Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Carvalho, Aline de Souza; Silva, Márcia Vanusa da; Gomes, Francis Soares; Paiva, Patrícia Maria Guedes; Malafaia, Carolina Barbosa; Silva, Túlio Diego da; Vaz, Antônio Fernando de Melo; Silva, Alexandre Gomes da; Arruda, Isabel Renata de Souza; Napoleão, Thiago Henrique; Carneiro-da-Cunha, Maria das Graças; Correia, Maria Tereza dos Santos

doi:10.1016/j.ijbiomac.2015.02.001

Cited by 34 publications

(7 citation statements)

References 36 publications

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“…These results are comparable to the reported results of lectins purified from Vatairea marcocarpa [11], Vatairea guianensis [15], Platymiscium floribundum [16], and Canavalia oxyphylla [42]. In contrast, extremely thermostable lectins have been reported from Bauhinia forficate [43] and Apuleia leiocarpa [44]. These lectins retain maximum hemagglutinating activity when heated at 100°C.…”

Section: Resultssupporting

confidence: 88%

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

Odekanyin

Akande

2019

JALSI

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Objective: This study was carried out to purify and characterize a carbohydrate-binding and cell-agglutinating protein, lectin, from Pterocarpus osun seeds and also to evaluate its antioxidant and antibacterial potential. Methods: Isolation and purification of the lectin were done by ammonium sulphate precipitation and size exclusion chromatography on Sephadex G-100. Physicochemical properties of the lectin were determined and antioxidant activity was evaluated by DPPH radical scavenging, lipid peroxidation inhibition and ferric reducing antioxidant potential assay. A disc diffusion method was used for antibacterial effect. Results: Lectin was detected in the seeds and was able to agglutinate native and enzyme-treated rabbit erythrocytes but only enzyme-treated erythrocytes of human blood were agglutinated. Mannose, Maltose and α-methylmannoside inhibited the divalent cation-independent hemagglutinating activity, which was stable up to 60°C and at pH range of 3-13. It showed antioxidant activity with IC50 of 1.17 ± 0.08, 0.58 ± 0.03, and 2.51 ± 0.03 mg/ml for those methods respectively. No antibacterial activity was observed. Conclusion: Pterocarpus osun seeds lectin possess properties similar to other lectins from Dalbergieae tribe and its ability to scavenge free radical and inhibit lipid peroxidation show the presence of a valuable health promoting agent in the seeds.

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Section: Resultssupporting

confidence: 88%

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

Odekanyin

Akande

2019

JALSI

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“…ApulSL was also bactericidal against three varieties of X. campestris (Carvalho et al . ). A galactose‐specific lectin from the leguminous Bauhinia monandra secondary roots (BmoRoL) showed antifungal activity on phytopathogenic species of Fusarium , with highest effect against Fusarium solani (Souza et al .…”

Section: Antimicrobial Lectinsmentioning

confidence: 97%

Lectins as antimicrobial agents

et al. 2018

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Summary The resistance of micro‐organisms to antimicrobial agents has been a challenge to treat animal and human infections, and for environmental control. Lectins are natural proteins and some are potent antimicrobials through binding to carbohydrates on microbial surfaces. Oligomerization state of lectins can influence their biological activity and maximum binding capacity; the association among lectin polypeptide chains can alter the carbohydrate–lectin binding dissociation rate constants. Antimicrobial mechanisms of lectins include the pore formation ability, followed by changes in the cell permeability and latter, indicates interactions with the bacterial cell wall components. In addition, the antifungal activity of lectins is associated with the chitin‐binding property, resulting in the disintegration of the cell wall or the arrest of de novo synthesis from the cell wall during fungal development or division. Quorum sensing is a cell‐to‐cell communication process that allows interspecies and interkingdom signalling which coordinate virulence genes; antiquorum‐sensing therapies are described for animal and plant lectins. This review article, among other approaches, evaluates lectins as antimicrobials.

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“…Lectins have been used as pharmacological tools in several areas and have presented antimicrobial [ 9 - 11 ], anti-inflammatory [ 12 - 14 ], antitumor [ 15 - 17 ], antinociceptive [ 18 - 20 ], healing [ 21 - 23 ], and neuroprotective effects [ 24 - 26 ]. Scientific studies with lectins are very relevant as they have several and important applications, constituting one of the most versatile groups of proteins that are used in basic and applied biomedical and biological research.…”

Section: Introductionmentioning

confidence: 99%

Animal Galectins and Plant Lectins as Tools for Studies in Neurosciences

Araújo

Coelho

Campos

et al. 2020

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Lectins are proteins or glycoproteins of non-immunological origin capable of reversibly and specifically binding to glycoconjugates. They exist in free form or associated with cells and are widely distributed in nature, being found in plants, microorganisms, and animals. Due to their characteristics and mainly due to the possibility of reversible binding to glycoconjugates, lectins have stood out as important tools in research involving Neurobiology. These proteins have the ability to modulate molecular targets in the central nervous system (CNS) which may be involved with neuroplasticity, neurobehavioral effects, and neuroprotection. The present report integrates existing information on the activity of animal and plant lectins in different areas of Neuroscience, presenting perspectives to direct new research on lectin function in the CNS, providing alternatives for understanding neurological diseases such as mental disorders, neurodegenerative, and neuro-oncological diseases, and for the development of new drugs, diagnoses and therapies in the field of Neuroscience.

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Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Cited by 34 publications

References 36 publications

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

Lectins as antimicrobial agents

Animal Galectins and Plant Lectins as Tools for Studies in Neurosciences

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