volume 1, issue 3, P100207 2020
DOI: 10.1016/j.xpro.2020.100207
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Abstract: Summary Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a method of purification and use of tRNA for such reactions, focusing on tRNA Arg and its use for arginylation. This method can also be used for other tRNA-mediated reactions. …

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