Purification and some properties of α-amylase from an ectomycorrhizal fungus, Tricholoma matsutake

Kusuda, Mizuho; Nagai, Masaru; Hur, Tae-Chul; Terashita, Takao; Ueda, Mitsuhiro

doi:10.1007/s10267-003-0116-1

Cited by 22 publications

(27 citation statements)

References 12 publications

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“…It has been determined that the enzymatic activities of different amylases occur at pH levels that range from 4.5 to 8.5 (Tabatabai, 1994). In a study made by Kusuda et al (2003), an a-amylase was purified from cultures of the ectomycorrhizal Tricholoma matsutake (which is not phylogenetically related to Descolea) made at 24ºC. This enzyme is active at pH 6, and is stable within the broad range of pH levels from 4 to 10.…”

Section: Discussionmentioning

confidence: 99%

Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

Valenzuela¹,

Hipp²,

Alonso³

et al. 2008

Electron. J. Biotechnol.

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Section: Discussionmentioning

confidence: 99%

Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

Valenzuela¹,

Hipp²,

Alonso³

et al. 2008

Electron. J. Biotechnol.

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“…One unit of α-glucosidase activity was defined as the activity that forms 1 µmol p-nitrophenol in a 1.0-ml reaction mixture at 37°C for 1 min. More detailed procedures are described in our previous paper (Kusuda et al 2003).…”

Section: Measurement Of Amylase Activitiesmentioning

confidence: 99%

“…The culture bottle was autoclaved at 121°C for 60 min before use. After inoculation (Kusuda et al 2003), the vegetative mycelia were grown at 24°C under continuous illumination (about 200 lux) for 60 days. Then, the induction of fruit-body formation was carried out by adjusting the temperature to 15°C, and the medium surface was covered with peat moss (about 5 mm thick).…”

Section: Medium Compositions and Culture Conditionsmentioning

confidence: 99%

Characterization of extracellular glucoamylase from the ectomycorrhizal mushroom Lyophyllum shimeji

et al. 2004

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To investigate the function of amylases in the fruit-body formation of an ectomycorrhizal fungus, Lyophyllum shimeji, we purified the extracellular amylase in the medium of this fungus. The purified enzyme was obtained from 1.7 l stationary culture filtrate, with 4.2% recovery, and showed a single protein band on SDS-PAGE. The molecular mass was about 25 kDa. The enzyme was most active at around 40°C and pH 5.0 and stable over pH 4.5-6.5 for 30 min at 37°C. This amylase was remarkably activated by the presence of Ca 2ϩ ion (7.7 times that of the control), but Ba 2ϩ and Ag ϩ completely inhibited the activity. The amylase readily hydrolyzed the α-1,4 glucosidic linkage such as dextrin and amylose A (MW, 2900), converting into glucose, and hydrolyzed the α-1,6 glucosidic linkage of isomaltohexaose and amylopectin. However, the enzyme did not hydrolyze the cyclic polysaccharides. On the other hand, when a low molecular mass amylose A was hydrolyzed by this amylase, -anomer glucose was produced. From these results, we concluded that the amylase from L. shimeji seems to be a glucoamylase.

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“…Principal hosts include Pinus densiflora, Pinus thunbergii, and Pinus taiwanensis Hayata. However, Quercus aquifolioides and Quercus pannosa are the principal hosts in Sichuan Province [5][6][7][8][9]. Nonetheless, several forms of T. matsutake, such as the matsutake harvested from Xiaojin in Sichuan Province, are frequently found in mixed forests in association with Quercus.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and RNA-seq Transcriptome Analysis of Tricholoma matsutake from Sichuan Province, China

Ding¹,

Wang²,

Hou³

et al. 2016

Pol. J. Environ. Stud.

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The sequences of the internal transcribed spacer (ITS) regions of ectomycorrhizal fungi collected from Sichuan Province were analyzed using a PCR primer pair specific to T. matsutake. The amplified fragments were sequenced and compared with each other to build a phylogenetic tree. The mRNA deep sequencing approach was adopted to identify differentially expressed T. matsutake genes among the transcriptomes developed from a Xiaojin sample. A phylogenetic analysis of the aligned sequences was performed using maximum-likelihood (ML) and neighbor-joining (NJ) analyses. The results clearly showed that the KD (KM657344) and BT (KM657342) strains were more closely related to each other than to other strains. Moreover, T. matsutake from Sichuan differed from those specimens derived from Heilongjiang, Yunnam, and Guizhou provinces of China, Finland, and Japan. Furthermore, there was extremely high homology among these T. matsutake samples, despite some genetic variation. In addition, the genome of T. matsutake was sequenced using Illumina sequencing technology (RNA-seq). In all, a total of 24,549,990 reads were obtained that yielded 18,266,492 high-quality clean reads. The quality reads were excluded later. The BLAST analysis of the sequence reads against the NR database indicated that T. matsutake shared a high number of contigs with Laccaria bicolor. The results also indicated that catalytic activity, metabolic processes, metabolic pathways, and biosynthesis of secondary metabolites were the main functions identified by gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Phylogenetic analysis showed that T. matsutake growing in Sichuan differed from samples growing in other regions. The differences in secondary metabolites between the Sichuan and Xiaojin samples may be due to differences in metabolic pathways. Thus the study provides a foundation for understanding T. matsutake biogeography and

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Purification and some properties of α-amylase from an ectomycorrhizal fungus, Tricholoma matsutake

Cited by 22 publications

References 12 publications

Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

Characterization of extracellular glucoamylase from the ectomycorrhizal mushroom Lyophyllum shimeji

Genetic Diversity and RNA-seq Transcriptome Analysis of Tricholoma matsutake from Sichuan Province, China

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