Purification and Properties of a Carbonyl Reductase Involved in Stereoselective Reduction of Ethyl 4-Chloro-3-oxobutanoate fromCylindrocarpon sclerotigenumIFO 31855

Abstract

A NADPH-dependent carbonyl reductase (CSCR1) was purified to homogeneity from Cylindrocarpon sclerotigenum IFO 31855. The enzyme catalyzed the stereoselective reduction of ethyl 4-chloro-3-oxobutanoate to the corresponding (S)-alcohol with a >99% enantiomer excess. The relative molecular mass of the enzyme was estimated to be 68,000 by gel filtration chromatography and 24,800 on SDS polyacrylamide gel electrophoresis. The enzyme had an extremely narrow substrate specificity and it highly reduced conjugated diketone, 2,3-butanedion, in addition to ethyl 4-chloro-3-oxobutanoate. The enzyme activity was inhibited by HgCl(2) (100%), 5,5'-dithiobis(2-nitrobenzoic acid) (56%), dicoumarol (42%), and CuSO(4) (46%). The N-terminal amino acid sequence of the enzyme (P-Q-G-I-P-T-A-S-R-L) showed no apparent similarity with those of other oxidoreductases.

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“…Cylindrocarpon sclerotigenum IFO 31855 SDR low promiscuity Incomplete [116] ALR Sporobolomyces salmonicolor ZJUB 105 DUF4126 low promiscuity SSU26463 [117] Hketo Hansenula polymorpha SDR promiscuous XP_013937475 [118,119] LsADH Leifsonia sp. strain S749 SDR multiple substrates AB213459 [120] SmCR…”