Purification and structural characterization of a novel anti-tumor protein from Arca inflata

Zhu, Jianhua; Xu, Jun; Wang, Yanqing; Li, Chunlei; Chen, Zhiyan; Song, Liyan; Gao, Jingjing; Yu, Rongmin

doi:10.1016/j.ijbiomac.2017.07.001

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…inflata is a traditional marine Chinese medicine that has shown good efficacy in CRC treatment according to historical records in China. In our previous research, we were able to purify two polypeptides with moderate anti-neoplastic activity [15,16]. To further understand the antitumor constituents in A. inflata and their underlying anti-CRC mechanisms, we tracked the molecules that cause antitumor activity using peptide purification coupled with cytotoxicity experiments (Figure 1A).…”

Section: Identification and Physicochemical Properties Of P6mentioning

confidence: 99%

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A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway

Zhang

Zhu

et al. 2022

Marine Drugs

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Colorectal carcinoma (CRC) is one of the major causes of cancer-related incidence and deaths. Here, we identified a novel antitumor peptide, P6, with a molecular weight of 2794.8 Da from a marine Chinese medicine, Arca inflata Reeve. The full amino acid sequence and secondary structure of P6 were determined by tandem mass de novo sequencing and circular dichroism spectroscopy, respectively. P6 markedly inhibited cell proliferation and colony formation, and induced apoptosis in CRC cells. Mechanistically, transcriptomics analysis and a serial functional evaluation showed that P6 induced colon cancer cell apoptosis through the activation of the p38-MAPK signaling pathway. Moreover, it was demonstrated that P6 exhibited antitumor effects in a tumor xenograft model, and induced cell cycle arrest in CRC cells in a concentration-dependent mode. These findings provide the first line of indication that P6 could be a potential therapeutic agent for CRC treatment.

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Section: Identification and Physicochemical Properties Of P6mentioning

confidence: 99%

“…Furthermore, A. inflata is a traditional marine Chinese medicine that has several therapeutic functions, including in cancer treatment. Previously, we identified two antitumor polypeptides and a glucanase from A. inflata [15][16][17]. However, the antitumor mechanism in A. inflata-derived peptides remains insufficiently understood.…”

Section: Introductionmentioning

confidence: 99%

A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway

Zhang

Zhu

et al. 2022

Marine Drugs

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“…For example, a conventional approach is to substitute rare codons by frequent codons according to the genomic codon usage in a host organism (Saito et.al., 2019). In this context, we performed a codon optimization study for J2-C2 gene sequence from A.inflata (Zhu et. al., 2017) on the basis of Escherichia coli.…”

Section: Gene Cloning and Expressionmentioning

confidence: 99%

“…We performed MTT cell viability experiments and determined that the recombinant J2-C2 did not significantly affect cell viability and proliferation of MCF7, PC3 and HT29 ( Figure 2). In studies conducted by Jian Xu et al (2013), it was reported that native J2-C2 isolated and purified from Arca inflata showed antiproliferative effect against A549, SPC-A-1 and HepG2 cell lines (Zhu et. al., 2017).…”

Section: Mtt Cell Viability Assaymentioning

confidence: 99%

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Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells

Songur¹,

Bilgin

Tayhan³

et al. 2020

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Cancer is a big public health problem in many parts of the world. A novel anti-tumor protein (J2-C2) was previously isolated from Arca inflata and it was reported that this protein had antiproliferative effect on some human tumor cell lines such as A549, HepG2 and SPC-A-1. In this study, firstly, J2-C2 was produced by recombinant techniques in the Escherichia coli strain BL21 (DE3) pLysE and this protein was purified by Ni-NTA agarose affinity chromatography. Expressed recombinant J2-C2 was analyzed with SDS-PAGE. 75.5 mg ml-1 of J2-C2 was achieved from a 600 mL culture. Then using HT-29, MCF7 and PC3 cancer cell lines, we showed the effect of recombinant of J2-C2 on cell proliferation, migration and apoptosis in a cell specific manner. Cell viability was measured using MTT assay. Additionally, real-time-qPCR was applied to analyze the transcript levels of apoptosis related genes such as Bcl-2, Bax and p53. The 2-ΔΔCt method was performed to determine the relative changes in gene transcription. Moreover, scratch wound healing assay was performed to evaluate the effect of J2-C2 on cancer cell migration. Consequently, we found that recombinant J2-C2 did not have a significant effect on cell viabilities of MCF7, PC3 and HT29 in concentration-dependent manner. Furthermore, our results showed that recombinant J2-C2 declined HT29, MCF7 cell migration. However, we did not observe the same results for PC3 cancer cell line.

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Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane

Wang,

Turdu,

Zhao

et al. 2024

Chem Nat Compd

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Purification and structural characterization of a novel anti-tumor protein from Arca inflata

Cited by 9 publications

References 39 publications

A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway

A Novel Peptide Derived from Arca inflata Induces Apoptosis in Colorectal Cancer Cells through Mitochondria and the p38 MAPK Pathway

Investigation of Anti-proliferative and Anti-migratory Properties of Recombinant J2-C2 Against Tumor Cells

Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane

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