Purification and Structural Analysis of the Hepatitis B Virus PreS1 Expressed from Escherichia coli

Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy

“…1A, lane 3). Similar to the previous study (11), Western blot analysis with anti-preS1 monoclonal antibody indicated a major band of a 39 K protein corresponding to the intact GSTPreS1, several bands of lower molecular weight proteins that most likely represent degradation products of the GST-preS1 fusion protein (Fig. 1B, lane 3).…”

Multiple hTAFII31-binding motifs in the intrinsically unfolded transcriptional activation domain of VP16

“…Such a pattern was observed in other MU type IUPs that contain more than one pre-structured motifs (4,29). Typically, the population of transient pre-structured motifs in a mostly unstructured type of IUPs is less than 20% (4,31). In p53 TAD where the population of transient motifs is ∼10%, the CD spectral change at 220 nm caused by destruction of pre-structured motifs due to addition of denaturants could be clearly measured (28).…”

Broadly neutralizing anti‐HBV antibody binds to non‐epitope regions of preS1

“…The concentrationdependent increase of RU signals clearly confirms that there are specific interactions between the Fab and all the epitope-near preS1 regions. The interactions of these four preS1 peptides for the Fab were weak with the equilibrium dissociation constant (K d ) values of 1-2 mM range: 1.54, 1.63, 2.04, and 1.99 mM for preS1 (1-11), preS1 (27)(28)(29)(30)(31)(32)(33)(34)(35), preS1 (66-76), and preS1 (95-104), respectively. Although this binding affinity seems low, it is comparable to the affinities observed for germline antibody binding [28] and TCR-peptide/MHC (pMHC) interactions [35].…”

“…HzKR127 Fab in 25 mM sodium acetate, pH 5.5 was immobilized to a channel of CM5 sensor chip using an amine coupling kit (BIAcore AB) and the control flow-subtracted sensograms are obtained. The peptide concentrations range from 10 to 640 nM for preS1 (36-46), from 5 to 80 nM for preS1 (1-119), and from 100 to 800 lM for preS1 (1-11), preS1 (27)(28)(29)(30)(31)(32)(33)(34)(35), preS1 (66-76), and preS1 (95-104). Kinetic measurements were made and kinetic constants were derived with the BIAevaluation Version 3.0 software (BIAcore AB).…”