Purification and Partial Characterization of α‐Factor, a Mating‐Type Specific Inhibitor of Cell Reproduction from <i>Saccharomyces cerevisiae</i>

Duntze, W.; Stötzler, Dieter; Bücking-Throm, Elizabeth; Kalbitzer, Sigrid

doi:10.1111/j.1432-1033.1973.tb02847.x

Cited by 126 publications

(85 citation statements)

References 8 publications

(10 reference statements)

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“…a-Factor: From a culture of X2180-1B, a-factor was partially purified according to the procedure described by Duntze et al . (6). The biological activity of a-factor was determined by the agar diffusion assay method of Duntze et al (6).…”

Section: Methodsmentioning

confidence: 99%

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Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.

Iida¹,

Yahara²

1984

The Journal of Cell Biology

102

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Growth arrests of Saccharomyces cerevisiae cells in early G, phase brought by various means were classified into two types according to the mode of growth recovery after release of the restraints against growth . The first type, including arrests caused by cdc25, cdc33, cdc35, and ilsl mutations at the nonpermissive temperature and also by sulfur starvation, showed a subsequent delay in the onset of budding when shifted back to permissive conditions . The length of the delay was positively correlated with the time that cells had been arrested . The second type, including those caused by cdc28 and cdc24 mutations and by a factor, did not affect the mode of growth recovery after the shift to permissive conditions irrespective of the time that cell proliferation had been restricted .

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Section: Methodsmentioning

confidence: 99%

“…(6). The biological activity of a-factor was determined by the agar diffusion assay method of Duntze et al (6).…”

Section: Methodsmentioning

confidence: 99%

Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.

Iida¹,

Yahara²

1984

The Journal of Cell Biology

102

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“…It has been observed earlier that the culture filtrates of a cells contain at least two separable compounds exhibiting a factor activity [3]. Of these the major compound has been isolated and characterized as a low molecular weight peptide [3].…”

Section: Enzymesmentioning

confidence: 98%

“…The cells were grown aerobically at 30 "C in the liquid minimal salt medium described previously [3]. The isogenic strain X2180-1A (mating type a ) was used as an indicator strain for the determination of a factor activity.…”

Section: Yeast Strains and Culture Conditionsmentioning

confidence: 99%

Isolation and Characterization of Four Related Peptides Exhibiting alpha Factor Activity from Saccharomyces cerevisiae

Stötzler

Duntze

1976

Eur J Biochem

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The molecular structure of a factor, a mating hormone produced by a mating type cells of Saccharomyces cerevisiae has been investigated. From culture filtrates of a cells four oligopeptides exhibiting a factor activity have been isolated. These peptides, designated as a 1, a 2 , a3, and a 4 are structurally closely related, being composed of thirteen (a1 and a3) and twelve ( a 2 and a4) amino acids, respectively. The peptides were found to be composed of the following amino acids: 2 glutamic acid or glutamine, 2 proline, 1 glycine, 1 methionine or methionine sulfoxide, 2 leucine, 1 tyrosine, 1 lysine, 1 histidine, 1 or 2 tryptophan. The tridekapeptides differ from the dodekapeptides by an additional NH2-terminal tryptophan residue. Tyrosine was identified as the C-terminal amino acid in all four peptides. a3 and a4 are oxidation products containing an internal methionine sulfoxide instead of methionine. The mechanisms which could introduce the observed heterogeneity of the peptides are discussed.In the life cycle of the yeast Saccharomyces cerevisiae the transition from the haploid to the diploid growth phase is achieved by the conjugation of two haploid cells of different mating type. This process appears to be controlled by specific regulatory factors which are now generally referred to as mating hormones or sexual hormones (for review see [l]). Haploid yeast cells belong to either one of two alternative mating types designated as a and a, each of which produces one or, probably, several hormones which are excreted into the culture medium and act specifically on cells of the opposite mating type [2-51. Thus, culture filtrates of a cells contain an activity designated as a factor which specifically inhibits cell division in cells of mating type a [2]. A corresponding a factor which is produced by a cells and acts on a cells has been recently described by several groups [4,5]. It has been shown that a factor interferes with an essential cell cycle function of the a cells arresting them at an early stage of the cycle during the G1 period [6]. As a consequence initiation of DNA synthesis and bud formation are inhibited [6,7]. Since the mating ability of haploid yeast cells is restricted to the early part of the cell cycle it has been proposed that under physiological conditions the hormones could serve to increase the fraction of competent cells in a mating mixture, thus enhancing the efficiency of conjugation [5,8].

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“…In one such experiment the culture was divided into two samples when the percentage of budded cells had reached 39%. a-Factor was added to one sample to a final concentration of 10 units ml-l (for a definition of units see, Duntze et al, 1973); the other portion was left undisturbed and used as a control. The percentage of budded cells in the a-factor treated culture reached a plateau value of 58% after 50 min (Fig.…”

Section: R E S U L T Smentioning

confidence: 99%

Cell Cycle Initiation and Bud Emergence in Saccharomyces cerevisiae

Lörincz¹,

Carter

1983

Microbiology

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Purification and Partial Characterization of α‐Factor, a Mating‐Type Specific Inhibitor of Cell Reproduction from Saccharomyces cerevisiae

Cited by 126 publications

References 8 publications

Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.

Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.

Isolation and Characterization of Four Related Peptides Exhibiting alpha Factor Activity from Saccharomyces cerevisiae

Cell Cycle Initiation and Bud Emergence in Saccharomyces cerevisiae

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