Purification and properties of a manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis

The spoIVC locus of Bacillus subtilis was analysed. Fourteen spoIVC mutants isolated following nitrosoguanidine mutagenesis were used along with two previously characterized spoIVC mutants to construct a fine structure genetic map of the locus. The recombination index (RI) measured between extreme mutations was 0.26; no recombination could be detected between four of the mutations. Complementation analysis showed that all the mutations fall into two cistrons. The RI between extreme mutations in cistron A was about 0.17 and that between extreme mutations in cistron B was about 0.05. In respect of biochemical markers, the spoIVC mutations all produced similar phenotypes, irrespective of their location. However, in both cistrons oligosporogenous and asporogenous mutations mapped close together.

“…(iii) Manganese-dependent DNAase was assayed ( I s ) as described by Akrigg (1978). One unit of DNAase produces 1 nmol of acid-soluble nucleotides in 20 min at 37 "C.…”

Section: Organisms Bacillus Subtilismentioning

confidence: 99%

Phenotypic and Genetic Characterization of Mutations in the spoIVC Locus of Bacillus subtilis

Farquhar

Yudkin

1988

“…E R R I N G T O N AND J. MANDELSTAM phosphate in the supernatant was determined by measuring the A,,o. One unit of enzyme is defined as the amount that hydrolyses one nmolp-nitrophenol phosphate in 1 min at 30 "C. Manganese-dependent DNAase was assayed as described by Akrigg (1978). One unit of DNAase is defined as the amount that produces 1 nmol of acid-soluble nucleotides in 20 min at 37 "C. Glucose dehydrogenase was assayed as described by Sadoff (1966).…”

Section: Frequency Of Spo+ Among Lys+ Transformants (Spa Dna)mentioning

confidence: 99%

Variety of Sporulation Phenotypes Resulting from Mutations in a Single Regulatory Locus, spoIIA, in Bacillus subtilis

Errington

Mandelstam

1983

Closely linked mutations in either of the two putative genes of the sporulation locus spoIIA can affect, in quite diverse ways, spore incidence, the production of alkaline phosphatase and DNAase, and the stability of the cells in sporulation medium. It is concluded that the locus has a regulatory function affecting the activation or induction of at least two, and possibly more, sporulation-associated operons.

“…The spoIIA locus is particularly interesting as mutations in it lead to various phenotypes, including asporogeny or oligosporogeny, and a lack of production of several enzymes (Ionesco & Schaeffer, 1968;Coote, 1972a,b;Piggot, 1973;Akrigg & Mandelstam 1978;Yudkin & Turley, 1980;Errington & Mandelstam, 1983). A part of the locus was cloned in a plasmid and was shown to complement mutations at one end of the locus .…”

Section: Introductionmentioning

confidence: 99%

Nucleotide Sequence of Sporulation Locus spoIIA in Bacillus subtilis

Fort¹,

Piggot²

1984

102

We have determined a sequence of 2073 bp from two recombinant plasmids carrying the whole spoIIA locus from Bacillus subtilis, the expression of which is required for spore formation. The sequence contains three long open reading frames (ORFs), each of them being preceded by a ribosome binding site. These three putative proteins (mol. wts 13 100, 16300 and 22200) are likely to be expressed and are probably encoded on the same mRNA. The stop d o n of ORFl overlaps with the start d o n of ORF2 suggesting that there might be translational coupling between the two ORFs. Although some known promoter sequences were found, the only one upstream from the first open reading frame is about 260 bp from it.