Purification and Partial Characterization of a Virus from Pineapple

Gunasinghe, U. B.; German, Thomas L.

doi:10.1094/phyto-79-1337

Cited by 34 publications

(18 citation statements)

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“…In 1989, filamentous virions typical of a member of the family Closteroviridae (subsequently referred to as closterovirids), and dsRNA, a replicative intermediate of many genera of plant viruses, were detected in pineapple (Gunasinghe and German 1989). Using monoclonal antibodies, two serotypes of the virus were detected by immunosorbent electron microscopy (ISEM) (Hu et al 1996).…”

Section: Introductionmentioning

confidence: 99%

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Gambley

Steele²,

Geering³

et al. 2008

Austral. Plant Pathol.

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Abstract. Pineapple mealybug wilt-associated virus 1 (PMWaV-1), 2 (PMWaV-2) and 3 (PMWaV-3) have been detected in Australian commercial pineapple crops, along with a previously undescribed ampelovirus, for which the name Pineapple mealybug wilt-associated virus 5 (PMWaV-5) is proposed. Partial sequences extending from open reading frame 1b through to the heat shock protein homologue were obtained for PMWaV-1, -3 and -5. Phylogenetic analyses of selected regions of these sequences indicated that PMWaV-5 is a distinct species and most closely related to PMWaV-1. The amino acid sequence variation observed in the RNA-dependent RNA polymerase region of PMWaV-1 isolates was 95.8-98.4% and of PMWaV-3 isolates was 92.2-99.5%.In surveys of mealybug wilt disease (MWD) affected crops, none of the four viruses was clearly associated with the disease at all survey sites. A statistically significant association (P < 0.001) between the presence of PMWaV-2 and symptoms was observed at one survey site (site 3), but the virus was at a low incidence at the remaining three survey sites. By contrast, although PMWaV-1 and -3 were equally distributed between symptomless and MWD-affected plants at site 3, there was a statistically significant (P < 0.001) association between each of these two viruses and MWD at sites 1 and 4. At site 2, there was a statistically significant (P < 0.001) association only between PMWaV-3 and MWD. PMWaV-1 was the most commonly found of the four viruses and conversely PMWaV-5 was only occasionally found. Australian isolates of PMWaV-1, -2 and -3 were transmitted by the mealybug species Dysmicoccus brevipes.

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Section: Introductionmentioning

confidence: 99%

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Gambley

Steele²,

Geering³

et al. 2008

Austral. Plant Pathol.

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“…DRAFT OF CLOSTEROVIRUS TAXONOMY Until recently, the taxonomic status of closteroviruses has been illdefined, and the group was a quite heterogeneous collection, with the main distinction being between the so-called typical and atypical closteroviruses (Bar-Joseph et al, 1979;Bar-Joseph and Murant, 1982;Francki et al, 1991). Former closteroviruses such as apple chlorotic leafspot virus, apple stem grooving virus and citrus tatter leaf virus have already been reclassified as trichoviruses and capilloviruses, respectively (Francki et al, 1991; Gunasinghe and German (1989) Yes Lockhart et al (1992) Yes Inouye (1976) Yes Yes Duffus et al (1986); Klaasen et al Atypical (or "clostero-like") viruses have shorter particles of about 750 nm, do not induce BYV-type vesicles, and have no known vector.…”

Section: Introductionmentioning

confidence: 99%

Principles of Molecular Organization, Expression, and Evolution of Closteroviruses: Over The Barriers

1996

Advances in Virus Research

105

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“…Contudo, quando ocorre um grande aumento de sua população, podem ser também observados nas inflorescências, nos pedúnculos, nas infrutescências do abacaxi e nas mudas que crescem ao redor da infrutescência (SANTA-CECÍLIA e CHALFOUN, 1998). A cochonilha ocasiona o enfraquecimento das plantas devido à sucção da seiva, além de estar associada a uma doença de origem virótica conhecida como murcha-doabacaxizeiro (GUNASHINGHE e GERMAN, 1987;1989), a qual impede a frutificação normal, podendo ocasionar também a morte das plantas antes do período reprodutivo; os prejuízos podem ultrapassar os 50% (SANCHES, 1997). Assim, esse complexo, c ochonilha e murcha-do-abacaxizeiro, tem-se constituído em um dos maiores entraves para o aumento da produtividade da cultura no Brasil.…”

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Desenvolvimento de Dysmicoccus brevipes (Cockerell) (Hemiptera:Pseudococcidae) em duas cultivares de abacaxi

Santa-Cecília

Bueno²,

Prado

2004

Ciênc. agrotec.

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RESUMOA cochonilha Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae) é uma das principais pragas em cultivos de abacaxi e os estudos biológicos desse inseto em diferentes cultivares são uma necessidade. Objetivou-se com este trabalho avaliar o desenvolvimento dessa cochonilha em duas cultivares de abacaxi, Pérola e Cayenne. O experimento foi conduzido em câmara climatizada a 25 ± 1ºC, 70 ± 10% UR e fotofase de 12 horas. As plantas de abacaxi foram reproduzidas in vitro, transplantadas para vasos (250 mL) e aclimatadas em casa-de-vegetação. Ninfas com até um dia de vida foram confinadas em gaiolas de PVC (1 cm de diâmetro), e fixadas em folhas de ambas as cultivares. Não foram detectadas diferenças no desenvolvimento de D. brevipes nos dois substratos alimentares utilizados. O desenvolvimento ninfal de fêmeas e machos de D. brevipes foi 39,9 e 32,0 dias na cv. Pérola e 38,5 e 32,4 dias na cv. Cayenne, respectivamente. A sobrevivência da fase ninfal foi 32,3 e 40,5%; a razão sexual, 0,39 e 0,33; e a longevidade de fêmeas, 20,3 e 26,1 dias nas cvs. Pérola e Cayenne, respectivamente. Termos para indexação:Insecta, cochonilha-do-abacaxi, Ananas comosus (L.) Merril, biologia. ABSTRACTThe pineapple mealybug Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae) is the main pest on pineapple crops and biological studies of this insect are necessary on different cultivars. The objective of this work was to evaluate the development time of this mealybug on the pineapple cultivars Pérola and Cayenne. The experiment was performed in climated chambers at 25 ± 1ºC, 70 ± 10% RH and 12 h photophase. Pinneapple plants were reproduced in vitro, transplanted to pots (250 mL) and kept in greenhouse. One-day-old nymphs were kept inside a clipcage (1 cm diameter) fixed on the plants. No differences in development of D. brevipes among the cultivars were found. Development times of females and males, respectively, were 39.9 and 32 days on cv. Pérola and 38.5 and 32.4 days on cv. Cayenne. Nymphs survivals were 32.3 and 40.5%, sexual rates were 0.39 and 0.33, and females longevities were 20.3 and 26.1 days on cv. Pérola and Cayenne, respectively. Index terms: INTRODUÇÃOA cochonilha Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae) vem ocorrendo nos abacaxizais do Brasil desde 1931, sendo seus prejuízos a causa do declínio da cultura em muitas áreas do Estado de São Paulo e, posteriormente, de Minas Gerais (HAMBLETON, 1935; SANTA-CECÍLIA e CHALFOUN, 1998).Adultos e ninfas vivem em colônias e localizam-se nas raízes e axilas das folhas. Contudo, quando ocorre um grande aumento de sua população, podem ser também observados nas inflorescências, nos pedúnculos, nas infrutescências do abacaxi e nas mudas que crescem ao redor da infrutescência (SANTA-CECÍLIA e CHALFOUN, 1998). A cochonilha ocasiona o enfraquecimento das plantas devido à sucção da seiva, além de estar associada a uma doença de origem virótica conhecida como murcha-doabacaxizeiro (GUNASHINGHE e GERMAN, 1987;1989), a qual impede a frutificação normal, ...

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Purification and Partial Characterization of a Virus from Pineapple

Cited by 34 publications

References 0 publications

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Principles of Molecular Organization, Expression, and Evolution of Closteroviruses: Over The Barriers

Desenvolvimento de Dysmicoccus brevipes (Cockerell) (Hemiptera:Pseudococcidae) em duas cultivares de abacaxi

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