Purification and Physicochemical Characteristics of African Swine Fever Virus

Black, D. N.; Brown, F.

doi:10.1099/0022-1317-32-3-509

Cited by 30 publications

(14 citation statements)

References 14 publications

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“…The number of polypeptides does not agree with that given by others (3,22), probably because they worked with extracellular virus, which cannot contain some proteins such as VP 15, a major component of intracellular virus. The low concentration of most of the minor polypeptides might be hidden in the high background of densitometry (3).…”

Section: I S E U S S I O Nmentioning

confidence: 54%

Proteins specified by African swine fever virus

Tabarés

Marcotegui

Fernández

et al. 1980

Archives of Virology

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At least 28 polypeptides have been identified in intracellular virus, with molecular weights ranging from 11,500 to 243,000 daltons. By treatment with Nonidet P-40 and 2-mercaptoethanol it is possible to obtain subviral particles that have lost some proteins and have a density in CsCl of 1.31 g/cm3 which is higher than that of the complete virus (1.23 g/cm3). After addition of NaCL the virus loses its major protein VP73 which indicates that it is localized in the viral envelope. Cores obtained after this treatment are made up of at least 14 proteins. Incorporation of 3H-fucose and 3H-glucosamine in intracellular virus occurs in three minor components. The protein VP42 is possibly the cell actin and appears to be strongly associated with the virus. It is not possible to eliminate it under conditions where the viral envelopes disappear morphologically. At least the proteins VP172, VP162, VP146 and VP73 act as antigens in the natural infection.

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Section: I S E U S S I O Nmentioning

confidence: 54%

Proteins specified by African swine fever virus

Tabarés

Marcotegui

Fernández

et al. 1980

Archives of Virology

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“…Previous studies of ASFV proteins and antigen-antibody reactions have used virus or viral proteins produced in non-porcine cells (19) or porcine cells not usually considered to be the host of viral replication in vivo (2). Recent studies of ASFV DNA revealed extensive changes in Sma I and Eeo 1%I restriction patterns associated with adaptation of the virus to growth in Veto cells (20).…”

Section: Discussionmentioning

confidence: 99%

Characterization of African swine fever virus antigenic proteins by immunoprecipitation

Letchworth

Whyard

1984

Archives of Virology

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African swine fever virus is a large, complex virion in which numerous proteins have been identified by biochemical techniques. Few of these proteins have been shown to react with antibodies from recovered swine, leading to speculation that the immunological unreactivity of some viral proteins might explain the inability of immune sera from surviving animals to neutralize the virus. We used immunoprecipitation of radiolabeled viral proteins to examine these sera in more detail. Gradient sodium dodecyl sulphate polyacrylamide gel electrophoretic analysis of these immunoprecipitates revealed that at least 37 viral proteins participated in antigen-antibody reactions in this system. Differences in the molecular weights of some immunoprecipitable proteins were noted between different isolates of virus, between the same isolate grown in different cells, and between an isolate adapted to Vero cells and one not adapted to these cells.

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“…Available information from polyacrylamide gel electrophoresis suggests that the number of polypeptides present in virus particles of ICDVs is quite different and not correspondingly related to virus size. African swine fever virus apparently contains only five structural proteins (Black and Brown, 1976). Frog virus 3 contains about 20 structural polypeptides Goorha and Granoff, 1974a;Willis et al, 1977), the most prevalent having a molecular weight of 55,000 and accounting for nearly 26% of the total proteins.…”

Section: Proteinsmentioning

confidence: 97%