Purification and properties of mutanase from Bacillus circulans

Cloning and Expression of an α-1,3-Glucanase Gene fromBacillus circulansKA-304: The Enzyme Participates in Protoplast Formation ofSchizophyllum commune

“…RM1 enzyme. Many bacterial -1,3-glucanases, such as those of Flavobacterium sp., 15) Pseudomonas sp., 18) Streptomyces chartreuses, 19) Bacteroides oralis, 20,21) and Bacillus circulans, 22) have been studied from the same point of view as that for the Bacillus sp. RM1 enzyme to remove tooth plaque created by oral bacteria, but the gene of these enzymes has not been cloned, except for that of the Bacillus sp.…”

“…RM1 -1,3-glucanase was named as mutanase (EC.3.2.1.59) that hydrolyzes mutan formed by an oral bacterium, Streptococcus mutans. The presence of mutanases has been also reported in several bacteria [14][15][16][17][18][19][20][21][22] and filamentous fungi. [23][24][25][26][27][28][29][30][31][32][33] Among these -1,3-glucanases, the genes of y To whom correspondence should be addressed.…”

Production and use of mutanase from Trichoderma harzianum for effective degradation of streptococcal mutans

“…Correspondingly, bacterial mutanases from B. circulans (19), Flavobacterium (6) and Microbispora rosea (4) have pH optima similar (5.5) or higher (6.3-6.9) than fungal mutanases, and similar temperature optima Table 5. Characteristics of the non purified mutanase preparation from T. harzianum.…”

“…Mutanases from various bacteria and filamentous fungi have been investigated; yet, in most cases, the enzyme activity is relatively low, and cultural conditions for mutanase production have not been thoroughly established (9,10,19). Also, search for additional mutanase producers among wild-type fungi is *Corresponding Author.…”

Domain Structure and Function of α-1,3-Glucanase fromBacillus circulansKA-304, an Enzyme Essential for Degrading Basidiomycete Cell Walls

“…These enzymes are classified into two families, 71 and 87, of glycoside hydrolases based on their amino acid sequences, referred to in the Carbohydrate Active enZYmes (CaZy) Database. Type 71 -1,3-glucanases have been found in fungi, such as Trichoderma, 3) Penicillium, 3) Aspergillus, 4) and Schizosaccharomyces, 5) and type 87 enzymes have been found in Bacillus 6) and Paenibacillus. 7) These enzymes have been investigated not only to determine their physiological roles in these organisms, 4,5) but also for application in degrading dental plaques, 8,9) controlling pathogenic fungi, 10) preparing fungal protoplasts, 11) and for other uses.…”