Purification and Properties of the Second Antibacterial Substance Produced by
            <i>Penicillium notatum</i>

Kocholaty, Walter F.

doi:10.1126/science.97.2512.186

Cited by 24 publications

(10 citation statements)

References 1 publication

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“…This enzyme has been produced by various fungal sources, mainly from the genus Aspergillus [8,9] and Penicillium [10,11]. GOD was widely used as a potent antibacterial and antifungal agent in the food industry [12][13][14]. It is capable of inhibiting the growth of microorganisms through naturally produced hydrogen peroxide and gluconic acid [15].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Kriaa

Hammami

Sahnoun

et al. 2015

Comptes Rendus. Biologies

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The present study focuses on the potential of glucose oxidase (GOD) as a promising biocontrol agent for fungal plant pathogens. In fact, a new GOD producing fungus was isolated and identified as an Aspergillus tubingensis. GOD (125 AU) has been found to inhibit Fusarium solani growth and spore production. Indeed, GOD caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. In vivo assays, GOD acted as a curative treatment capable of protecting the tomato plants against F. solani diseases. In fact, the incidence was null in the curative treatment with GOD and it is around 45% for the preventive treatment. The optimization of media composition and culture conditions led to a 2.6-fold enhancement in enzyme activity, reaching 81.48U/mL. This study has demonstrated that GOD is a potent antifungal agent that could be used as a new biofungicide to protect plants from diseases.

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Section: Introductionmentioning

confidence: 99%

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Kriaa

Hammami

Sahnoun

et al. 2015

Comptes Rendus. Biologies

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“…Although the wide‐ranging antibacterial activity of GOX produced by P. notatum (penatin or notatin) was reported more than 60 years ago (Coulthard et al. 1942; Kocholaty 1942,1943) no detailed study on the antifungal activity of Penicillium GOX has been performed thus far, to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

Penicillium chrysogenum glucose oxidase - a study on its antifungal effects

et al. 2004

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Aims: Purification and characterization of the high molecular mass Candida albicans-killing protein secreted by Penicillium chrysogenum. Methods and Results: The protein was purified by a combination of ultrafiltration, chromatofocusing and gel filtration. Enzymological characteristics [relative molecular mass (M r ) ¼ 155 000, subunit structure a 2 with M r,a ¼ 76 000, isoelectric point (pI) ¼ 5AE4] were determined using SDS-PAGE and 2D-electrophoresis. N-terminal amino acid sequencing and homology search demonstrated that the antifungal protein was the glucose oxidase (GOX) of the fungus. The enzyme was cytotoxic for a series of bacteria, yeasts and filamentous fungi. Vitamin C (1AE0 mg ml )1 ) prevented oxidative cell injuries triggered by 0AE004 U GOX in Emericella nidulans cultures but bovine liver catalase was ineffective even at a GOX : catalase activity ratio of 0AE004 : 200 U. A secondary inhibition of growth in E. nidulans cultures by the oxygen-depleting GOX-catalase system was likely to replace the primary inhibition exerted by H 2 O 2 . Conclusions: Penicillium chrysogenum GOX possesses similar enzymological features to those described earlier for other Penicillium GOXs. Its cytotoxicity was dependent on the inherent antioxidant potential of the test micro-organisms. Significance and Impact of the Study: Penicillium chrysogenum GOX may find future applications in glucose biosensor production, the disinfection of medical implants or in the food industry as an antimicrobial and/or preservative agent.

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“…Several different media were used in the serial dilution methods. The kinds of media used in testing (when given in the original publications) are as follows: Streak-plate method (104,115,145,146,160,162,164,166,170). Serial dilution method in (a) beef extract, small inocula (96,102), and large inocula (158); (b) beef heart broth (119,122), small inocula (112,177), and large inocula (50,71,113);…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…Notatin (56), also known as penicillin A, penicillin B (22), and penatin (104). Flavoprotein enzyme, buff -colored powder, absorption maxima a t 2800, 3750, 4650 & (56) ; soluble in water, insoluble in organic Mycophenolic Acid.…”

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confidence: 99%

Antibacterial Substances from Fungi and Green Plants

Kavanagh¹

1947

Advances in Enzymology - And Related Areas of Molecular Biology

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Purification and Properties of the Second Antibacterial Substance Produced by Penicillium notatum

Cited by 24 publications

References 1 publication

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Penicillium chrysogenum glucose oxidase - a study on its antifungal effects

Antibacterial Substances from Fungi and Green Plants

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