Purification and Substrate Specificity of endo-Typo Inulinase from Penicillium purpurogenum

Purification and Subsite Affinities ofexo-lnulinase fromPenicillium trzebinskii

“…Inulin (apparent molecular weight of 5000) and sucrose were purchased from Wako Pure Chemical Ind., Ltd. Fn (n = 2-5) were prepared from inulin with endo-inulinase. 16 ) GF n (n =2-8) were isolated from roots of asparagus. 7) DEAE-Sepharose CL-6B (Pharmacia Fine Chemicals), Toyopearl HW-65S (TOSOH Co., Ltd.), and Bio Gel P-150 (Bio-Rad Laboratories) were obtained from commercial sources.…”

“…Recently, we obtained endo-and exo-inulinases from some strains of Penicillium. 14,15) In our previous study, we investigated the substrate specificities of endo-inulinase 16 ) from Penicillium purpurogenum, which hydrolyze inulin and oligosaccharides. From kinetic studies it was demonstrated that the enzyme had a subsite structure in the active site.…”

Structural analysis of six novel oligosaccharides synthesized by glucosyl transfer from β-d-glucose 1-phosphate to raffinose and stachyose using Thermoanaerobacter brockii kojibiose phosphorylase

“…Furthermore, these are known to stimulate selectively the growth of beneficial bacteria such as bifidobacteria, 1 to enhance immune responses, 2 and to promote the absorption of calcium and magnesium. 3 Previously, we have studied the production of nondigestible oligosaccharides as nutritional and functional ingredients such as inulo-oligosaccharide, 4,5 fructooligosaccharide, 6 fructosylxyloside 7 and fructosyllactosucrose 8 using Penicillium purpurogenum inulinase, 4,5 Scopulariopsis brevicaulis fructosyltransferase 6,7 and asparagus 1 F -fructosyltransferase, [8][9][10] respectively. These oligosaccharides were shown to suppress the rise of serum glucose and cholesterol, insulin responses in rats 11 and having prebiotic effects.…”