Purification and properties of an ice-nucleating protein from an ice-nucleating bacterium, Pantoea ananatis KUIN-3

Muryoi, Naomi; Matsukawa, Kanji; Yamade, Kazuhiro; Kawahara, Hidehisa; Obata, Hitoshi

doi:10.1016/s1389-1723(03)80122-6

Cited by 14 publications

(8 citation statements)

References 27 publications

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“…[1][2][3] INPs are integrated to the outer cell membrane of Gram-negative bacteria where they form large multimers, [4][5][6] or can be released into the surrounding environment known as extracellular ice nucleating material. 7,8 Generally, ina C bacteria are Gram-negative, epiphytic and pathogenic as Pseudomonas syringae, Pseudomonas putidia, Erwinia herbicola, Erwinia ananas, Xanthomonas campestris, or Pantoea ananatis among others. [7][8][9][10][11][12][13][14][15][16][17] The well characterized plant pathogen Pseudomonas syringae represents one of the most efficient INA bacterial ice nucleus known, initiating plant damaging ice formation at temperatures of ¡2 C. 11,18 Aside from the habitat of an epiphytic plant pathogen, Pseudomonas syringae, as well as other INA bacteria were found in clouds, rain, snow and streams indicating that they are disseminated with the earth hydrological cycle.…”

Section: Introductionmentioning

confidence: 99%

“…7,8 Generally, ina C bacteria are Gram-negative, epiphytic and pathogenic as Pseudomonas syringae, Pseudomonas putidia, Erwinia herbicola, Erwinia ananas, Xanthomonas campestris, or Pantoea ananatis among others. [7][8][9][10][11][12][13][14][15][16][17] The well characterized plant pathogen Pseudomonas syringae represents one of the most efficient INA bacterial ice nucleus known, initiating plant damaging ice formation at temperatures of ¡2 C. 11,18 Aside from the habitat of an epiphytic plant pathogen, Pseudomonas syringae, as well as other INA bacteria were found in clouds, rain, snow and streams indicating that they are disseminated with the earth hydrological cycle. [19][20][21][22] Airborne ice-active bacteria are involved in cloud condensation -acting as cloud condensation nuclei (CCN) -and precipitation.…”

Section: Introductionmentioning

confidence: 99%

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Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Kassmannhuber

Rauscher²,

Schöner³

et al. 2017

Bioengineered

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In a concept study the ability to induce heterogeneous ice formation by Bacterial Ghosts (BGs) from Escherichia coli carrying ice nucleation protein InaZ from Pseudomonas syringae in their outer membrane was investigated by a droplet-freezing assay of ultra-pure water. As determined by the median freezing temperature and cumulative ice nucleation spectra it could be demonstrated that both the living recombinant E. coli and their corresponding BGs functionally display InaZ on their surface. Under the production conditions chosen both samples belong to type II ice-nucleation particles inducing ice formation at a temperature range of between ¡5.6 C and ¡6.7 C, respectively. One advantage for the application of such BGs over their living recombinant mother bacteria is that they are non-living native cell envelopes retaining the biophysical properties of ice nucleation and do no longer represent genetically modified organisms (GMOs).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Kassmannhuber

Rauscher²,

Schöner³

et al. 2017

Bioengineered

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“…This suggests that INA type is a reflection of culture conditions, cell cycle phase or INP glycosylation and anchoring (Pooley and Brown, 1991; Ruggles et al ., 1993; Gurian‐Sherman and Lindow, 1995; Cochet and Widehem, 2000). INA has been described in several plant pathogens and epiphytic bacteria (Tegos et al ., 2000) including several species of Pseudomonas ( P. syringae , P. fluorescens, P. viridiflava , P. antarctica and P. putida ), Xanthomonas campestris , X. ananas, Erwinia herbicola and E. uredovora ( Pantoea ananatis ) (Yankofsky et al ., 1997; Xu et al ., 1998; Kawahara, 2002; Yamashita et al ., 2002; Muryoi et al ., 2003; 2004).…”

Section: Introductionmentioning

confidence: 99%

Ice‐active characteristics of soil bacteria selected by ice‐affinity

Wilson

Kelley

Walker

2006

Environmental Microbiology

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As an initial screen for microorganisms that produce ice-active macromolecules, ice-affinity was used to select microorganisms from soil consortia originating from three temperate regions. Once selected and subsequently purified to single colonies, these microbes were putatively identified by 16S ribosomal RNA sequencing and assayed for various ice-active properties. Ice-affinity selection appeared to select for bacteria with ice-associating activities: inhibition of ice recrystallization; ice nucleation; ice shaping. Although none of these activities were observed in Paenibacillus amyloliticus C8, others such as Chryseobacterium sp. GL8, demonstrated both ice recrystallization inhibition and ice-shaping activities. Pseudomonas borealis DL7 was classified as a type I ice nucleator, Flavobacterium sp. GL7, was identified as a type III ice nucleator and Acinetobacter radioresistens DL5 demonstrated ice recrystallization inhibition. In all, 19 different culturable bacteria were selected from the thousands of microbes in late-summer collected soil samples. Many of the selected microbes have been previously reported in glacial ice cores or polar sea ice, and of five isolates that were further characterized, four showed ice-associating activities. These results indicate the significant potential of ice-affinity selection even with temperate climate soils, suggesting that sampling in more extreme and remote areas is not required for the isolation of ice-active bacteria.

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“…INP is a membrane-bound protein, and purified INP had extremely low ice-nucleation activity (Muryoi et al, 2003). Lindow (1995) showed that INP activity in P. syringae was associated with membrane fluidity.…”

Section: Resultsmentioning

confidence: 99%

High saturated fatty acids proportion inEscherichia colienhances the activity of ice-nucleation protein fromPantoea ananatis

Liu

Tao

et al. 2013

FEMS Microbiol Lett

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The ice-nucleation protein (INP) from Pantoea ananatis was expressed in Escherichia coli. INP expression increased the freezing point of the E. coli culture by a few degrees. Deletion of FabH, an important enzyme in fatty acid biosynthesis, significantly inhibited the ice-nucleation activity. Increased unsaturated fatty acids in the fabH mutant cells decreased the ice-nucleation activity. Adding exogenous saturated fatty acids increased both E. coli fatty acid saturation and the ice-nucleation activity. In contrast, adding unsaturated fatty acids exhibited the opposite effects. Furthermore, an E. coli MG1655-fadR strain with high saturated fatty acids content was constructed, in which the INP activity was enhanced by about 17% compared with its activity in the wild-type MG1655 strain.

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Purification and properties of an ice-nucleating protein from an ice-nucleating bacterium, Pantoea ananatis KUIN-3

Cited by 14 publications

References 27 publications

Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Functional display of ice nucleation protein InaZ on the surface of bacterial ghosts

Ice‐active characteristics of soil bacteria selected by ice‐affinity

High saturated fatty acids proportion inEscherichia colienhances the activity of ice-nucleation protein fromPantoea ananatis

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