Purification and Properties of a Pectintrans-Eliminase inErwin aroideaeFormed in the Presence of Nalidixic Acid

Purification and some properties of the pectin lyase from Penicillium italicum

“…Finally, pectin lyase from P. #alicum showed higher thermostability than some pectin lyases described not only in fungi [10,12,23] but also in bacteria [16,29], ex. cept two isoenzymes partially purified from Au.…”

Purification, Characterization, and Production of Two Pectic Transeliminases with Protopectinase Activity fromBacillus subtilis

“…The production of bacterial pectin lyases is closely related to the culture conditions. Pectin lyases from E. carotovora,18) E. chrysanthemi, 19) E. aroideae, 20) and P. marginalis 21 ) are produced using DNA-damaging agents, and that of P. jiuorescens 22 ) is induced by the addition of glycerol to the culture medium. However, PPase-R was not produced in B. subtilis IFO 3134 using mitomycin C or glycerol in the same culture conditions reported for the above mentioned bacteria (data not shown).…”

Enzymological and Immunological Properties of Pectin Lyases from Bacteriocinogenic Strains ofErwinia carotovora

“…Strains of E. carotovora sensitive to carotovoricins from the four bacteriocinogenic strains were described previously. 8 5 l with a slight modification. Unless otherwise noted, the organisms were grown in M9-glucose-casein acid hydrolysate medium 7 l at 30°C with shaking.…”

“…4 l reported that pectolytic enzyme formation in Erwinia carotovora Er (formerly termed E. aroideae Er) was markedly stimulated when the cells were treated with nalidixic acid, mitomycin C, bleomycin or UV light irradiation. The pectolytic enzyme formed by E. carotovora Er treated with nalidixic acid has been purified and characterized as a pectin lyase, 5 l while this strain produces peetate lyase when polygalacturonic acid or pectin was added to the cultural medium as an inducer.6l Tomizawa et af. 4 l also reported that E. carotovora Er formed a phage tail-like substance as well as pectin lyase accompanied by cell lysis in the presence of the drugs or after exposure to UV light.…”