Purification and some physicochemical properties of bovine κ-casein

Vreeman, H. J.; Both, P.; Brinkhuis, Jan; Spek, Corry Van Der

doi:10.1016/0005-2795(77)90044-7

Cited by 68 publications

(40 citation statements)

References 16 publications

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“…The amino acid composition is identical in all these fractions and they seem to differ only by the amount of carbohydrate i. e. neuraminic acid (NA). Following Vreeman et al [10] the casein can be fractionated in a preparative scale according to the different charges. The components are separated on a DEAEanion exchange column applying a linear pH gradient (see fig.…”

Section: Preparation Of Kappa-caseinmentioning

confidence: 99%

Dynamic light scattering from spherical particles

Kunz

Thurn

Burchard

1983

Colloid & Polymer Sci

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Abstract:The dynamic light scattering behavior of poly(butylmethacrylate) PBMA microgels and of kappa-casein micelles is compared with that from hard sphere latex particles. The latex particles and the kappa-casein micelles exhibited a single exponential decay of the time correlation function (TCF). For the microgels, progessively stronger deviations from a single exponential were observed as the scattering angle was made larger. These deviations are interpreted as being the result of internal modes of motion. From measurement of the first cumulant of the TCF, extrapolated towards zero angle, the translational diffusion coefficients D were determined, and the hydrodynamically effective radii were calculated via the Stokes-Einstein relationship. The ratio of the radius of gyration to the hydrodynamic radius was found to be.Q = 0.775+0.012 for the latex particles, in good agreement with theory. The microgels, however, exhibit much lower Oparameters of 0.49 to 0.58, while the kappa-casein micelles showed the opposite behavior with values between 1.1 and 2.5. The results are interpreted on the basis of the DebyeBueche and Deutsch-Felderhof theory for porous spheres.

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Section: Preparation Of Kappa-caseinmentioning

confidence: 99%

Dynamic light scattering from spherical particles

Kunz

Thurn

Burchard

1983

Colloid & Polymer Sci

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“…However, some of K-casein subcomponents obtained from another cow showed ·the phosphate content to be near 2 mol/mol (data not shown) as reported' by Vreeman etal. 11) Amino acid compositions of the individual subcomponents obtained under the GalNAc determination conditions were very close each other (data not shown).…”

Section: Chemical and Immunological Analyses Oj' Kcasein A Subcomponentsmentioning

confidence: 58%

Fractionation and Characterization of 9 Subcomponents of Bovine κ-Casein A

Takeuchi

Tsuda

Yoshikawa

et al. 1985

Agricultural and Biological Chemistry

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“…Otros estudios realizados en la misma especie (Trujillo et Rojas et al, 2009) donde se utilizaron el mismo par de cebadores. Esta técnica muestra ventajas frente a otros métodos empleados para la selección de ganado como puede ser la prueba de progenie (evaluación del individuo en base a la producción de las hijas) (FAO, 1981; Ochoa, 1991) o el estudio del perfil electroforético de las proteínas lác-teas (Vreeman et al, 1977). En el caso de las pruebas de progenie, seleccionar toros con el genotipo deseado (κ-CN BB) demandaría entre 6 y 7 años (Medrano y Aguilar-Córdova, 1990; Azevedo et al, 2008), lo cual hace poco práctico establecer programas de selección conducentes a incrementar la frecuencia génica del alelo deseado en la población.…”

Section: Discussionunclassified

Genotipos del Gen Kappa-Caseína en Ganado Bovino Criollo del Distrito de Bambamarca, Cajamarca, Perú

Almeyda

Rosadio

Maturrano

2016

Rev. investig. vet. Perú

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RESUMENEl presente estudio tuvo como objetivo determinar la frecuencia alélica y genotípica del gen kappa-caseína (κ-CN) en ganado bovino criollo. Se recolectaron 48 muestras de sangre de bovinos de nueve centros poblados del distrito de Bambamarca (Cajamarca, Perú). Se extrajo el ADN y la genotipificación se realizó mediante análisis de RFLP-PCR del gen κ-CN. Las frecuencias genotípicas encontradas fueron de 0.56, 0.27 y 0.17 para los genotipos AB, AA y BB, respectivamente, en tanto que las frecuencias alélicas resultaron ser de 0.552 y 0.448 para los alelos A y B, respectivamente. Se concluye que los animales evaluados presentan una baja proporción de individuos con el genotipo deseado BB, genotipo que favorece la producción de queso. Palabras clave: bovino criollo, gen kappa-caseína, PCR-RFLP ABSTRACTThe aim of this study was to determine the allelic and genotypic frequency of kappacasein gene (κ-CN) in creole cattle. For this purpose, 48 blood samples were collected from animals in nine villages of the Bambamarca district, Cajamarca, Peru. DNA was extracted and genotyping was performed by PCR-RFLP analysis of κ-CN gene. Genotype frequencies of 0.56, 0.27 and 0.17 for the AB, AA and BB genotypes were found, while the allele frequencies were 0.552 and 0.448 for alleles A and B respectively. It was concluded that the tested group had a low proportion of individuals with the desired BB genotype that favors the production of cheese.

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Purification and some physicochemical properties of bovine κ-casein

Cited by 68 publications

References 16 publications

Dynamic light scattering from spherical particles

Dynamic light scattering from spherical particles

Fractionation and Characterization of 9 Subcomponents of Bovine κ-Casein A

Genotipos del Gen Kappa-Caseína en Ganado Bovino Criollo del Distrito de Bambamarca, Cajamarca, Perú

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