycobiliproteins from cyanobacteria and red algae are reported [ 15 , 53 ].…”

Section: Bioactivities—an Overviewmentioning

confidence: 99%

Exploring Marine as a Rich Source of Bioactive Peptides: Challenges and Opportunities from Marine Pharmacology

et al. 2022

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This review highlights the underexplored potential and promises of marine bioactive peptides (MBPs) with unique structural, physicochemical, and biological activities to fight against the current and future human pathologies. A particular focus is given to the marine environment as a significant source to obtain or extract high-value MBPs from touched/untouched sources. For instance, marine microorganisms, including microalgae, bacteria, fungi, and marine polysaccharides, are considered prolific sources of amino acids at large, and peptides/polypeptides in particular, with fundamental structural sequence and functional entities of a carboxyl group, amine, hydrogen, and a variety of R groups. Thus, MBPs with tunable features, both structural and functional entities, along with bioactive traits of clinical and therapeutic value, are of ultimate interest to reinforce biomedical settings in the 21st century. On the other front, as the largest biome globally, the marine biome is the so-called “epitome of untouched or underexploited natural resources” and a considerable source with significant potentialities. Therefore, considering their biological and biomedical importance, researchers around the globe are redirecting and/or regaining their interests in valorizing the marine biome-based MBPs. This review focuses on the widespread bioactivities of MBPs, FDA-approved MBPs in the market, sustainable development goals (SDGs), and legislation to valorize marine biome to underlying the impact role of bioactive elements with the related pathways. Finally, a detailed overview of current challenges, conclusions, and future perspectives is also given to satisfy the stimulating demands of the pharmaceutical sector of the modern world.

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“…They have antibacterial activity against both Gram-negative and Gram-positive bacteria and are also highly stable at various pH levels and temperatures, which makes them a potential antibiotic alternative. However, Synoicum turgens contain short amounts of this antibacterial peptide, and as the isolation process is complicated, purification is more difficult and large-scale production cannot be achieved [ 30 , 31 ]. It is difficult to synthesize Turgencin A chemically [ 32 ], and the synthesized antimicrobial peptide does not adequately restore the secondary and tertiary structures of the original antimicrobial peptide, meaning the antimicrobial activity of this peptide is significantly reduced.…”

Section: Introductionmentioning

confidence: 99%

The Expression of Antibacterial Peptide Turgencin A in Pichia pastoris and an Analysis of Its Antibacterial Activity

Dong

Zhang

et al. 2023

Molecules

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Antibiotic resistance to pathogenic bacteria is becoming an increasing public health threat, and identifying alternatives to antibiotics would be an effective solution to the problem of drug resistance. Antimicrobial peptides are small peptides produced by various organisms; they are considered to be adequate antibiotic substitutes because they have intense, broad−spectrum antibacterial activity and stability, are widely available, and target strains do not quickly develop resistance. Recent research on antimicrobial peptides has shown that they have broad potential for applications in medicine, agriculture, food, and animal feed. Turgencin A is a potent antimicrobial peptide isolated from the Arctic sea squirt. We established a His-tagged expression system for Pichia pastoris and developed a rTurgencin A using the recombinant expression in Pichia pastoris with nickel column purification. This antimicrobial peptide showed intense antimicrobial activity against Gram-positive and Gram-negative bacteria and a good stability at most temperatures and pHs, as well as in various protease and salt ion concentrations, but underwent a significant decrease in stability in high-temperature and low-pH environments. Turgencin A induced bacterial membrane rupture, resulting in content leakage and subsequent cell death. It was also shown to have low hemolytic activity. This study provides primary data for the industrial production and application of the antimicrobial peptide Turgencin A.

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Purification, Characterization and Antibacterial Properties of Peptide from Marine Ascidian Didemnum sp.

Cited by 8 publications

References 40 publications

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

Exploring Marine as a Rich Source of Bioactive Peptides: Challenges and Opportunities from Marine Pharmacology

The Expression of Antibacterial Peptide Turgencin A in Pichia pastoris and an Analysis of Its Antibacterial Activity

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