Purification, antioxidant activities, encapsulation, and release profile of total flavonoids in Peony seed meal

Abstract

As potential biomass resources, biomass waste products have been considered worldwide in recent decades. Peony seed meal (PSM) is a kind of agricultural resource waste containing polyphenols, in particular flavonoids. In this study, the total flavonoids of PSM were extracted and purified by AB‐8 macroporous resin (MR), the antioxidant activities of three extract fractions were evaluated, and the total flavonoids were encapsulated with alginate and chitosan by the complex coacervation method. After purification, the yield of total flavonoids was 11.32% and the content in the product increased to 42.89% ± 2.66. The antioxidant activities of three fractions on ·OH, DPPH, and ABTS assays exhibited the following descending order: ethanol elution fraction (ELF) > ethyl acetate extract fraction (EAF) > ethanol extract fraction (EEF). The single‐factor assay showed that the encapsulated total flavonoid microcapsules (EFMs) were prepared with a chitosan concentration of 10 mg/ml, a sodium alginate concentration of 30 mg/ml, a calcium chloride concentration of 50 mg/ml, a ratio of sodium alginate to total flavonoids of 1:3, a flavonoid concentration of 40 mg/ml, and an encapsulation yield of 80.7%. Most microcapsules are smooth‐faced, spherical and uniform in size ranging from 2 to 3 mm in diameter. In vitro release studies suggested that the EFM was stable at pH 1.2 and dissolved at pH 7.5. The result indicated that the EFM is worthy for the development of functional foods and supplements, and PSM could be a potential resource in the food and pharmaceutical industries.

Optimization of Extraction of Compound Flavonoids from Chinese Herbal Medicines Based on Quantification Theory and Evaluation of Their Antioxidant Activity

“…e difference in the hydroxyl radical scavenging rates of TBFs and PCFs was not significant (P > 0.05). CFs showed excellent hydroxyl radical scavenging capability at all concentrations in a dose-dependent manner, which surpassed the scavenging capabilities of tremella flavonoids (lower than 30%) [34], peony seed meal flavonoids (70.33%) [35], kale flavonoids (under 60%) [36], and bear bile grass flavonoids (73.79%) [37]. e reducing power reflects the potential activity of natural products and phytochemicals.…”

Development of Nanostructured Lipid Carrier-Loaded Flavonoid-Enriched Zingiber officinale

“…The flavonoid content was determined using a Varian Cary 50-UV–visible spectrophotometer at a wavelength of 430 nm by using the colorimetric method. The colorimetric approach entails adding sodium nitrite, aluminum chloride, and sodium hydroxide to the sample. , First, a quantity of 1 mm of the material was solubilized in 5 mL of distilled water, as demonstrated by Azieana with slight modification . The sample was introduced into a 0.3 mL solution of sodium nitrite (NaNO 2 ) at a concentration of 5% and incubated for 6 min.…”

Effect of optimized germination technology on polyphenol content and hypoglycemic activity of mung bean

“…The referred method was utilized with some modifications ( 17 , 18 ). The AB-8 macroporous adsorption resin was sealed and soaked in 95% ethanol for 24 h, then loaded into the column, washed with deionized water to completely eliminate the ethanol, then washed with 5% HCl solution and 5% NaOH solution for 3 Bv and soaked for 4 h. Finally, the macroporous resin was washed with deionized water for further experiments.…”