Purification and properties of a glutathione peroxidase from Southern bluefin tuna (Thunnus maccoyii) liver

Thompson, Janene Lee; Thomas, P.; Schuller, Kathryn A.

doi:10.1016/j.cbpb.2006.01.011

Cited by 25 publications

(7 citation statements)

References 25 publications

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“…K m and V max values were 6.8 lM and 1.25 s -1 for hydrogen peroxide. The K m value for hydrogen peroxide was lower than that of other known GPx1s: 83.3 lM for human liver GPx1 [27], 12.4 lM for southern bluefin tuna liver GPx1 [16], and 10 lM for rainbow trout liver GPx1 [17]. We also found that K m values of Pacific bluefin tuna GPx1 for cumene hydroperoxide and t-butyl hydroperoxide were 16.0 and 7.0 lM, respectively.…”

Section: Discussionmentioning

confidence: 48%

“…We also found that K m values of Pacific bluefin tuna GPx1 for cumene hydroperoxide and t-butyl hydroperoxide were 16.0 and 7.0 lM, respectively. These values were lower than those for human GPx1 (27.8 lM for cumene hydroperoxide and 33.3 lM for t-butyl hydroperoxide) [27], southern bluefin tuna GPx1 (90 lM for cumene hydroperoxide and 90 lM for t-butyl hydroperoxide) [16], and rainbow trout GPx1 (48 lM for cumene hydroperoxide and 3000 lM for t-butyl hydroperoxide) [17]. These properties indicate that the purified enzyme is GPx1.…”

Section: Discussionmentioning

confidence: 73%

“…Food sources include fish and other seafood, red meat, eggs, chicken, and liver [3]. Selenium is incorporated in selenocysteine residues during protein synthesis to produce antioxidant enzymes such as GPx [4][5][6][7][8][9][10][11][12][13][14][15][16][17], thioredoxin reductase [18], and selenoprotein P [19].…”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

et al. 2012

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Glutathione peroxidase GPx1 was purified from the red muscle of Pacific bluefin tuna Thunnus orientalis and its enzymatic properties were characterized. The enzyme was optimally active at pH 7.4 with a specific activity for hydrogen peroxide and a K m of 6.7 lM. cDNA was also isolated and it contained a predicted open reading frame (ORF) encoding a 188 amino acid protein. The phylogenetic tree shows that fish including Pacific bluefin tuna, pufferfish, and zebrafish, but not mammals, possess two genetically distinct types of GPx1, i.e., GPx1a and GPx1b. The purified enzyme was classified as a fish GPx-1b enzyme on the basis of the phylogenetic tree of the GPx1 family.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 73%

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Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

et al. 2012

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“…SOD first catalyzes the dismutation of superoxide radicals to H 2 O 2 , which is further metabolized to H 2 O and O 2 by CAT and GPX [ 84 ]. GPX, a selenoprotein, plays an important role in detoxifying lipids and hydrogen peroxide, with the concomitant oxidation of glutathione [ 85 ]. In this study, compared with MNON, SOD and GPX gene expressions in MNOP were significantly down-regulated and up-regulated respectively, while CAT gene expression had no significant difference between these two groups.…”

Section: Discussionmentioning

confidence: 99%

Insights into Sexual Precocity of Female Oriental River Prawn Macrobrachium nipponense through Transcriptome Analysis

Jiang

Sun

et al. 2016

PLoS ONE

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BackgroundThe oriental river prawn (Macrobrachium nipponense) is the most prevalent aquaculture species in China. The sexual precocity in this species has received considerable attention in recent years because more and more individuals matured at a small size, which devalues the commercial production. In this study, we developed deep-coverage transcriptomic sequencing data for the ovaries of sexually precocious and normal sexually mature M. nipponense using next-generation RNA sequencing technology and attempted to provide the first insight into the molecular regulatory mechanism of sexual precocity in this species.ResultsA total of 63,336 unigenes were produced from the ovarian cDNA libraries of sexually precocious and normal sexually mature M. nipponense using Illumina HiSeq 2500 platform. Through BLASTX searches against the NR, STRING, Pfam, Swissprot and KEGG databases, 15,134 unigenes were annotated, accounting for 23.89% of the total unigenes. 5,195 and 3,227 matched unigenes were categorized by GO and COG analysis respectively. 15,908 unigenes were consequently mapped into 332 KEGG pathways, and many reproduction-related pathways and genes were identified. Moreover, 26,008 SSRs were identified from 18,133 unigenes. 80,529 and 80,516 SNPs were yielded from ovarian libraries of sexually precocious and normal sexually mature prawn, respectively, and 29,851 potential SNPs between these two groups were also predicted. After comparing the ovarian libraries of sexually precocious and normal sexually mature prawn, 549 differentially expressed genes (DEGs) and 9 key DEGs that may be related to sexual precocity of M. nipponense were identified. 20 DEGs were selected for validation by quantitative real-time PCR (QPCR) and 19 DEGs show consistent expression between QPCR and RNAseq-based differential expression analysis datasets.ConclusionThis is the first report on the large-scale RNA sequencing of ovaries of sexually precocious and normal sexually mature M. nipponense. The annotated transcriptome data will provide fundamental support for future research into the reproduction biology of M. nipponense. The large number of candidate SNPs and SSRs detected in this study could be used as genetic markers for population genetics and functional genomics in this species. More importantly, many DEGs, especially nine key DEGs between sexually precocious and normal sexually mature prawns were identified, which will dramatically improve understanding of molecular regulatory mechanism of sexual precocity of this species.

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“…Fish GPx is purified in the liver of the Southern bluefin tuna (Thunnus maccoyii), which is present in a homotetramer with a native molecular mass of 85 kDa and a subunit molecular mass of approximately 24 kDa. 23) The Southern bluefin tuna GPx is structurally similar to the classical GPx-1 found in the liver and red blood cells, the gastrointestinal GPx-2 and the plasma GPx-3 of mammals.…”

Section: Selenoproteins In Fish and Shellfishmentioning

confidence: 95%

Selenium in Seafood Materials

Yoshida

Haratake

Fuchigami

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Research interests in studying the biochemical nature of selenium have increased and the importance of this element as an essential micronutrient in many organisms has been well recognized. Selenium occurs in proteins in the form of the 21st amino acid, selenocysteine (SeCys or Sec). In this review, we describe the speciation analysis of the fish-specific selenoproteins and non-proteinous selenium compounds, and the nutritional bioavailability of selenium from seafood materials. Selenium is essential to fish and shellfish. The selenoproteomes (sets of SeCys-containing proteins) of fish are greater in number than those of mammals (25 selenoproteins in humans); at 30-37 selenoproteins, the selenoproteomes of fish are among the largest known. The same core selenoprotein families are found in mammals and fish. In addition, fish have several species-specific selenoproteins [fish 15 kDa selenoprotein-like protein (Fep15), selenoprotein J and selenoprotein L] that are missing in mammals. Actually, not only proteinous selenium species like selenomethionine (SeMet) and SeCys derivatives, but also many non-proteinous organic ones were detected in fish and shellfish samples. Although the selenium contents in seafood are higher than in terrestrial foodstuffs, little is known about the chemical forms of organoselenium species in seafood. The nutritional bioavailability of selenium from seafood appears to be dependent on the fish and shellfish species and/or place where they are produced; some seafood gives rise to a high bioavailability of selenium, which is comparable to that of wheat and beef. Fish and shellfish materials are major dietary sources of selenium for the Japanese population (∼ 60% of daily intake). Seafood materials appear to contain nutritionally effective organoselenium compounds that have not yet been chemically identified.

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Purification and properties of a glutathione peroxidase from Southern bluefin tuna (Thunnus maccoyii) liver

Cited by 25 publications

References 25 publications

Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis

Insights into Sexual Precocity of Female Oriental River Prawn Macrobrachium nipponense through Transcriptome Analysis

Selenium in Seafood Materials

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