Purification and properties of the phytase from Schwanniomyces castellii

“…DS11 (70°C) (Kim et al, 1998). Among yeasts, Schwanniomyces castellii showed maximum phytase activity at 77°C (Segueilha et al, 1992), while Arxula adeninivorans and Pichia spartae at 75 to 80°C, and Pichia rhodanensis at 70 to 75°C (Nakamura, 2000). Phytase of Aerobacter aerogenes exhibited the lowest optimum temperature at 25°C (Greaves et al, 1967).…”

Section: Discussionmentioning

confidence: 99%

“…The activity declined significantly above pH 5.5. The moderately acidic pH optimum of M. jalaludinii phytase indicates that this enzyme belongs to the acidic phytases, as are most of the so far characterized phytases of microorganisms: Selenomonas ruminantium JY35, pH 4.0 to 5.5 (Yanke et al, 1999); E. coli, pH 4.5 (Greiner et al, 1993); Aerobacter aerogenes, pH 4 to 5 (Greaves et al, 1967); Klebsiella aerogenes, pH 4.5 and 5.2 (Tambe et al, 1994); Lactobacillus amylovorus, pH 4.4 (Sreeramulu et al, 1996); Aspergillus terreus, pH 4.5 (Yamada et al, 1968); Schwanniomyces castellii, pH 4.5 (Segueilha et al, 1992); and all yeast strains studied by Nakamura et al( 2000), pH 3 to 5.5. These pH optima are different from those of other bacterial phytases, such as pH 6.5 for Bacillus subtilis (natto) N-77 (Shimizu, 1992), pH 7.0 for Bacillus subtilis VTT E-68013 (Kerovuo et al, 1998) and Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of a phytase from Mitsuokella jalaludinii, a bovine rumen bacterium

Lan¹,

Abdullah²,

Jalaludin³

et al. 2011

Afr. J. Biotechnol.

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The phytase from Mitsuokella jalaludinii, a novel phytase-producing rumen bacterium, was purified 120-fold to near homogeneity and characterized. The phytase was completely cell-associated and about half of the enzyme activity was released when the bacterial cells were incubated with 1.5 mol/l KCl solution for 8 h. The optimum pH for phytase activity was in the range of 4.0 to 5.0 and the optimum temperature was 55 to 60°C. The phytase was stable at pH 4.0 to 7.0. . The metal ions chelating agents, namely trisodium citrate, potassium sodium tartrate and EDTA, did not show any inhibitory effect on the phytase activity of M. jalaludinii. The phytase was also not inhibited by sulfhydryl inhibitor, 2-mercaptoethanol, and a carboxyl inhibitor, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC).

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“…Em virtude da habilidade de formar complexos insolúveis com importantes minerais como Ca, Zn e Mg e também com proteínas, o ácido fítico é considerado um constituinte antinutricional, que reduz a biodisponibilidade desses minerais (Maga, 1982). Assim, o uso da fitase seria uma alternativa para aumentar o valor nutricional de muitos alimentos vegetais (Segueilha et al, 1992). …”

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Caracterização parcial e utilização da fitase extraída de sementes germinadas de girassol

Agostini

Ida

2006

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi ativar, extrair e caracterizar parcialmente a fitase em sementes germinadas de girassol (Helianthus annuus L.), híbrido M734, e avaliar o efeito da fitase no farelo de girassol. Sementes foram colocadas para germinar por oito dias em câmara a 25°C. A fitase foi extraída com CaCl 2 2%, depois do quinto dia de germinação, e fracionada com (NH 4 ) 2 SO 4 até 80% de saturação. O extrato bruto foi caracterizado parcialmente e aplicado em farelo de girassol desengordurado para avaliar a hidrólise do fitato. Com a germinação, houve aumento na atividade da fitase e redução no teor de fitato. A maior atividade da fitase foi observada do quinto ao oitavo dia de germinação. A fitase das sementes germinadas aos cinco dias apresentou atividade ótima em pH de 5,2 e temperatura de 55ºC. A enzima se manteve estável, quando pré-aquecida por 10 minutos a 50ºC, com V máx de 1,87 U g -1 de amostra e K m de 0,29 mM, indicando alta especificidade pelo fitato. Quando aplicada no farelo de girassol desengordurado, depois de oito horas de incubação, a fitase hidrolisou 92% do fitato. A germinação de sementes de girassol estimula a atividade da fitase, o que facilita sua extração para a produção de alimentos livres de fitatos.Termos para indexação: Helianthus annuus, girassol híbrido, hidrólise de fitato. Partially characterization and application of phytase extracted from germinated sunflower seedsAbstract -The objective of this work was to activate, extract and partially characterize the phytase in hybrid sunflower M734 (Hellianthus annuus). The enzyme was extracted from seeds germinated for eight days, with CaCl 2 2%, fractionated with (NH 4 ) 2 SO 4 up to 80% saturation. The obtained crude extract was partially characterized and applied over defatted sunflower flour in order to evaluate the phytate hydrolysis. In germination, there was an increase in the phytase activity and a reduction of phytate content. The highest enzyme activity was observed from the 5 th to the 8 th day of germination. The phytase extracted from sunflower seeds at the 5 th day of germination presented optimum activity at pH 5.2 and temperature at 55ºC. The phytase was heat stable at 50ºC for 10 minutes, and presented V max of 1.87 U g -1 of substract and K m of 0.29 mM, indicating high specificity to phytate. When applied over defatted sunflower flour, after eight hours of incubation the phytase hydrolyzed 92% of phytate. Sunflower seeds germination stimulates phytase activity, making easy the extraction process, in the production of phytate-free feed products.Index terms: Helianthus annuus, sunflower hibrid, phytate hydrolysis. IntroduçãoAs fosfatases são enzimas amplamente distribuídas na natureza que hidrolisam ésteres fosfatos. A fitase, ou mioinositol-hexafosfato fosfohidrolase, é uma fosfatase fitatoespecífica que catalisa a hidrólise do ácido fítico, ou mioinositol hexafosfato, formando inositol e fosfatos. O ácido fítico é a principal forma de armazenamento de P em sementes e pólen (Reddy et al., 1982). Em virtude d...

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Purification and properties of the phytase from Schwanniomyces castellii

Cited by 137 publications

References 8 publications

Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase

Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase

Purification and characterization of a phytase from Mitsuokella jalaludinii, a bovine rumen bacterium

Caracterização parcial e utilização da fitase extraída de sementes germinadas de girassol

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