Purification and Kinetic Characterization of Hepatic Catalase from Carnivore Fish, Channa striata Exposed to Agrochemicals (endosulfan+deltamethrin)

Tufail, Fatima; Abdullah, Sajid; Naz, Huma; Abbas, Khalid; Shafique, Laiba

doi:10.17582/journal.pujz/2019.34.1.79.84

Cited by 2 publications

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“…Mohanty et al (2013) reported the 32.17% reduction in total protein contents of L. rohita under chronic metal's stress. Significantly lower protein contents in liver of fish under toxicants exposure was also noted by Tufail et al (2019).…”

Section: Figure 4 Impact Of Nbt Concentration On Liver Sod Activity Of Control (A) and Pb+cr (B) Exposed L Rohitamentioning

confidence: 70%

Thermal stability of purified superoxide dismutase from liver of commercially valuable fish, Labeo rohita Exposed to Pb+Cr mixture

Naz¹

2021

PAKJAS

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In this experiment, effect of lead (Pb) + chromium (Cr) mixture on superoxide dismutase (SOD) in the liver of Labeo rohita at a concentration of 11.1 mgL-1 was observed. The ammonium sulphate precipitation and ion exchange chromatography techniques were successfully used to purify SOD. After purification, SOD activity of control and Pb+Cr treated fish was noted as 581.00 and 645.45 UmL-1, respectively while the specific activity was 1383.33 and 1613.62 Umg-1, respectively. The fold purification value of SOD was 2.75 and 2.45 for control and stressed fish, respectively. The recovery was calculated as 77.06 and 57.43% for control and stressed fish, respectively. The results of kinetic characterization showed that SOD form control and exposed fish had maximum activity at pH 6.5 and 7.0. Temperature also had a significant effect on activity of SOD. The SOD activity was measured maximum at 30°C for both control and Pb+Cr exposed fish. The Km value of liver SOD for control and Pb+Cr treated L. rohita was calculated as 1.48 and 0.62 mM, respectively. The value of Vmax for SOD from liver of control and Pb+Cr exposed fish was 1000 and 570 U mL-1, respectively. The enthalpy of denaturation (∆H*) for liver SOD from control and Pb+Cr exposed L. rohita was computed as 3.492 and 2.802 KJ mol-1 at 40°C, respectively and these values were dropped off with increasing the temperature until it remains 3.251 and 2.561 KJ mol-1 at 70°C, respectively. The free energy of thermal denaturation (ΔGº) of liver SOD was slightly increased with increasing temperature until 75°C which shows its resistance against heat. The values of ΔGº was observed as 58.03 and 57.95 KJ mol-1 for control and exposed fish at 40°C, respectively while the same was increased upto 62.37 and 62.00 KJ mol-1 at 70°C, respectively. It was concluded from negative value of ΔS* (entropy of inactivation) that the SOD is stable thermodynamically.

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Section: Figure 4 Impact Of Nbt Concentration On Liver Sod Activity Of Control (A) and Pb+cr (B) Exposed L Rohitamentioning

confidence: 70%

Thermal stability of purified superoxide dismutase from liver of commercially valuable fish, Labeo rohita Exposed to Pb+Cr mixture

Naz¹

2021

PAKJAS

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Catalase Activity in Response to Metals in Freshwater Fish Catla catla

2020

AJLS

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Catalase activity in response to binary metal mixture of Zn + Ni was studied in gills, hepatic, renal and cardiac tissues of Catla catla. A total of 30 fish fingerlings were kept in two glass aquaria (15 in controlled condition and 15 in metal mixture containing aquarium) for the period of two weeks. After the completion of the trial period, fish were dissected for different organ collection. The extracted organs were homogenized in phosphate buffer (50 mM; pH 7.0). The activity of catalase was determined by absorption at 240 nm by using the standard methods. The inferences showed higher catalase activity in liver (223.33 ±1 UmL-1), kidney (163.33 ± 0.7 UmL-1), gills (123.33 ±0.9 UmL-1) and cardiac (120 ±3 UmL-1) tissues of Zn + Ni treated fish in comparison to controlled fish liver (116.66 ±2 UmL-1), kidney (101.66 ±1 UmL-1), gills (96.66 ±0.66 UmL-1) and cardiac tissues (70 ±0.33 UmL-1) in this study. Statistically, significant differences at p ≤ 0.05 was observed for catalase activity between Zn + Ni stressed and control fish groups. While, in different organs of both group of fishes the catalase activity order was observed as hepatic > renal > gills > heart. Findings of this study would be helpful in monitoring aquatic ecosystems using fish antioxidant system which acts as a bio-indicator of metal contamination.

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Purification and Kinetic Characterization of Hepatic Catalase from Carnivore Fish, Channa striata Exposed to Agrochemicals (endosulfan+deltamethrin)

Cited by 2 publications

References 0 publications

Thermal stability of purified superoxide dismutase from liver of commercially valuable fish, Labeo rohita Exposed to Pb+Cr mixture

Thermal stability of purified superoxide dismutase from liver of commercially valuable fish, Labeo rohita Exposed to Pb+Cr mixture

Catalase Activity in Response to Metals in Freshwater Fish Catla catla

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