Purification and Structure Analysis of Antifreeze Proteins from<i>Ammopiptanthus mongolicus</i>

Fei, Y B; Cao, Pengxiu; Gao, Shaopeng; Wang, B; Wei, Lingbo; Zhao, Jiujiang; Chen, G

doi:10.1080/10826060701885126

Cited by 21 publications

(12 citation statements)

References 11 publications

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“…Therefore, the discrepancy between electrophoretic and mass spectrometry data is likely a direct consequence of the structure of the AFP; perhaps it contains modified groups that cannot be extrapolated. The A. nanus AFP was larger than reported for A. mongolicus (Jiang et al 1999;Fei et al 2000), perhaps also due to some modified groups.…”

Section: Discussioncontrasting

confidence: 45%

“…The presence of AFPs is widespread in antifreeze organism, including fish (DeVries and Wohlschlag 1969; Davies and Hew 1990;Evans and Fletcher 2004), plants (Griffith et al 1992;Urrutia et al 1992;Sidebottom et al 2000;AtIcI and Nalbantog˘lu 2003), insects (Zachariassen and Husby 1982; Abbreviations: AFP, antifreeze protein; THA, thermal hysteresis activity; DSC, differential scanning calorimetry; BSA, bovine serum albumin; HDMS TM , high definition mass spectrometry 2004), fungi and bacteria (Duman and Olsen 1993;Gilbert et al 2004). A previous publication by Fei (2000) indicated that AFPs were produced in A. mongolicus leaves during winter, which decreases the freezing temperature of body fluids non-colligatively (Griffith and Yaish 2004). It is not known, however, whether A. nanus also exhibits the same freezing resistant characteristic.…”

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confidence: 99%

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Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus

Yu¹,

Yin²,

Mu³

2010

Can. J. Plant Sci.

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Yu, S., Yin, L. and Mu, S. 2010. Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus. Can. J. Plant Sci. 90: 35Á40. Overwintering plants produce antifreeze proteins (AFPs) that allow plants to survive under freezing or subfreezing conditions. Ammopiptanthus nanus (M. Pop.) Cheng f. grows in the desert of Xinjiang, P.R. China, and our objective was to determine if its survival was dependent on AFP and to identify the protein. Using anion exchange and gel filtration, the AFP was extracted, isolated and purified from cold-acclimated A. nanus leaves. The thermal hysteresis activity (THA) of the antifreeze proteins was measured by differential scanning calorimetry. The THA of the AFP was 0.468C when the concentration of the protein was 20 mg mL (1 . The molecular weight of a band was about 119.24 kDa in SDS-PAGE gel. We detected (PB0.05) the ribuloseÁ1,5-bisphosphate carboxylase/oxygenase large subunit by analyzing the 119.24 kDa protein using the Waters SYNAPT TM high definition mass spectrometry (HDMS TM ) System. Our results indicate that there is an AFP in A. nanus leaves, and that it may play a vital role as a defence against the cold climate, thus increasing the chances for survival of A. nanus in its desert habitat. Ils ont mesure´l'hyste´re´sis thermique (HT) de la prote´ine par calorime´trie diffe´rentielle a`balayage. L'HT de la prote´ine s'e´levait a`0,46 8C, a`une concentration de 20 mg par ml. Le poids mole´culaire d'une des bandes avoisinait 119,24 kDa en e´lectrophore`se sur gel de polyacrylamide en pre´sence de SDS (SDS-PAGE). En analysant la prote´ine de 119,24 kDa avec le syste`me Waters SYNAPT TM HDMS TM , les auteurs ont de´cele´(P B 0,05) la grosse sous-unite´de la ribulose-1,5-biphosphate carboxylase/oxyge´nase. Les re´sultats indiquent donc que les feuilles d'Ammopiptanthus nanus renferment bien une prote´ine antigel qui pourrait jouer un roˆle crucial dans la de´fense de la plante contre le froid et accroıˆtre ses chances de survie dans le de´sert.

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Section: Discussioncontrasting

confidence: 45%

mentioning

confidence: 99%

Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus

Yu¹,

Yin²,

Mu³

2010

Can. J. Plant Sci.

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“…AmEBP1 was able to promote the induction of KIN1, COR15A, and other cold-inducible genes. Because woody perennials must survive long-term freezing temperatures in winter, many need to have developed AFPs and other cold responsive proteins to lower the freezing point of plant issues and to protect plants against frost damage, such as Prunus persica and A. mongolicus (Wisniewski et al 1999;Fei et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Dozens of antifreeze proteins (AFPs) with high thermal hysteresis activity (THA) have been purified and characterized (Fei et al 2008). In this study, our intent was to discover and analyze a battery of genes closely associated with FT in A. Mongolicus.…”

Section: Introductionmentioning

confidence: 99%

Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1

et al. 2008

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In comparison to herbaceous plants, studies of cold responsive genes and cold signaling in woody perennials are limited. Since most woody plants have evolved freezing tolerance (FT) in winter, together with similar lignified structures and winter adaptive mechanisms, it is more likely to find cold resistant genes in woody plants growing in temperate and arctic regions. In this study, Ammopiptanthus mongolicus, an evergreen, broadleaf and leguminous shrub was selected as a model to study cold tolerance in woody plants. Thirteen cold up-regulated cDNAs (including eight full-length cDNAs and five partial cDNAs) were cloned from A. mongolicus. One of these genes, AmEBP1, confers enhanced cold tolerance to E. coli and obvious increased freezing survival to Arabidopsis. In transgenic Arabidopsis expressing AmEBP1, transcript levels of some downstream genes in cold signaling exhibit increased accumulation upon cold treatment. Together with structural information, sub-cellular location, and promoter analysis data, it is suggested that AmEBP1 enhances plants cold tolerance by accelerating ribosome biogenesis and the concomitant translation of cold induced transcription factors and downstream protective proteins under cold stress.

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“…Both samples expressed some other unique structures different from those of fish and insects. It was suggested that plant AFPs might have a particular antifreeze mechanism in comparison with that of fish and insects (Fei et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Biochemical Composition and Antimicrobial Activity of Some Plants in Mongolia

2010

Mong. J. Biol. Sci.

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The antimicrobial activities of the methanol, butanol, ethylacetate, ethanol and chloroform extracts obtained from three different plant species of the Mongolian Gobi desert are evaluated by means of the disc diffusion method against eight species of bacteria. Extracts of Ammopiptanthus mongolicus demonstrated antibacterial activity against three species of microorganisms. Only butanol extract of Incarvillea potaninii demonstrated antibacterial activity against 3-5 species of microorganisms. The methanol and butanol extracts of Gymnocarpos przewalskii demonstrated antibacterial activity against fi ve species. All extracts of three plant species did not demonstrate antibacterial activity against Pseudomonas aeruginosa and Aspergillus niger. In addition, we determined some biologically active substances, pH, vitamin P, raw oil, fl avonoid, alkaloid and anthraglycoside in aboveground parts of these plants.

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Purification and Structure Analysis of Antifreeze Proteins fromAmmopiptanthus mongolicus

Cited by 21 publications

References 11 publications

Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus

Discovery of an antifreeze protein in the leaves of Ammopiptanthus nanus

Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1

Biochemical Composition and Antimicrobial Activity of Some Plants in Mongolia

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